Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_685 and Pf1N1B4_686 are separated by 292 nucleotides Pf1N1B4_686 and Pf1N1B4_687 are separated by 241 nucleotides Pf1N1B4_687 and Pf1N1B4_688 are separated by 0 nucleotides
Pf1N1B4_685 - Nitrogen regulation protein NR(I), at 783,265 to 784,599
_685
Pf1N1B4_686 - Dicarboxylate MFS transporter, at 784,892 to 786,211
_686
Pf1N1B4_687 - Nitrilotriacetate monooxygenase component B (EC 1.14.13.-), at 786,453 to 787,082
_687
Pf1N1B4_688 - FIG00955483: hypothetical protein, at 787,083 to 787,313
_688
Position (kb) 784
785
786
787 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 783.965 kb on - strand, within Pf1N1B4_685 at 784.488 kb on + strand at 784.520 kb on + strand at 784.521 kb on - strand at 784.521 kb on - strand at 784.521 kb on - strand at 784.521 kb on - strand at 784.521 kb on - strand at 784.521 kb on - strand at 784.521 kb on - strand at 784.536 kb on + strand at 784.537 kb on - strand at 784.742 kb on + strand at 784.742 kb on + strand at 784.743 kb on - strand at 784.743 kb on - strand at 784.831 kb on - strand at 784.831 kb on - strand at 784.831 kb on - strand at 784.833 kb on - strand at 784.875 kb on + strand at 784.876 kb on - strand at 784.876 kb on - strand at 784.897 kb on + strand at 785.010 kb on - strand at 785.018 kb on + strand at 785.018 kb on + strand at 785.018 kb on + strand at 785.019 kb on - strand at 785.019 kb on - strand at 785.042 kb on + strand, within Pf1N1B4_686 at 785.180 kb on - strand, within Pf1N1B4_686 at 785.204 kb on + strand, within Pf1N1B4_686 at 785.205 kb on - strand, within Pf1N1B4_686 at 785.205 kb on - strand, within Pf1N1B4_686 at 785.249 kb on + strand, within Pf1N1B4_686 at 785.249 kb on + strand, within Pf1N1B4_686 at 785.345 kb on + strand, within Pf1N1B4_686 at 785.345 kb on + strand, within Pf1N1B4_686 at 785.346 kb on - strand at 785.346 kb on - strand, within Pf1N1B4_686 at 785.346 kb on - strand at 785.384 kb on + strand, within Pf1N1B4_686 at 785.385 kb on - strand, within Pf1N1B4_686 at 785.385 kb on - strand, within Pf1N1B4_686 at 785.480 kb on + strand, within Pf1N1B4_686 at 785.480 kb on + strand, within Pf1N1B4_686 at 785.681 kb on + strand, within Pf1N1B4_686 at 785.682 kb on - strand, within Pf1N1B4_686 at 785.682 kb on - strand, within Pf1N1B4_686 at 785.687 kb on + strand, within Pf1N1B4_686 at 785.687 kb on + strand, within Pf1N1B4_686 at 785.688 kb on - strand, within Pf1N1B4_686 at 785.696 kb on + strand, within Pf1N1B4_686 at 785.696 kb on + strand, within Pf1N1B4_686 at 785.696 kb on + strand, within Pf1N1B4_686 at 785.696 kb on + strand, within Pf1N1B4_686 at 785.696 kb on + strand, within Pf1N1B4_686 at 785.696 kb on + strand, within Pf1N1B4_686 at 785.696 kb on + strand at 785.696 kb on + strand, within Pf1N1B4_686 at 785.696 kb on + strand, within Pf1N1B4_686 at 785.696 kb on + strand, within Pf1N1B4_686 at 785.697 kb on - strand, within Pf1N1B4_686 at 785.697 kb on - strand, within Pf1N1B4_686 at 785.708 kb on + strand at 785.708 kb on + strand, within Pf1N1B4_686 at 785.709 kb on - strand, within Pf1N1B4_686 at 785.962 kb on + strand, within Pf1N1B4_686 at 785.962 kb on + strand at 785.963 kb on - strand, within Pf1N1B4_686 at 786.074 kb on + strand, within Pf1N1B4_686 at 786.074 kb on + strand, within Pf1N1B4_686 at 786.074 kb on + strand, within Pf1N1B4_686 at 786.074 kb on + strand, within Pf1N1B4_686 at 786.074 kb on + strand, within Pf1N1B4_686 at 786.074 kb on + strand, within Pf1N1B4_686 at 786.074 kb on + strand, within Pf1N1B4_686 at 786.075 kb on - strand, within Pf1N1B4_686 at 786.116 kb on + strand at 786.119 kb on + strand at 786.119 kb on + strand at 786.119 kb on + strand at 786.119 kb on + strand at 786.120 kb on - strand at 786.120 kb on - strand at 786.125 kb on + strand at 786.125 kb on + strand at 786.126 kb on - strand at 786.126 kb on - strand at 786.126 kb on - strand at 786.161 kb on + strand at 786.161 kb on + strand at 786.161 kb on + strand at 786.161 kb on + strand at 786.161 kb on + strand at 786.161 kb on + strand at 786.161 kb on + strand at 786.161 kb on + strand at 786.161 kb on + strand at 786.162 kb on - strand at 786.227 kb on - strand at 786.264 kb on + strand at 786.265 kb on - strand at 786.265 kb on - strand at 786.265 kb on - strand at 786.317 kb on - strand at 786.341 kb on + strand at 786.342 kb on - strand at 786.396 kb on + strand at 786.397 kb on - strand at 786.452 kb on + strand at 786.452 kb on + strand at 786.453 kb on - strand at 786.453 kb on - strand at 786.474 kb on + strand at 786.474 kb on + strand at 786.474 kb on + strand at 786.475 kb on - strand at 786.475 kb on - strand at 786.593 kb on + strand at 786.593 kb on + strand, within Pf1N1B4_687 at 786.593 kb on + strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.594 kb on - strand, within Pf1N1B4_687 at 786.615 kb on + strand, within Pf1N1B4_687 at 786.616 kb on - strand, within Pf1N1B4_687 at 786.616 kb on - strand, within Pf1N1B4_687 at 786.616 kb on - strand, within Pf1N1B4_687 at 786.972 kb on + strand, within Pf1N1B4_687 at 786.972 kb on + strand, within Pf1N1B4_687 at 786.973 kb on - strand, within Pf1N1B4_687 at 787.080 kb on + strand at 787.080 kb on + strand at 787.080 kb on + strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.081 kb on - strand at 787.084 kb on + strand at 787.084 kb on + strand at 787.085 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 783,965 - Pf1N1B4_685 0.52 -1.8 784,488 + -1.6 784,520 + -2.2 784,521 - -1.7 784,521 - -1.6 784,521 - -1.3 784,521 - -2.2 784,521 - -3.1 784,521 - +1.2 784,521 - -1.8 784,536 + -1.9 784,537 - -1.3 784,742 + -1.9 784,742 + -0.3 784,743 - -1.8 784,743 - -1.1 784,831 - -3.7 784,831 - -1.3 784,831 - -3.2 784,833 - -1.5 784,875 + -2.8 784,876 - -2.8 784,876 - -1.1 784,897 + -1.1 785,010 - +0.2 785,018 + -2.1 785,018 + -4.5 785,018 + +1.2 785,019 - -3.6 785,019 - -0.8 785,042 + Pf1N1B4_686 0.11 -2.2 785,180 - Pf1N1B4_686 0.22 -1.9 785,204 + Pf1N1B4_686 0.24 -0.3 785,205 - Pf1N1B4_686 0.24 -3.3 785,205 - Pf1N1B4_686 0.24 -1.1 785,249 + Pf1N1B4_686 0.27 -1.1 785,249 + Pf1N1B4_686 0.27 -1.3 785,345 + Pf1N1B4_686 0.34 -0.6 785,345 + Pf1N1B4_686 0.34 -1.3 785,346 - +0.2 785,346 - Pf1N1B4_686 0.34 -1.8 785,346 - +1.2 785,384 + Pf1N1B4_686 0.37 -2.0 785,385 - Pf1N1B4_686 0.37 -1.8 785,385 - Pf1N1B4_686 0.37 -1.8 785,480 + Pf1N1B4_686 0.45 -1.3 785,480 + Pf1N1B4_686 0.45 -2.8 785,681 + Pf1N1B4_686 0.60 -1.9 785,682 - Pf1N1B4_686 0.60 -2.5 785,682 - Pf1N1B4_686 0.60 -2.6 785,687 + Pf1N1B4_686 0.60 -1.3 785,687 + Pf1N1B4_686 0.60 -1.3 785,688 - Pf1N1B4_686 0.60 -0.5 785,696 + Pf1N1B4_686 0.61 -3.6 785,696 + Pf1N1B4_686 0.61 -3.3 785,696 + Pf1N1B4_686 0.61 -0.8 785,696 + Pf1N1B4_686 0.61 -1.1 785,696 + Pf1N1B4_686 0.61 -1.1 785,696 + Pf1N1B4_686 0.61 -4.2 785,696 + -1.3 785,696 + Pf1N1B4_686 0.61 +0.2 785,696 + Pf1N1B4_686 0.61 +0.4 785,696 + Pf1N1B4_686 0.61 -2.3 785,697 - Pf1N1B4_686 0.61 -1.6 785,697 - Pf1N1B4_686 0.61 -1.3 785,708 + -0.8 785,708 + Pf1N1B4_686 0.62 -2.8 785,709 - Pf1N1B4_686 0.62 -0.3 785,962 + Pf1N1B4_686 0.81 -3.5 785,962 + +0.2 785,963 - Pf1N1B4_686 0.81 -2.2 786,074 + Pf1N1B4_686 0.90 -1.1 786,074 + Pf1N1B4_686 0.90 -0.8 786,074 + Pf1N1B4_686 0.90 -1.3 786,074 + Pf1N1B4_686 0.90 -2.8 786,074 + Pf1N1B4_686 0.90 -2.9 786,074 + Pf1N1B4_686 0.90 -0.3 786,074 + Pf1N1B4_686 0.90 -2.5 786,075 - Pf1N1B4_686 0.90 -1.3 786,116 + -2.9 786,119 + -1.6 786,119 + +1.2 786,119 + -1.9 786,119 + -2.8 786,120 - +0.2 786,120 - -1.1 786,125 + -0.3 786,125 + +1.2 786,126 - +0.2 786,126 - -2.3 786,126 - -0.8 786,161 + -0.3 786,161 + -4.2 786,161 + -1.9 786,161 + -1.3 786,161 + -0.9 786,161 + -1.1 786,161 + -1.1 786,161 + +0.2 786,161 + -2.7 786,162 - -1.3 786,227 - -1.6 786,264 + +0.7 786,265 - +0.6 786,265 - -0.9 786,265 - -0.4 786,317 - -1.3 786,341 + +0.9 786,342 - -1.0 786,396 + +3.6 786,397 - +0.2 786,452 + +0.5 786,452 + -0.1 786,453 - +0.5 786,453 - -1.3 786,474 + +0.1 786,474 + +0.4 786,474 + -0.3 786,475 - -0.3 786,475 - -2.7 786,593 + +1.2 786,593 + Pf1N1B4_687 0.22 -0.5 786,593 + Pf1N1B4_687 0.22 +0.5 786,594 - Pf1N1B4_687 0.22 +1.2 786,594 - Pf1N1B4_687 0.22 +0.0 786,594 - Pf1N1B4_687 0.22 -0.1 786,594 - Pf1N1B4_687 0.22 -0.9 786,594 - Pf1N1B4_687 0.22 -0.3 786,594 - Pf1N1B4_687 0.22 -1.3 786,594 - Pf1N1B4_687 0.22 +0.2 786,594 - Pf1N1B4_687 0.22 -0.9 786,615 + Pf1N1B4_687 0.26 +0.0 786,616 - Pf1N1B4_687 0.26 -0.9 786,616 - Pf1N1B4_687 0.26 -1.3 786,616 - Pf1N1B4_687 0.26 +0.9 786,972 + Pf1N1B4_687 0.82 +0.8 786,972 + Pf1N1B4_687 0.82 -0.3 786,973 - Pf1N1B4_687 0.83 -0.1 787,080 + -1.6 787,080 + -0.9 787,080 + +0.3 787,081 - -0.2 787,081 - +0.2 787,081 - +0.7 787,081 - +2.7 787,081 - -0.5 787,081 - -1.9 787,081 - +0.2 787,084 + -0.9 787,084 + -0.2 787,085 - +0.2
Or see this region's nucleotide sequence