Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_76 and Pf1N1B4_77 are separated by 307 nucleotides Pf1N1B4_77 and Pf1N1B4_78 are separated by 143 nucleotides Pf1N1B4_78 and Pf1N1B4_79 are separated by 179 nucleotides
Pf1N1B4_76 - ATP binding protein, at 79,325 to 80,692
_76
Pf1N1B4_77 - FIG00956064: hypothetical protein, at 81,000 to 81,317
_77
Pf1N1B4_78 - FIG00956937: hypothetical protein, at 81,461 to 81,994
_78
Pf1N1B4_79 - Lactoylglutathione lyase and related lyases, at 82,174 to 82,542
_79
Position (kb) 80
81
82 Strain fitness (log2 ratio) -2
-1
0
1
2 at 80.281 kb on - strand, within Pf1N1B4_76 at 80.281 kb on - strand, within Pf1N1B4_76 at 80.344 kb on - strand, within Pf1N1B4_76 at 80.374 kb on - strand, within Pf1N1B4_76 at 80.449 kb on - strand, within Pf1N1B4_76 at 80.503 kb on - strand, within Pf1N1B4_76 at 80.659 kb on - strand at 80.705 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.752 kb on + strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.753 kb on - strand at 80.824 kb on + strand at 80.824 kb on + strand at 80.824 kb on + strand at 80.825 kb on - strand at 80.825 kb on - strand at 80.877 kb on - strand at 80.877 kb on - strand at 80.911 kb on + strand at 80.927 kb on - strand at 81.030 kb on + strand at 81.030 kb on + strand at 81.031 kb on - strand at 81.071 kb on + strand, within Pf1N1B4_77 at 81.071 kb on + strand, within Pf1N1B4_77 at 81.071 kb on + strand, within Pf1N1B4_77 at 81.137 kb on + strand, within Pf1N1B4_77 at 81.162 kb on + strand, within Pf1N1B4_77 at 81.220 kb on + strand, within Pf1N1B4_77 at 81.289 kb on + strand at 81.290 kb on - strand at 81.290 kb on - strand at 81.315 kb on + strand at 81.316 kb on - strand at 81.334 kb on + strand at 81.335 kb on - strand at 81.335 kb on - strand at 81.403 kb on - strand at 81.403 kb on - strand at 81.426 kb on + strand at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.778 kb on - strand, within Pf1N1B4_78 at 81.967 kb on + strand at 81.968 kb on - strand at 82.022 kb on + strand at 82.064 kb on + strand at 82.064 kb on + strand at 82.064 kb on + strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.065 kb on - strand at 82.070 kb on + strand at 82.071 kb on - strand at 82.071 kb on - strand at 82.071 kb on - strand at 82.090 kb on + strand at 82.090 kb on + strand at 82.091 kb on - strand at 82.091 kb on - strand at 82.091 kb on - strand at 82.124 kb on + strand at 82.125 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 80,281 - Pf1N1B4_76 0.70 -1.6 80,281 - Pf1N1B4_76 0.70 -0.2 80,344 - Pf1N1B4_76 0.74 +0.0 80,374 - Pf1N1B4_76 0.77 -0.6 80,449 - Pf1N1B4_76 0.82 -2.6 80,503 - Pf1N1B4_76 0.86 -0.3 80,659 - -0.3 80,705 + +0.4 80,752 + +0.0 80,752 + +1.3 80,752 + -2.0 80,752 + -0.8 80,753 - +1.2 80,753 - -0.6 80,753 - -0.1 80,753 - +0.3 80,824 + -0.6 80,824 + +1.0 80,824 + -0.1 80,825 - -0.2 80,825 - -0.6 80,877 - -1.4 80,877 - +1.6 80,911 + -0.3 80,927 - +2.0 81,030 + -0.4 81,030 + -2.7 81,031 - -0.2 81,071 + Pf1N1B4_77 0.22 -0.8 81,071 + Pf1N1B4_77 0.22 +0.8 81,071 + Pf1N1B4_77 0.22 -0.4 81,137 + Pf1N1B4_77 0.43 -1.0 81,162 + Pf1N1B4_77 0.51 -0.7 81,220 + Pf1N1B4_77 0.69 -0.7 81,289 + +0.3 81,290 - -1.6 81,290 - +0.0 81,315 + +0.3 81,316 - -0.2 81,334 + -0.2 81,335 - -1.4 81,335 - -0.1 81,403 - -0.3 81,403 - -0.6 81,426 + +1.2 81,778 - Pf1N1B4_78 0.59 +0.4 81,778 - Pf1N1B4_78 0.59 +0.0 81,778 - Pf1N1B4_78 0.59 +0.2 81,778 - Pf1N1B4_78 0.59 +0.8 81,967 + +0.7 81,968 - -0.1 82,022 + +0.5 82,064 + -1.0 82,064 + -0.1 82,064 + +0.7 82,065 - -0.5 82,065 - -1.6 82,065 - +0.0 82,065 - +1.0 82,065 - -1.2 82,070 + +1.0 82,071 - +0.6 82,071 - +1.0 82,071 - +0.4 82,090 + -0.2 82,090 + +0.2 82,091 - -0.8 82,091 - +0.4 82,091 - -0.1 82,124 + +0.4 82,125 - -0.6
Or see this region's nucleotide sequence