Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_478

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_477 and Pf6N2E2_478 are separated by 283 nucleotidesPf6N2E2_478 and Pf6N2E2_479 are separated by 3 nucleotides Pf6N2E2_477 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 542,197 to 543,216 _477 Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data), at 543,500 to 544,885 _478 Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data), at 544,889 to 546,160 _479 Position (kb) 543 544 545Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 542.652 kb on - strand, within Pf6N2E2_477at 542.747 kb on + strand, within Pf6N2E2_477at 542.843 kb on + strand, within Pf6N2E2_477at 543.173 kb on + strandat 543.174 kb on - strandat 543.174 kb on - strandat 543.226 kb on + strandat 543.226 kb on + strandat 543.227 kb on - strandat 543.227 kb on - strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.288 kb on + strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.289 kb on - strandat 543.501 kb on + strandat 544.195 kb on - strand, within Pf6N2E2_478at 544.195 kb on - strand, within Pf6N2E2_478at 544.341 kb on + strand, within Pf6N2E2_478at 544.342 kb on - strand, within Pf6N2E2_478at 544.830 kb on + strandat 544.891 kb on - strandat 545.075 kb on + strand, within Pf6N2E2_479at 545.497 kb on - strand, within Pf6N2E2_479at 545.599 kb on - strand, within Pf6N2E2_479at 545.599 kb on - strand, within Pf6N2E2_479at 545.643 kb on + strand, within Pf6N2E2_479at 545.644 kb on - strand, within Pf6N2E2_479at 545.644 kb on - strand, within Pf6N2E2_479

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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542,652 - Pf6N2E2_477 0.45 -1.7
542,747 + Pf6N2E2_477 0.54 -3.4
542,843 + Pf6N2E2_477 0.63 -4.6
543,173 + -3.9
543,174 - -3.1
543,174 - -3.7
543,226 + -3.9
543,226 + -2.1
543,227 - -2.4
543,227 - +0.8
543,288 + -3.7
543,288 + -3.8
543,288 + -4.6
543,288 + -3.5
543,288 + -2.8
543,288 + -3.8
543,289 - -0.2
543,289 - +0.1
543,289 - +0.3
543,289 - +0.4
543,289 - +0.3
543,289 - -0.8
543,289 - -0.3
543,289 - +0.3
543,289 - -0.1
543,501 + -0.9
544,195 - Pf6N2E2_478 0.50 -1.1
544,195 - Pf6N2E2_478 0.50 -3.0
544,341 + Pf6N2E2_478 0.61 -3.3
544,342 - Pf6N2E2_478 0.61 -4.4
544,830 + -2.4
544,891 - -4.0
545,075 + Pf6N2E2_479 0.15 -3.7
545,497 - Pf6N2E2_479 0.48 -2.6
545,599 - Pf6N2E2_479 0.56 -3.2
545,599 - Pf6N2E2_479 0.56 -4.4
545,643 + Pf6N2E2_479 0.59 -4.1
545,644 - Pf6N2E2_479 0.59 -3.1
545,644 - Pf6N2E2_479 0.59 -4.4

Or see this region's nucleotide sequence