Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00825 and RR42_RS00830 are separated by 128 nucleotides RR42_RS00830 and RR42_RS00835 are separated by 91 nucleotides RR42_RS00835 and RR42_RS00840 are separated by 168 nucleotides
RR42_RS00825: RR42_RS00825 - arginyl-tRNA synthetase, at 201,494 to 203,287
_RS00825
RR42_RS00830: RR42_RS00830 - cell division protein FtsN, at 203,416 to 204,192
_RS00830
RR42_RS00835: RR42_RS00835 - DSBA oxidoreductase, at 204,284 to 204,922
_RS00835
RR42_RS00840: RR42_RS00840 - short-chain dehydrogenase, at 205,091 to 205,903
_RS00840
Position (kb) 203
204
205 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 203.285 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.335 kb on + strand at 203.335 kb on + strand at 203.341 kb on + strand at 203.386 kb on + strand at 203.387 kb on - strand at 203.388 kb on + strand at 203.476 kb on - strand at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.540 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.958 kb on - strand, within RR42_RS00830 at 203.958 kb on - strand, within RR42_RS00830 at 203.963 kb on - strand, within RR42_RS00830 at 204.082 kb on + strand, within RR42_RS00830 at 204.083 kb on - strand, within RR42_RS00830 at 204.180 kb on + strand at 204.181 kb on - strand at 204.191 kb on - strand at 204.191 kb on - strand at 204.200 kb on + strand at 204.250 kb on + strand at 204.250 kb on + strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.384 kb on - strand, within RR42_RS00835 at 204.450 kb on - strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.731 kb on + strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.732 kb on - strand, within RR42_RS00835 at 204.788 kb on + strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.789 kb on - strand, within RR42_RS00835 at 204.830 kb on + strand, within RR42_RS00835 at 204.830 kb on + strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.831 kb on - strand, within RR42_RS00835 at 204.921 kb on - strand at 204.921 kb on - strand at 205.157 kb on + strand at 205.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 203,285 + +0.7 203,285 + +0.6 203,285 + +1.4 203,285 + +0.5 203,286 - +1.0 203,286 - -0.3 203,286 - +1.1 203,286 - +0.5 203,335 + -0.3 203,335 + +0.9 203,341 + -0.1 203,386 + +0.1 203,387 - +0.8 203,388 + -1.6 203,476 - -0.3 203,539 + RR42_RS00830 0.16 +0.3 203,539 + RR42_RS00830 0.16 +0.3 203,539 + RR42_RS00830 0.16 -1.5 203,539 + RR42_RS00830 0.16 -4.0 203,540 - RR42_RS00830 0.16 -1.8 203,542 - RR42_RS00830 0.16 -3.3 203,542 - RR42_RS00830 0.16 -2.2 203,542 - RR42_RS00830 0.16 -0.7 203,957 + RR42_RS00830 0.70 +0.3 203,957 + RR42_RS00830 0.70 -1.6 203,957 + RR42_RS00830 0.70 -2.3 203,958 - RR42_RS00830 0.70 -1.8 203,958 - RR42_RS00830 0.70 -1.3 203,963 - RR42_RS00830 0.70 +0.7 204,082 + RR42_RS00830 0.86 -2.6 204,083 - RR42_RS00830 0.86 -1.7 204,180 + +1.2 204,181 - -0.7 204,191 - -1.1 204,191 - +2.3 204,200 + -1.1 204,250 + +0.5 204,250 + +0.2 204,251 - -0.7 204,251 - +0.6 204,251 - -0.6 204,251 - -0.3 204,251 - -0.7 204,251 - -2.3 204,251 - +0.3 204,251 - -0.0 204,384 - RR42_RS00835 0.16 +0.2 204,450 - RR42_RS00835 0.26 +0.3 204,731 + RR42_RS00835 0.70 -1.0 204,731 + RR42_RS00835 0.70 -1.8 204,731 + RR42_RS00835 0.70 +0.5 204,732 - RR42_RS00835 0.70 -1.2 204,732 - RR42_RS00835 0.70 -0.4 204,732 - RR42_RS00835 0.70 -0.3 204,732 - RR42_RS00835 0.70 +0.3 204,732 - RR42_RS00835 0.70 +0.4 204,732 - RR42_RS00835 0.70 -1.8 204,732 - RR42_RS00835 0.70 +0.3 204,732 - RR42_RS00835 0.70 +0.4 204,788 + RR42_RS00835 0.79 -0.5 204,789 - RR42_RS00835 0.79 -0.7 204,789 - RR42_RS00835 0.79 +0.4 204,789 - RR42_RS00835 0.79 -0.5 204,830 + RR42_RS00835 0.85 -1.7 204,830 + RR42_RS00835 0.85 +0.5 204,831 - RR42_RS00835 0.86 +0.7 204,831 - RR42_RS00835 0.86 +1.3 204,831 - RR42_RS00835 0.86 +0.8 204,831 - RR42_RS00835 0.86 +0.7 204,831 - RR42_RS00835 0.86 -0.1 204,831 - RR42_RS00835 0.86 -0.3 204,921 - +0.3 204,921 - +0.6 205,157 + +0.6 205,158 - -0.2
Or see this region's nucleotide sequence