Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_71 and Pf1N1B4_72 are separated by 123 nucleotides Pf1N1B4_72 and Pf1N1B4_73 are separated by 142 nucleotides
Pf1N1B4_71 - Glucarate dehydratase (EC 4.2.1.40), at 74,280 to 75,623
_71
Pf1N1B4_72 - Transcriptional regulator, LysR family, at 75,747 to 76,619
_72
Pf1N1B4_73 - Beta-lactamase (EC 3.5.2.6), at 76,762 to 77,934
_73
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-2
-1
0
1 at 75.206 kb on - strand, within Pf1N1B4_71 at 75.210 kb on - strand, within Pf1N1B4_71 at 75.320 kb on + strand, within Pf1N1B4_71 at 75.523 kb on - strand at 75.523 kb on - strand at 75.523 kb on - strand at 75.523 kb on - strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.745 kb on + strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.746 kb on - strand at 75.749 kb on - strand at 75.749 kb on - strand at 75.829 kb on + strand at 76.251 kb on + strand, within Pf1N1B4_72 at 76.251 kb on + strand, within Pf1N1B4_72 at 76.252 kb on - strand, within Pf1N1B4_72 at 76.252 kb on - strand, within Pf1N1B4_72 at 76.656 kb on + strand at 76.657 kb on - strand at 76.688 kb on + strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.689 kb on - strand at 76.786 kb on - strand at 76.813 kb on + strand at 76.814 kb on - strand at 76.973 kb on - strand, within Pf1N1B4_73 at 76.973 kb on - strand, within Pf1N1B4_73 at 77.071 kb on + strand, within Pf1N1B4_73 at 77.124 kb on - strand, within Pf1N1B4_73 at 77.132 kb on + strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.133 kb on - strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.179 kb on + strand, within Pf1N1B4_73 at 77.180 kb on - strand, within Pf1N1B4_73 at 77.180 kb on - strand, within Pf1N1B4_73 at 77.245 kb on + strand, within Pf1N1B4_73 at 77.246 kb on - strand, within Pf1N1B4_73 at 77.293 kb on + strand, within Pf1N1B4_73 at 77.407 kb on + strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.408 kb on - strand, within Pf1N1B4_73 at 77.440 kb on + strand, within Pf1N1B4_73 at 77.452 kb on + strand, within Pf1N1B4_73
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 75,206 - Pf1N1B4_71 0.69 +1.6 75,210 - Pf1N1B4_71 0.69 -0.8 75,320 + Pf1N1B4_71 0.77 -1.0 75,523 - +0.4 75,523 - +0.0 75,523 - +0.0 75,523 - +0.2 75,745 + -2.0 75,745 + +1.0 75,745 + +0.0 75,745 + +1.6 75,745 + +0.0 75,746 - +0.3 75,746 - +0.0 75,746 - -0.3 75,746 - -0.6 75,749 - +1.2 75,749 - -1.2 75,829 + -0.4 76,251 + Pf1N1B4_72 0.58 +1.0 76,251 + Pf1N1B4_72 0.58 +1.3 76,252 - Pf1N1B4_72 0.58 +1.2 76,252 - Pf1N1B4_72 0.58 -0.6 76,656 + -0.8 76,657 - +1.0 76,688 + -0.6 76,689 - -0.2 76,689 - +0.4 76,689 - -0.1 76,689 - +1.4 76,786 - +1.0 76,813 + -0.1 76,814 - -0.6 76,973 - Pf1N1B4_73 0.18 -0.1 76,973 - Pf1N1B4_73 0.18 -1.4 77,071 + Pf1N1B4_73 0.26 +0.5 77,124 - Pf1N1B4_73 0.31 -1.2 77,132 + Pf1N1B4_73 0.32 +0.0 77,133 - Pf1N1B4_73 0.32 -0.7 77,133 - Pf1N1B4_73 0.32 +0.1 77,133 - Pf1N1B4_73 0.32 -1.0 77,133 - Pf1N1B4_73 0.32 +0.3 77,179 + Pf1N1B4_73 0.36 -0.6 77,179 + Pf1N1B4_73 0.36 +0.3 77,179 + Pf1N1B4_73 0.36 +1.0 77,180 - Pf1N1B4_73 0.36 -2.0 77,180 - Pf1N1B4_73 0.36 +0.3 77,245 + Pf1N1B4_73 0.41 -0.7 77,246 - Pf1N1B4_73 0.41 +0.0 77,293 + Pf1N1B4_73 0.45 -1.3 77,407 + Pf1N1B4_73 0.55 -2.3 77,408 - Pf1N1B4_73 0.55 +1.0 77,408 - Pf1N1B4_73 0.55 +0.4 77,408 - Pf1N1B4_73 0.55 -1.3 77,440 + Pf1N1B4_73 0.58 -0.2 77,452 + Pf1N1B4_73 0.59 -1.2
Or see this region's nucleotide sequence