Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3860 and Pf1N1B4_3861 are separated by 123 nucleotides Pf1N1B4_3861 and Pf1N1B4_3862 are separated by 144 nucleotides
Pf1N1B4_3860 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 4,288,162 to 4,289,871
_3860
Pf1N1B4_3861 - FIG00953368: hypothetical protein, at 4,289,995 to 4,290,738
_3861
Pf1N1B4_3862 - DNA polymerase III subunits gamma and tau (EC 2.7.7.7), at 4,290,883 to 4,292,970
_3862
Position (kb)
4289
4290
4291 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4289.025 kb on - strand, within Pf1N1B4_3860 at 4289.334 kb on + strand, within Pf1N1B4_3860 at 4289.335 kb on - strand, within Pf1N1B4_3860 at 4289.394 kb on - strand, within Pf1N1B4_3860 at 4289.394 kb on - strand, within Pf1N1B4_3860 at 4289.560 kb on + strand, within Pf1N1B4_3860 at 4289.560 kb on + strand, within Pf1N1B4_3860 at 4289.573 kb on + strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.574 kb on - strand, within Pf1N1B4_3860 at 4289.577 kb on + strand, within Pf1N1B4_3860 at 4289.598 kb on + strand, within Pf1N1B4_3860 at 4289.598 kb on + strand, within Pf1N1B4_3860 at 4289.599 kb on - strand, within Pf1N1B4_3860 at 4289.599 kb on - strand, within Pf1N1B4_3860 at 4289.764 kb on + strand at 4289.764 kb on + strand at 4289.909 kb on + strand at 4289.920 kb on + strand at 4290.018 kb on - strand at 4290.111 kb on + strand, within Pf1N1B4_3861 at 4290.111 kb on + strand, within Pf1N1B4_3861 at 4290.112 kb on - strand, within Pf1N1B4_3861 at 4290.112 kb on - strand, within Pf1N1B4_3861 at 4290.112 kb on - strand, within Pf1N1B4_3861 at 4290.112 kb on - strand at 4290.113 kb on + strand, within Pf1N1B4_3861 at 4290.114 kb on - strand at 4290.114 kb on - strand at 4290.114 kb on - strand, within Pf1N1B4_3861 at 4290.114 kb on - strand, within Pf1N1B4_3861 at 4290.114 kb on - strand, within Pf1N1B4_3861 at 4290.145 kb on + strand, within Pf1N1B4_3861 at 4290.218 kb on + strand, within Pf1N1B4_3861 at 4290.218 kb on + strand, within Pf1N1B4_3861 at 4290.321 kb on - strand, within Pf1N1B4_3861 at 4290.324 kb on + strand, within Pf1N1B4_3861 at 4290.324 kb on + strand at 4290.325 kb on - strand at 4290.325 kb on - strand at 4290.325 kb on - strand, within Pf1N1B4_3861 at 4290.325 kb on - strand, within Pf1N1B4_3861 at 4290.327 kb on - strand at 4290.327 kb on - strand at 4290.327 kb on - strand, within Pf1N1B4_3861 at 4290.443 kb on + strand, within Pf1N1B4_3861 at 4290.443 kb on + strand, within Pf1N1B4_3861 at 4290.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 4,289,025 - Pf1N1B4_3860 0.50 -0.2 4,289,334 + Pf1N1B4_3860 0.69 +0.6 4,289,335 - Pf1N1B4_3860 0.69 -0.5 4,289,394 - Pf1N1B4_3860 0.72 -1.3 4,289,394 - Pf1N1B4_3860 0.72 -1.6 4,289,560 + Pf1N1B4_3860 0.82 -0.0 4,289,560 + Pf1N1B4_3860 0.82 +1.2 4,289,573 + Pf1N1B4_3860 0.83 +0.6 4,289,574 - Pf1N1B4_3860 0.83 -0.0 4,289,574 - Pf1N1B4_3860 0.83 +0.3 4,289,574 - Pf1N1B4_3860 0.83 -1.8 4,289,574 - Pf1N1B4_3860 0.83 -1.0 4,289,574 - Pf1N1B4_3860 0.83 -0.3 4,289,577 + Pf1N1B4_3860 0.83 -0.4 4,289,598 + Pf1N1B4_3860 0.84 +0.7 4,289,598 + Pf1N1B4_3860 0.84 +1.2 4,289,599 - Pf1N1B4_3860 0.84 +1.0 4,289,599 - Pf1N1B4_3860 0.84 -0.6 4,289,764 + +1.6 4,289,764 + +0.6 4,289,909 + -1.0 4,289,920 + +0.2 4,290,018 - -0.6 4,290,111 + Pf1N1B4_3861 0.16 -1.2 4,290,111 + Pf1N1B4_3861 0.16 -0.6 4,290,112 - Pf1N1B4_3861 0.16 -1.6 4,290,112 - Pf1N1B4_3861 0.16 -1.7 4,290,112 - Pf1N1B4_3861 0.16 -1.6 4,290,112 - +1.0 4,290,113 + Pf1N1B4_3861 0.16 -0.6 4,290,114 - -0.0 4,290,114 - -0.0 4,290,114 - Pf1N1B4_3861 0.16 -0.3 4,290,114 - Pf1N1B4_3861 0.16 -3.9 4,290,114 - Pf1N1B4_3861 0.16 -2.6 4,290,145 + Pf1N1B4_3861 0.20 +0.8 4,290,218 + Pf1N1B4_3861 0.30 -0.3 4,290,218 + Pf1N1B4_3861 0.30 +0.7 4,290,321 - Pf1N1B4_3861 0.44 -0.6 4,290,324 + Pf1N1B4_3861 0.44 +0.1 4,290,324 + -0.6 4,290,325 - -1.3 4,290,325 - -0.0 4,290,325 - Pf1N1B4_3861 0.44 -0.0 4,290,325 - Pf1N1B4_3861 0.44 -0.0 4,290,327 - +1.7 4,290,327 - +0.4 4,290,327 - Pf1N1B4_3861 0.45 -0.8 4,290,443 + Pf1N1B4_3861 0.60 -0.0 4,290,443 + Pf1N1B4_3861 0.60 +1.0 4,290,771 + +1.0
Or see this region's nucleotide sequence