Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_75

Experiment: a-Ketoglutaric (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_73 and Pf1N1B4_74 are separated by 231 nucleotidesPf1N1B4_74 and Pf1N1B4_75 are separated by 16 nucleotidesPf1N1B4_75 and Pf1N1B4_76 are separated by 501 nucleotides Pf1N1B4_73 - Beta-lactamase (EC 3.5.2.6), at 76,762 to 77,934 _73 Pf1N1B4_74 - hypothetical protein, at 78,166 to 78,429 _74 Pf1N1B4_75 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 78,446 to 78,823 _75 Pf1N1B4_76 - ATP binding protein, at 79,325 to 80,692 _76 Position (kb) 78 79Strain fitness (log2 ratio) -2 -1 0 1 2at 77.452 kb on + strand, within Pf1N1B4_73at 77.620 kb on + strandat 77.623 kb on + strand, within Pf1N1B4_73at 77.623 kb on + strand, within Pf1N1B4_73at 77.623 kb on + strand, within Pf1N1B4_73at 77.623 kb on + strand, within Pf1N1B4_73at 77.623 kb on + strand, within Pf1N1B4_73at 77.623 kb on + strand, within Pf1N1B4_73at 77.624 kb on - strand, within Pf1N1B4_73at 77.624 kb on - strand, within Pf1N1B4_73at 77.624 kb on - strand, within Pf1N1B4_73at 77.624 kb on - strand, within Pf1N1B4_73at 77.665 kb on + strand, within Pf1N1B4_73at 77.665 kb on + strand, within Pf1N1B4_73at 77.665 kb on + strand, within Pf1N1B4_73at 77.665 kb on + strand, within Pf1N1B4_73at 77.665 kb on + strand, within Pf1N1B4_73at 77.665 kb on + strand, within Pf1N1B4_73at 77.666 kb on - strand, within Pf1N1B4_73at 77.666 kb on - strand, within Pf1N1B4_73at 77.666 kb on - strand, within Pf1N1B4_73at 77.666 kb on - strand, within Pf1N1B4_73at 77.672 kb on - strand, within Pf1N1B4_73at 77.688 kb on + strand, within Pf1N1B4_73at 77.688 kb on + strand, within Pf1N1B4_73at 77.688 kb on + strand, within Pf1N1B4_73at 77.689 kb on - strand, within Pf1N1B4_73at 77.689 kb on - strand, within Pf1N1B4_73at 77.819 kb on - strandat 77.876 kb on - strandat 77.912 kb on + strandat 77.912 kb on + strandat 77.912 kb on + strandat 77.912 kb on + strandat 77.913 kb on - strandat 77.913 kb on - strandat 78.032 kb on + strandat 78.033 kb on - strandat 78.033 kb on - strandat 78.033 kb on - strandat 78.040 kb on - strandat 78.060 kb on - strandat 78.121 kb on + strandat 78.122 kb on - strandat 78.139 kb on - strandat 78.139 kb on - strandat 78.167 kb on + strandat 78.168 kb on - strandat 78.168 kb on - strandat 78.205 kb on + strand, within Pf1N1B4_74at 78.206 kb on - strand, within Pf1N1B4_74at 78.206 kb on - strand, within Pf1N1B4_74at 78.304 kb on + strand, within Pf1N1B4_74at 78.305 kb on - strand, within Pf1N1B4_74at 78.419 kb on + strandat 78.419 kb on + strandat 78.419 kb on + strandat 78.419 kb on + strandat 78.420 kb on - strandat 78.420 kb on - strandat 78.420 kb on - strandat 78.420 kb on - strandat 78.420 kb on - strandat 78.685 kb on + strand, within Pf1N1B4_75at 78.685 kb on + strand, within Pf1N1B4_75at 78.686 kb on - strand, within Pf1N1B4_75at 78.813 kb on - strandat 78.844 kb on + strandat 78.888 kb on + strandat 79.002 kb on + strandat 79.003 kb on - strandat 79.181 kb on + strandat 79.181 kb on + strandat 79.182 kb on - strandat 79.182 kb on - strandat 79.182 kb on - strandat 79.182 kb on - strandat 79.200 kb on + strandat 79.201 kb on - strandat 79.236 kb on - strandat 79.276 kb on + strandat 79.276 kb on + strandat 79.277 kb on - strandat 79.277 kb on - strandat 79.324 kb on - strandat 79.587 kb on + strandat 79.785 kb on - strand, within Pf1N1B4_76

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Per-strain Table

Position Strand Gene LocusTag Fraction a-Ketoglutaric (C)
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77,452 + Pf1N1B4_73 0.59 -1.2
77,620 + +0.4
77,623 + Pf1N1B4_73 0.73 -1.0
77,623 + Pf1N1B4_73 0.73 -0.2
77,623 + Pf1N1B4_73 0.73 +1.3
77,623 + Pf1N1B4_73 0.73 +1.0
77,623 + Pf1N1B4_73 0.73 -2.2
77,623 + Pf1N1B4_73 0.73 +0.0
77,624 - Pf1N1B4_73 0.73 +0.7
77,624 - Pf1N1B4_73 0.73 -0.5
77,624 - Pf1N1B4_73 0.73 -0.6
77,624 - Pf1N1B4_73 0.73 +2.6
77,665 + Pf1N1B4_73 0.77 +0.0
77,665 + Pf1N1B4_73 0.77 +1.0
77,665 + Pf1N1B4_73 0.77 +1.0
77,665 + Pf1N1B4_73 0.77 +0.2
77,665 + Pf1N1B4_73 0.77 -0.4
77,665 + Pf1N1B4_73 0.77 -0.9
77,666 - Pf1N1B4_73 0.77 +2.0
77,666 - Pf1N1B4_73 0.77 +1.0
77,666 - Pf1N1B4_73 0.77 +0.0
77,666 - Pf1N1B4_73 0.77 -0.8
77,672 - Pf1N1B4_73 0.78 +0.0
77,688 + Pf1N1B4_73 0.79 +0.4
77,688 + Pf1N1B4_73 0.79 +1.0
77,688 + Pf1N1B4_73 0.79 -1.8
77,689 - Pf1N1B4_73 0.79 +1.0
77,689 - Pf1N1B4_73 0.79 -0.7
77,819 - -0.0
77,876 - +1.0
77,912 + +0.4
77,912 + +1.8
77,912 + +1.2
77,912 + +0.2
77,913 - -0.1
77,913 - +0.7
78,032 + -0.8
78,033 - +1.0
78,033 - -1.3
78,033 - -1.0
78,040 - -0.6
78,060 - -0.8
78,121 + +1.3
78,122 - +0.5
78,139 - -0.8
78,139 - +0.3
78,167 + +0.7
78,168 - +2.0
78,168 - -0.3
78,205 + Pf1N1B4_74 0.15 -0.2
78,206 - Pf1N1B4_74 0.15 +0.0
78,206 - Pf1N1B4_74 0.15 -0.2
78,304 + Pf1N1B4_74 0.52 +0.2
78,305 - Pf1N1B4_74 0.53 -0.6
78,419 + -0.1
78,419 + +0.8
78,419 + +0.4
78,419 + -0.3
78,420 - -0.4
78,420 - +0.0
78,420 - -0.8
78,420 - +0.0
78,420 - -0.5
78,685 + Pf1N1B4_75 0.63 -1.3
78,685 + Pf1N1B4_75 0.63 +0.0
78,686 - Pf1N1B4_75 0.63 +0.0
78,813 - -0.6
78,844 + -0.2
78,888 + -1.3
79,002 + +0.0
79,003 - -1.6
79,181 + +1.6
79,181 + -0.1
79,182 - +1.0
79,182 - +0.5
79,182 - -1.6
79,182 - +0.0
79,200 + +0.0
79,201 - -0.8
79,236 - +1.0
79,276 + -0.2
79,276 + +1.0
79,277 - -0.8
79,277 - +0.1
79,324 - +0.0
79,587 + +0.0
79,785 - Pf1N1B4_76 0.34 -1.0

Or see this region's nucleotide sequence