Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00435 and RR42_RS00440 are separated by 83 nucleotides RR42_RS00440 and RR42_RS00445 are separated by 81 nucleotides RR42_RS00445 and RR42_RS00450 are separated by 351 nucleotides RR42_RS00450 and RR42_RS00455 are separated by 15 nucleotides
RR42_RS00435: RR42_RS00435 - transcriptional regulator, at 113,967 to 114,665
_RS00435
RR42_RS00440: RR42_RS00440 - hypothetical protein, at 114,749 to 115,528
_RS00440
RR42_RS00445: RR42_RS00445 - membrane protein, at 115,610 to 116,698
_RS00445
RR42_RS00450: RR42_RS00450 - cell division topological specificity factor MinE, at 117,050 to 117,304
_RS00450
RR42_RS00455: RR42_RS00455 - cell division inhibitor MinD, at 117,320 to 118,135
_RS00455
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 114.621 kb on + strand at 115.004 kb on + strand, within RR42_RS00440 at 115.084 kb on - strand, within RR42_RS00440 at 115.699 kb on - strand at 115.699 kb on - strand at 115.699 kb on - strand at 115.699 kb on - strand at 115.819 kb on - strand, within RR42_RS00445 at 115.819 kb on - strand, within RR42_RS00445 at 115.858 kb on - strand, within RR42_RS00445 at 115.858 kb on - strand, within RR42_RS00445 at 115.858 kb on - strand, within RR42_RS00445 at 115.858 kb on - strand, within RR42_RS00445 at 115.989 kb on + strand, within RR42_RS00445 at 115.989 kb on + strand, within RR42_RS00445 at 115.990 kb on - strand, within RR42_RS00445 at 116.001 kb on - strand, within RR42_RS00445 at 116.001 kb on - strand, within RR42_RS00445 at 116.022 kb on + strand, within RR42_RS00445 at 116.022 kb on + strand, within RR42_RS00445 at 116.022 kb on + strand, within RR42_RS00445 at 116.023 kb on - strand, within RR42_RS00445 at 116.023 kb on - strand, within RR42_RS00445 at 116.083 kb on - strand, within RR42_RS00445 at 116.113 kb on + strand, within RR42_RS00445 at 116.113 kb on + strand, within RR42_RS00445 at 116.113 kb on + strand, within RR42_RS00445 at 116.114 kb on - strand, within RR42_RS00445 at 116.114 kb on - strand, within RR42_RS00445 at 116.114 kb on - strand, within RR42_RS00445 at 116.115 kb on + strand, within RR42_RS00445 at 116.116 kb on - strand, within RR42_RS00445 at 116.137 kb on - strand, within RR42_RS00445 at 116.193 kb on + strand, within RR42_RS00445 at 116.193 kb on + strand, within RR42_RS00445 at 116.194 kb on - strand, within RR42_RS00445 at 116.194 kb on - strand, within RR42_RS00445 at 116.194 kb on - strand, within RR42_RS00445 at 116.194 kb on - strand, within RR42_RS00445 at 116.232 kb on + strand, within RR42_RS00445 at 116.232 kb on + strand, within RR42_RS00445 at 116.233 kb on - strand, within RR42_RS00445 at 116.292 kb on + strand, within RR42_RS00445 at 116.292 kb on + strand, within RR42_RS00445 at 116.293 kb on - strand, within RR42_RS00445 at 116.297 kb on - strand, within RR42_RS00445 at 116.297 kb on - strand, within RR42_RS00445 at 116.297 kb on - strand, within RR42_RS00445 at 116.297 kb on - strand, within RR42_RS00445 at 116.297 kb on - strand, within RR42_RS00445 at 116.346 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.394 kb on + strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.395 kb on - strand, within RR42_RS00445 at 116.451 kb on + strand, within RR42_RS00445 at 116.451 kb on + strand, within RR42_RS00445 at 116.611 kb on + strand at 116.616 kb on + strand at 116.616 kb on + strand at 116.616 kb on + strand at 116.617 kb on - strand at 116.643 kb on + strand at 116.665 kb on + strand at 116.666 kb on - strand at 116.666 kb on - strand at 116.712 kb on + strand at 116.712 kb on + strand at 116.713 kb on - strand at 116.756 kb on - strand at 116.767 kb on - strand at 116.810 kb on + strand at 116.810 kb on + strand at 116.810 kb on + strand at 116.811 kb on - strand at 116.811 kb on - strand at 116.811 kb on - strand at 116.811 kb on - strand at 116.833 kb on + strand at 116.834 kb on - strand at 116.912 kb on + strand at 116.921 kb on - strand at 116.938 kb on + strand at 117.322 kb on - strand at 117.322 kb on - strand at 117.363 kb on + strand at 117.420 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.588 kb on + strand, within RR42_RS00455 at 117.589 kb on - strand, within RR42_RS00455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 114,621 + +0.6 115,004 + RR42_RS00440 0.33 -2.1 115,084 - RR42_RS00440 0.43 +1.4 115,699 - -1.7 115,699 - -3.1 115,699 - -2.5 115,699 - -0.3 115,819 - RR42_RS00445 0.19 -1.0 115,819 - RR42_RS00445 0.19 -4.3 115,858 - RR42_RS00445 0.23 -3.4 115,858 - RR42_RS00445 0.23 -3.1 115,858 - RR42_RS00445 0.23 -1.3 115,858 - RR42_RS00445 0.23 -2.9 115,989 + RR42_RS00445 0.35 -3.6 115,989 + RR42_RS00445 0.35 -3.3 115,990 - RR42_RS00445 0.35 -2.1 116,001 - RR42_RS00445 0.36 -3.1 116,001 - RR42_RS00445 0.36 -2.6 116,022 + RR42_RS00445 0.38 -1.5 116,022 + RR42_RS00445 0.38 +0.1 116,022 + RR42_RS00445 0.38 -1.0 116,023 - RR42_RS00445 0.38 -4.4 116,023 - RR42_RS00445 0.38 -4.0 116,083 - RR42_RS00445 0.43 -4.4 116,113 + RR42_RS00445 0.46 -2.1 116,113 + RR42_RS00445 0.46 -0.3 116,113 + RR42_RS00445 0.46 -1.3 116,114 - RR42_RS00445 0.46 -3.3 116,114 - RR42_RS00445 0.46 -2.3 116,114 - RR42_RS00445 0.46 -2.4 116,115 + RR42_RS00445 0.46 -1.0 116,116 - RR42_RS00445 0.46 -0.0 116,137 - RR42_RS00445 0.48 -1.3 116,193 + RR42_RS00445 0.54 -1.8 116,193 + RR42_RS00445 0.54 -2.5 116,194 - RR42_RS00445 0.54 -3.6 116,194 - RR42_RS00445 0.54 -2.1 116,194 - RR42_RS00445 0.54 -2.7 116,194 - RR42_RS00445 0.54 -5.6 116,232 + RR42_RS00445 0.57 -1.4 116,232 + RR42_RS00445 0.57 -2.6 116,233 - RR42_RS00445 0.57 -2.0 116,292 + RR42_RS00445 0.63 -2.0 116,292 + RR42_RS00445 0.63 -3.9 116,293 - RR42_RS00445 0.63 -1.3 116,297 - RR42_RS00445 0.63 -2.0 116,297 - RR42_RS00445 0.63 -1.5 116,297 - RR42_RS00445 0.63 -1.0 116,297 - RR42_RS00445 0.63 -2.8 116,297 - RR42_RS00445 0.63 -1.8 116,346 + RR42_RS00445 0.68 -3.7 116,394 + RR42_RS00445 0.72 -4.4 116,394 + RR42_RS00445 0.72 -2.8 116,394 + RR42_RS00445 0.72 -3.3 116,394 + RR42_RS00445 0.72 +0.3 116,394 + RR42_RS00445 0.72 -2.0 116,394 + RR42_RS00445 0.72 -5.3 116,394 + RR42_RS00445 0.72 -4.1 116,394 + RR42_RS00445 0.72 -2.0 116,395 - RR42_RS00445 0.72 -2.5 116,395 - RR42_RS00445 0.72 -3.7 116,395 - RR42_RS00445 0.72 -3.9 116,395 - RR42_RS00445 0.72 -0.8 116,395 - RR42_RS00445 0.72 -3.0 116,395 - RR42_RS00445 0.72 -1.2 116,395 - RR42_RS00445 0.72 -2.3 116,395 - RR42_RS00445 0.72 -3.4 116,451 + RR42_RS00445 0.77 -5.2 116,451 + RR42_RS00445 0.77 -1.3 116,611 + -3.0 116,616 + -2.0 116,616 + -0.7 116,616 + -2.4 116,617 - -2.6 116,643 + -1.4 116,665 + -2.6 116,666 - -3.9 116,666 - -3.1 116,712 + -2.0 116,712 + -3.1 116,713 - -1.3 116,756 - +3.6 116,767 - -2.5 116,810 + -1.5 116,810 + -3.4 116,810 + +0.3 116,811 - -1.2 116,811 - -2.4 116,811 - -2.6 116,811 - -0.5 116,833 + -2.4 116,834 - -2.9 116,912 + -2.4 116,921 - +1.7 116,938 + -2.6 117,322 - +0.2 117,322 - -0.1 117,363 + -2.5 117,420 + RR42_RS00455 0.12 -0.0 117,588 + RR42_RS00455 0.33 +1.7 117,588 + RR42_RS00455 0.33 +0.3 117,588 + RR42_RS00455 0.33 -2.9 117,588 + RR42_RS00455 0.33 +0.7 117,589 - RR42_RS00455 0.33 +1.3
Or see this region's nucleotide sequence