Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00715 and RR42_RS00720 are separated by 219 nucleotides RR42_RS00720 and RR42_RS00725 are separated by 69 nucleotides
RR42_RS00715: RR42_RS00715 - ferritin, at 180,744 to 181,607
_RS00715
RR42_RS00720: RR42_RS00720 - patatin, at 181,827 to 183,068
_RS00720
RR42_RS00725: RR42_RS00725 - hypothetical protein, at 183,138 to 184,046
_RS00725
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 180.858 kb on - strand, within RR42_RS00715 at 180.858 kb on - strand, within RR42_RS00715 at 181.103 kb on - strand, within RR42_RS00715 at 181.166 kb on - strand, within RR42_RS00715 at 181.611 kb on - strand at 181.721 kb on - strand at 181.773 kb on - strand at 181.773 kb on - strand at 181.773 kb on - strand at 181.773 kb on - strand at 181.824 kb on - strand at 181.866 kb on - strand at 181.866 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 181.925 kb on - strand at 182.018 kb on - strand, within RR42_RS00720 at 182.018 kb on - strand, within RR42_RS00720 at 182.584 kb on + strand, within RR42_RS00720 at 182.585 kb on - strand, within RR42_RS00720 at 182.585 kb on - strand, within RR42_RS00720 at 182.610 kb on - strand, within RR42_RS00720 at 182.610 kb on - strand, within RR42_RS00720 at 182.612 kb on - strand, within RR42_RS00720 at 182.612 kb on - strand, within RR42_RS00720 at 182.612 kb on - strand, within RR42_RS00720 at 183.006 kb on + strand at 183.007 kb on - strand at 183.159 kb on - strand at 183.450 kb on - strand, within RR42_RS00725 at 183.657 kb on - strand, within RR42_RS00725 at 183.657 kb on - strand, within RR42_RS00725 at 183.706 kb on + strand, within RR42_RS00725 at 183.706 kb on + strand, within RR42_RS00725 at 183.707 kb on - strand, within RR42_RS00725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 180,858 - RR42_RS00715 0.13 +2.7 180,858 - RR42_RS00715 0.13 +3.1 181,103 - RR42_RS00715 0.42 +2.5 181,166 - RR42_RS00715 0.49 +3.2 181,611 - +0.6 181,721 - +0.1 181,773 - -1.6 181,773 - -0.2 181,773 - +0.7 181,773 - -0.7 181,824 - -1.4 181,866 - -0.8 181,866 - -0.8 181,925 - -1.3 181,925 - -1.1 181,925 - -0.3 181,925 - -2.2 181,925 - -0.4 181,925 - -1.3 181,925 - +0.7 182,018 - RR42_RS00720 0.15 +0.1 182,018 - RR42_RS00720 0.15 +1.0 182,584 + RR42_RS00720 0.61 +0.7 182,585 - RR42_RS00720 0.61 -0.2 182,585 - RR42_RS00720 0.61 +0.2 182,610 - RR42_RS00720 0.63 -0.9 182,610 - RR42_RS00720 0.63 +0.2 182,612 - RR42_RS00720 0.63 -0.1 182,612 - RR42_RS00720 0.63 -1.0 182,612 - RR42_RS00720 0.63 -0.7 183,006 + -0.0 183,007 - +0.2 183,159 - -2.9 183,450 - RR42_RS00725 0.34 -0.0 183,657 - RR42_RS00725 0.57 -0.2 183,657 - RR42_RS00725 0.57 +1.3 183,706 + RR42_RS00725 0.62 +1.2 183,706 + RR42_RS00725 0.62 +0.1 183,707 - RR42_RS00725 0.63 +0.7
Or see this region's nucleotide sequence