Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01080 and RR42_RS01085 are separated by 23 nucleotides RR42_RS01085 and RR42_RS01090 overlap by 4 nucleotides RR42_RS01090 and RR42_RS01095 are separated by 214 nucleotides RR42_RS01095 and RR42_RS01100 are separated by 35 nucleotides
RR42_RS01080: RR42_RS01080 - hypothetical protein, at 253,333 to 253,629
_RS01080
RR42_RS01085: RR42_RS01085 - chemotaxis protein CheY, at 253,653 to 254,276
_RS01085
RR42_RS01090: RR42_RS01090 - histidine kinase, at 254,273 to 255,676
_RS01090
RR42_RS01095: RR42_RS01095 - membrane protein, at 255,891 to 256,370
_RS01095
RR42_RS01100: RR42_RS01100 - LysR family transcriptional regulator, at 256,406 to 257,341
_RS01100
Position (kb)
254
255
256 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 253.346 kb on + strand at 253.346 kb on + strand at 253.346 kb on + strand at 253.346 kb on + strand at 253.347 kb on - strand at 253.347 kb on - strand at 253.347 kb on - strand at 253.425 kb on - strand, within RR42_RS01080 at 253.609 kb on + strand at 253.609 kb on + strand at 253.610 kb on - strand at 253.610 kb on - strand at 253.610 kb on - strand at 253.610 kb on - strand at 253.610 kb on - strand at 253.637 kb on + strand at 253.638 kb on - strand at 253.654 kb on + strand at 253.655 kb on - strand at 253.706 kb on + strand at 253.706 kb on + strand at 253.706 kb on + strand at 253.706 kb on + strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.707 kb on - strand at 253.733 kb on + strand, within RR42_RS01085 at 253.733 kb on + strand, within RR42_RS01085 at 253.733 kb on + strand, within RR42_RS01085 at 253.733 kb on + strand, within RR42_RS01085 at 253.734 kb on - strand, within RR42_RS01085 at 253.894 kb on + strand, within RR42_RS01085 at 253.895 kb on - strand, within RR42_RS01085 at 253.895 kb on - strand, within RR42_RS01085 at 253.945 kb on + strand, within RR42_RS01085 at 253.945 kb on + strand, within RR42_RS01085 at 254.077 kb on + strand, within RR42_RS01085 at 254.077 kb on + strand, within RR42_RS01085 at 254.078 kb on - strand, within RR42_RS01085 at 254.433 kb on + strand, within RR42_RS01090 at 254.434 kb on - strand, within RR42_RS01090 at 254.754 kb on + strand, within RR42_RS01090 at 254.754 kb on + strand, within RR42_RS01090 at 254.754 kb on + strand, within RR42_RS01090 at 254.755 kb on - strand, within RR42_RS01090 at 254.755 kb on - strand, within RR42_RS01090 at 254.827 kb on - strand, within RR42_RS01090 at 254.874 kb on + strand, within RR42_RS01090 at 254.875 kb on - strand, within RR42_RS01090 at 254.875 kb on - strand, within RR42_RS01090 at 255.150 kb on + strand, within RR42_RS01090 at 255.150 kb on + strand, within RR42_RS01090 at 255.151 kb on - strand, within RR42_RS01090 at 255.151 kb on - strand, within RR42_RS01090 at 255.192 kb on + strand, within RR42_RS01090 at 255.192 kb on + strand, within RR42_RS01090 at 255.193 kb on - strand, within RR42_RS01090 at 255.193 kb on - strand, within RR42_RS01090 at 255.213 kb on + strand, within RR42_RS01090 at 255.213 kb on + strand, within RR42_RS01090 at 255.213 kb on + strand, within RR42_RS01090 at 255.213 kb on + strand, within RR42_RS01090 at 255.214 kb on - strand, within RR42_RS01090 at 255.229 kb on - strand, within RR42_RS01090 at 255.294 kb on + strand, within RR42_RS01090 at 255.295 kb on - strand, within RR42_RS01090 at 255.670 kb on + strand at 255.671 kb on - strand at 255.688 kb on - strand at 255.688 kb on - strand at 255.844 kb on - strand at 255.844 kb on - strand at 255.856 kb on - strand at 255.884 kb on - strand at 255.884 kb on - strand at 255.884 kb on - strand at 255.884 kb on - strand at 256.074 kb on - strand, within RR42_RS01095 at 256.394 kb on + strand at 256.407 kb on + strand at 256.407 kb on + strand at 256.407 kb on + strand at 256.408 kb on - strand at 256.480 kb on + strand at 256.480 kb on + strand at 256.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 253,346 + -0.2 253,346 + +0.5 253,346 + +0.6 253,346 + -0.2 253,347 - -0.0 253,347 - -2.6 253,347 - -0.1 253,425 - RR42_RS01080 0.31 +0.7 253,609 + +0.1 253,609 + +1.5 253,610 - -0.0 253,610 - -0.5 253,610 - -0.7 253,610 - -1.4 253,610 - -0.1 253,637 + -0.9 253,638 - +0.1 253,654 + +0.5 253,655 - +1.0 253,706 + -0.3 253,706 + +0.2 253,706 + +0.0 253,706 + -0.5 253,707 - -0.3 253,707 - -1.6 253,707 - -1.3 253,707 - -0.1 253,707 - -0.7 253,707 - -0.7 253,733 + RR42_RS01085 0.13 -0.2 253,733 + RR42_RS01085 0.13 -1.3 253,733 + RR42_RS01085 0.13 +0.3 253,733 + RR42_RS01085 0.13 -1.3 253,734 - RR42_RS01085 0.13 +0.7 253,894 + RR42_RS01085 0.39 -1.6 253,895 - RR42_RS01085 0.39 -1.0 253,895 - RR42_RS01085 0.39 -1.1 253,945 + RR42_RS01085 0.47 -1.2 253,945 + RR42_RS01085 0.47 -1.0 254,077 + RR42_RS01085 0.68 -1.1 254,077 + RR42_RS01085 0.68 +0.1 254,078 - RR42_RS01085 0.68 -0.6 254,433 + RR42_RS01090 0.11 +2.6 254,434 - RR42_RS01090 0.11 -1.0 254,754 + RR42_RS01090 0.34 -0.3 254,754 + RR42_RS01090 0.34 -0.5 254,754 + RR42_RS01090 0.34 +1.2 254,755 - RR42_RS01090 0.34 -1.5 254,755 - RR42_RS01090 0.34 -0.4 254,827 - RR42_RS01090 0.39 -1.5 254,874 + RR42_RS01090 0.43 -1.7 254,875 - RR42_RS01090 0.43 -0.5 254,875 - RR42_RS01090 0.43 -0.7 255,150 + RR42_RS01090 0.62 +0.7 255,150 + RR42_RS01090 0.62 -0.5 255,151 - RR42_RS01090 0.63 +0.2 255,151 - RR42_RS01090 0.63 +2.9 255,192 + RR42_RS01090 0.65 +0.7 255,192 + RR42_RS01090 0.65 -0.7 255,193 - RR42_RS01090 0.66 -0.2 255,193 - RR42_RS01090 0.66 -1.1 255,213 + RR42_RS01090 0.67 -0.4 255,213 + RR42_RS01090 0.67 +2.0 255,213 + RR42_RS01090 0.67 -0.3 255,213 + RR42_RS01090 0.67 -0.8 255,214 - RR42_RS01090 0.67 -0.2 255,229 - RR42_RS01090 0.68 +0.2 255,294 + RR42_RS01090 0.73 -0.9 255,295 - RR42_RS01090 0.73 -0.2 255,670 + +1.5 255,671 - +0.5 255,688 - -0.7 255,688 - +0.8 255,844 - -0.8 255,844 - -0.2 255,856 - -0.3 255,884 - -0.4 255,884 - +0.7 255,884 - -0.1 255,884 - +0.2 256,074 - RR42_RS01095 0.38 -1.0 256,394 + +0.9 256,407 + +0.1 256,407 + -0.6 256,407 + +0.4 256,408 - -1.8 256,480 + +0.1 256,480 + +1.3 256,481 - +0.6
Or see this region's nucleotide sequence