Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00815 and RR42_RS00820 are separated by 313 nucleotides RR42_RS00820 and RR42_RS00825 are separated by 260 nucleotides RR42_RS00825 and RR42_RS00830 are separated by 128 nucleotides RR42_RS00830 and RR42_RS00835 are separated by 91 nucleotides
RR42_RS00815: RR42_RS00815 - hypothetical protein, at 200,180 to 200,596
_RS00815
RR42_RS00820: RR42_RS00820 - hypothetical protein, at 200,910 to 201,233
_RS00820
RR42_RS00825: RR42_RS00825 - arginyl-tRNA synthetase, at 201,494 to 203,287
_RS00825
RR42_RS00830: RR42_RS00830 - cell division protein FtsN, at 203,416 to 204,192
_RS00830
RR42_RS00835: RR42_RS00835 - DSBA oxidoreductase, at 204,284 to 204,922
_RS00835
Position (kb) 201
202
203
204 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 200.527 kb on + strand, within RR42_RS00815 at 200.527 kb on + strand, within RR42_RS00815 at 200.527 kb on + strand, within RR42_RS00815 at 200.648 kb on + strand at 200.672 kb on - strand at 200.839 kb on - strand at 200.865 kb on + strand at 200.876 kb on + strand at 200.881 kb on + strand at 200.881 kb on + strand at 200.881 kb on + strand at 200.882 kb on - strand at 200.889 kb on + strand at 200.911 kb on + strand at 200.912 kb on - strand at 200.912 kb on - strand at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.093 kb on - strand, within RR42_RS00820 at 201.237 kb on - strand at 201.237 kb on - strand at 201.237 kb on - strand at 201.237 kb on - strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.265 kb on - strand at 201.265 kb on - strand at 201.265 kb on - strand at 201.367 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.335 kb on + strand at 203.335 kb on + strand at 203.341 kb on + strand at 203.386 kb on + strand at 203.387 kb on - strand at 203.388 kb on + strand at 203.476 kb on - strand at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.540 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.958 kb on - strand, within RR42_RS00830 at 203.958 kb on - strand, within RR42_RS00830 at 203.963 kb on - strand, within RR42_RS00830 at 204.082 kb on + strand, within RR42_RS00830 at 204.083 kb on - strand, within RR42_RS00830 at 204.180 kb on + strand at 204.181 kb on - strand at 204.191 kb on - strand at 204.191 kb on - strand at 204.200 kb on + strand at 204.250 kb on + strand at 204.250 kb on + strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 200,527 + RR42_RS00815 0.83 +0.1 200,527 + RR42_RS00815 0.83 +0.3 200,527 + RR42_RS00815 0.83 +0.2 200,648 + +0.6 200,672 - +1.0 200,839 - -0.2 200,865 + +0.5 200,876 + +0.9 200,881 + +1.5 200,881 + -1.6 200,881 + -2.5 200,882 - -0.1 200,889 + -0.3 200,911 + -0.4 200,912 - -0.7 200,912 - +1.2 201,090 + RR42_RS00820 0.56 -0.7 201,090 + RR42_RS00820 0.56 +1.3 201,090 + RR42_RS00820 0.56 +0.5 201,090 + RR42_RS00820 0.56 -1.7 201,090 + RR42_RS00820 0.56 +1.2 201,090 + RR42_RS00820 0.56 +0.5 201,091 - RR42_RS00820 0.56 -0.8 201,091 - RR42_RS00820 0.56 -1.7 201,091 - RR42_RS00820 0.56 -1.2 201,091 - RR42_RS00820 0.56 -0.3 201,091 - RR42_RS00820 0.56 -0.8 201,093 - RR42_RS00820 0.56 +0.4 201,237 - -0.0 201,237 - +0.4 201,237 - +0.8 201,237 - +1.0 201,264 + -0.4 201,264 + +0.4 201,264 + +0.4 201,264 + +0.4 201,264 + +0.8 201,264 + +0.1 201,265 - +1.5 201,265 - +0.6 201,265 - +0.5 201,367 + -0.9 203,285 + +0.7 203,285 + +0.6 203,285 + +1.4 203,285 + +0.5 203,286 - +1.0 203,286 - -0.3 203,286 - +1.1 203,286 - +0.5 203,335 + -0.3 203,335 + +0.9 203,341 + -0.1 203,386 + +0.1 203,387 - +0.8 203,388 + -1.6 203,476 - -0.3 203,539 + RR42_RS00830 0.16 +0.3 203,539 + RR42_RS00830 0.16 +0.3 203,539 + RR42_RS00830 0.16 -1.5 203,539 + RR42_RS00830 0.16 -4.0 203,540 - RR42_RS00830 0.16 -1.8 203,542 - RR42_RS00830 0.16 -3.3 203,542 - RR42_RS00830 0.16 -2.2 203,542 - RR42_RS00830 0.16 -0.7 203,957 + RR42_RS00830 0.70 +0.3 203,957 + RR42_RS00830 0.70 -1.6 203,957 + RR42_RS00830 0.70 -2.3 203,958 - RR42_RS00830 0.70 -1.8 203,958 - RR42_RS00830 0.70 -1.3 203,963 - RR42_RS00830 0.70 +0.7 204,082 + RR42_RS00830 0.86 -2.6 204,083 - RR42_RS00830 0.86 -1.7 204,180 + +1.2 204,181 - -0.7 204,191 - -1.1 204,191 - +2.3 204,200 + -1.1 204,250 + +0.5 204,250 + +0.2 204,251 - -0.7 204,251 - +0.6 204,251 - -0.6 204,251 - -0.3 204,251 - -0.7 204,251 - -2.3 204,251 - +0.3 204,251 - -0.0
Or see this region's nucleotide sequence