Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5060 and Pf1N1B4_5061 overlap by 4 nucleotides Pf1N1B4_5061 and Pf1N1B4_5062 are separated by 259 nucleotides Pf1N1B4_5062 and Pf1N1B4_5063 are separated by 36 nucleotides Pf1N1B4_5063 and Pf1N1B4_5064 are separated by 96 nucleotides
Pf1N1B4_5060 - Ferrichrome-iron receptor, at 5,604,935 to 5,607,097
_5060
Pf1N1B4_5061 - Valyl-tRNA synthetase, at 5,607,094 to 5,607,402
_5061
Pf1N1B4_5062 - Homospermidine synthase (EC 2.5.1.44), at 5,607,662 to 5,609,086
_5062
Pf1N1B4_5063 - Phosphoribosyl transferase domain protein, at 5,609,123 to 5,609,812
_5063
Pf1N1B4_5064 - Universal stress protein family 5, at 5,609,909 to 5,610,847
_5064
Position (kb) 5607
5608
5609
5610 Strain fitness (log2 ratio) -2
-1
0
1 at 5606.753 kb on + strand, within Pf1N1B4_5060 at 5606.753 kb on + strand, within Pf1N1B4_5060 at 5606.754 kb on - strand, within Pf1N1B4_5060 at 5606.909 kb on + strand at 5606.937 kb on - strand at 5607.026 kb on + strand at 5607.026 kb on + strand at 5607.026 kb on + strand at 5607.027 kb on - strand at 5607.027 kb on - strand at 5607.027 kb on - strand at 5607.134 kb on + strand, within Pf1N1B4_5061 at 5607.135 kb on - strand, within Pf1N1B4_5061 at 5607.147 kb on + strand, within Pf1N1B4_5061 at 5607.179 kb on + strand, within Pf1N1B4_5061 at 5607.179 kb on + strand, within Pf1N1B4_5061 at 5607.179 kb on + strand, within Pf1N1B4_5061 at 5607.180 kb on - strand, within Pf1N1B4_5061 at 5607.180 kb on - strand, within Pf1N1B4_5061 at 5607.876 kb on - strand, within Pf1N1B4_5062 at 5608.024 kb on + strand at 5608.025 kb on - strand, within Pf1N1B4_5062 at 5608.062 kb on - strand, within Pf1N1B4_5062 at 5608.062 kb on - strand, within Pf1N1B4_5062 at 5608.062 kb on - strand, within Pf1N1B4_5062 at 5608.431 kb on - strand, within Pf1N1B4_5062 at 5608.434 kb on - strand, within Pf1N1B4_5062 at 5608.463 kb on + strand, within Pf1N1B4_5062 at 5608.463 kb on + strand at 5608.464 kb on - strand at 5608.464 kb on - strand, within Pf1N1B4_5062 at 5608.572 kb on - strand, within Pf1N1B4_5062 at 5608.779 kb on - strand at 5608.785 kb on - strand, within Pf1N1B4_5062 at 5608.978 kb on - strand at 5608.978 kb on - strand at 5608.989 kb on - strand at 5608.989 kb on - strand at 5609.233 kb on - strand, within Pf1N1B4_5063 at 5609.474 kb on + strand, within Pf1N1B4_5063 at 5609.474 kb on + strand, within Pf1N1B4_5063 at 5609.475 kb on - strand, within Pf1N1B4_5063 at 5609.688 kb on + strand, within Pf1N1B4_5063 at 5609.689 kb on - strand, within Pf1N1B4_5063 at 5609.689 kb on - strand at 5609.744 kb on - strand at 5609.821 kb on + strand at 5609.948 kb on - strand at 5609.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 5,606,753 + Pf1N1B4_5060 0.84 -0.6 5,606,753 + Pf1N1B4_5060 0.84 -0.5 5,606,754 - Pf1N1B4_5060 0.84 -0.0 5,606,909 + +0.6 5,606,937 - -0.0 5,607,026 + -1.4 5,607,026 + -2.2 5,607,026 + +0.2 5,607,027 - +0.3 5,607,027 - -2.5 5,607,027 - -1.2 5,607,134 + Pf1N1B4_5061 0.13 +0.5 5,607,135 - Pf1N1B4_5061 0.13 -0.5 5,607,147 + Pf1N1B4_5061 0.17 -0.4 5,607,179 + Pf1N1B4_5061 0.28 -2.5 5,607,179 + Pf1N1B4_5061 0.28 +0.6 5,607,179 + Pf1N1B4_5061 0.28 -1.1 5,607,180 - Pf1N1B4_5061 0.28 +0.2 5,607,180 - Pf1N1B4_5061 0.28 +0.8 5,607,876 - Pf1N1B4_5062 0.15 +1.1 5,608,024 + -0.4 5,608,025 - Pf1N1B4_5062 0.25 -1.1 5,608,062 - Pf1N1B4_5062 0.28 -0.1 5,608,062 - Pf1N1B4_5062 0.28 +0.4 5,608,062 - Pf1N1B4_5062 0.28 +1.6 5,608,431 - Pf1N1B4_5062 0.54 -1.3 5,608,434 - Pf1N1B4_5062 0.54 -1.7 5,608,463 + Pf1N1B4_5062 0.56 +0.6 5,608,463 + +1.2 5,608,464 - -1.7 5,608,464 - Pf1N1B4_5062 0.56 +1.2 5,608,572 - Pf1N1B4_5062 0.64 +0.1 5,608,779 - -0.7 5,608,785 - Pf1N1B4_5062 0.79 -0.4 5,608,978 - -0.4 5,608,978 - -0.4 5,608,989 - -1.2 5,608,989 - +0.4 5,609,233 - Pf1N1B4_5063 0.16 -0.7 5,609,474 + Pf1N1B4_5063 0.51 -0.3 5,609,474 + Pf1N1B4_5063 0.51 +0.6 5,609,475 - Pf1N1B4_5063 0.51 -1.2 5,609,688 + Pf1N1B4_5063 0.82 -1.7 5,609,689 - Pf1N1B4_5063 0.82 -0.9 5,609,689 - -0.4 5,609,744 - -0.8 5,609,821 + -0.1 5,609,948 - +1.2 5,609,948 - -0.6
Or see this region's nucleotide sequence