Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5022 and Pf6N2E2_5023 are separated by 28 nucleotides Pf6N2E2_5023 and Pf6N2E2_5024 are separated by 17 nucleotides
Pf6N2E2_5022 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 5,787,438 to 5,788,721
_5022
Pf6N2E2_5023 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 5,788,750 to 5,789,376
_5023
Pf6N2E2_5024 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 5,789,394 to 5,790,122
_5024
Position (kb)
5788
5789
5790 Strain fitness (log2 ratio)
-2
-1
0
1 at 5788.752 kb on - strand at 5788.752 kb on - strand at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.010 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.309 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.313 kb on - strand, within Pf6N2E2_5023 at 5789.358 kb on - strand at 5789.369 kb on - strand at 5789.480 kb on - strand, within Pf6N2E2_5024 at 5789.480 kb on - strand, within Pf6N2E2_5024 at 5789.573 kb on - strand, within Pf6N2E2_5024 at 5789.598 kb on - strand, within Pf6N2E2_5024 at 5790.111 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.182 kb on - strand at 5790.186 kb on - strand at 5790.186 kb on - strand at 5790.186 kb on - strand at 5790.186 kb on - strand at 5790.205 kb on - strand at 5790.237 kb on + strand at 5790.237 kb on + strand at 5790.241 kb on - strand at 5790.241 kb on - strand at 5790.241 kb on - strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.253 kb on + strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.254 kb on - strand at 5790.255 kb on + strand at 5790.255 kb on + strand at 5790.255 kb on + strand at 5790.256 kb on - strand at 5790.256 kb on - strand at 5790.256 kb on - strand at 5790.266 kb on + strand at 5790.266 kb on + strand at 5790.267 kb on - strand at 5790.267 kb on - strand at 5790.283 kb on + strand at 5790.284 kb on - strand at 5790.284 kb on - strand at 5790.346 kb on + strand at 5790.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,788,752 - +0.2 5,788,752 - -0.2 5,789,010 - Pf6N2E2_5023 0.41 -0.5 5,789,010 - Pf6N2E2_5023 0.41 -0.9 5,789,010 - Pf6N2E2_5023 0.41 -0.9 5,789,309 - Pf6N2E2_5023 0.89 -1.0 5,789,309 - Pf6N2E2_5023 0.89 +0.3 5,789,309 - Pf6N2E2_5023 0.89 -0.2 5,789,309 - Pf6N2E2_5023 0.89 -0.4 5,789,313 - Pf6N2E2_5023 0.90 -0.3 5,789,313 - Pf6N2E2_5023 0.90 -1.2 5,789,313 - Pf6N2E2_5023 0.90 -0.8 5,789,313 - Pf6N2E2_5023 0.90 -1.1 5,789,358 - -0.1 5,789,369 - +0.3 5,789,480 - Pf6N2E2_5024 0.12 -0.1 5,789,480 - Pf6N2E2_5024 0.12 -0.2 5,789,573 - Pf6N2E2_5024 0.25 -0.9 5,789,598 - Pf6N2E2_5024 0.28 -0.1 5,790,111 - -0.3 5,790,182 - -0.0 5,790,182 - -0.2 5,790,182 - +0.2 5,790,182 - +0.8 5,790,182 - +0.8 5,790,182 - -0.5 5,790,186 - +0.7 5,790,186 - +0.8 5,790,186 - +1.0 5,790,186 - +0.3 5,790,205 - -0.0 5,790,237 + +1.7 5,790,237 + +0.1 5,790,241 - +1.1 5,790,241 - +0.6 5,790,241 - -0.3 5,790,253 + -0.1 5,790,253 + -0.4 5,790,253 + +0.4 5,790,253 + +0.2 5,790,253 + -0.6 5,790,253 + -0.4 5,790,254 - +0.6 5,790,254 - -1.0 5,790,254 - +0.6 5,790,254 - -2.3 5,790,254 - +0.2 5,790,254 - +0.1 5,790,254 - -0.6 5,790,254 - +0.2 5,790,254 - +0.1 5,790,255 + -0.2 5,790,255 + +0.5 5,790,255 + -0.7 5,790,256 - +0.2 5,790,256 - +0.4 5,790,256 - +0.9 5,790,266 + +0.4 5,790,266 + +1.6 5,790,267 - +0.4 5,790,267 - +0.4 5,790,283 + +0.7 5,790,284 - +1.1 5,790,284 - -1.5 5,790,346 + -1.7 5,790,375 - -0.4
Or see this region's nucleotide sequence