Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01190 and RR42_RS01195 are separated by 75 nucleotides RR42_RS01195 and RR42_RS01200 are separated by 64 nucleotides RR42_RS01200 and RR42_RS01205 are separated by 29 nucleotides RR42_RS01205 and RR42_RS01210 are separated by 145 nucleotides
RR42_RS01190: RR42_RS01190 - peptidase M48, at 272,775 to 273,722
_RS01190
RR42_RS01195: RR42_RS01195 - excinuclease ATPase, at 273,798 to 274,271
_RS01195
RR42_RS01200: RR42_RS01200 - hypothetical protein, at 274,336 to 275,145
_RS01200
RR42_RS01205: RR42_RS01205 - transposase, at 275,175 to 275,579
_RS01205
RR42_RS01210: RR42_RS01210 - n-acetyl-gamma-glutamyl-phosphate reductase, at 275,725 to 276,672
_RS01210
Position (kb) 274
275
276 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 273.344 kb on + strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand at 273.345 kb on - strand, within RR42_RS01190 at 273.447 kb on + strand, within RR42_RS01190 at 273.653 kb on + strand at 273.713 kb on - strand at 273.720 kb on + strand at 273.759 kb on - strand at 273.759 kb on - strand at 273.763 kb on + strand at 273.763 kb on + strand at 273.764 kb on - strand at 273.764 kb on - strand at 273.767 kb on + strand at 273.767 kb on + strand at 274.200 kb on + strand, within RR42_RS01195 at 274.200 kb on + strand, within RR42_RS01195 at 274.201 kb on - strand, within RR42_RS01195 at 274.224 kb on + strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 275.617 kb on + strand at 275.617 kb on + strand at 275.703 kb on - strand at 275.703 kb on - strand at 275.710 kb on - strand at 275.718 kb on - strand at 275.966 kb on + strand, within RR42_RS01210 at 275.966 kb on + strand, within RR42_RS01210 at 275.967 kb on - strand, within RR42_RS01210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 273,344 + RR42_RS01190 0.60 +0.5 273,345 - RR42_RS01190 0.60 +0.2 273,345 - RR42_RS01190 0.60 +0.4 273,345 - +0.3 273,345 - RR42_RS01190 0.60 +1.0 273,447 + RR42_RS01190 0.71 +3.5 273,653 + +1.3 273,713 - +0.5 273,720 + +0.0 273,759 - +0.9 273,759 - -0.7 273,763 + +1.1 273,763 + +0.3 273,764 - -0.8 273,764 - +0.4 273,767 + -0.0 273,767 + +1.3 274,200 + RR42_RS01195 0.85 +1.8 274,200 + RR42_RS01195 0.85 +0.2 274,201 - RR42_RS01195 0.85 +0.3 274,224 + +0.9 274,305 - +1.1 274,305 - +0.8 274,305 - +0.5 274,305 - -0.0 274,305 - +0.7 275,617 + -1.0 275,617 + -0.7 275,703 - +0.1 275,703 - +0.9 275,710 - +0.9 275,718 - -0.3 275,966 + RR42_RS01210 0.25 -1.1 275,966 + RR42_RS01210 0.25 -2.0 275,967 - RR42_RS01210 0.26 -0.5
Or see this region's nucleotide sequence