Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4549 and Pf6N2E2_4550 are separated by 14 nucleotides Pf6N2E2_4550 and Pf6N2E2_4551 are separated by 52 nucleotides Pf6N2E2_4551 and Pf6N2E2_4552 are separated by 293 nucleotides Pf6N2E2_4552 and Pf6N2E2_4553 are separated by 93 nucleotides
Pf6N2E2_4549 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 5,279,744 to 5,280,631
_4549
Pf6N2E2_4550 - hypothetical protein, at 5,280,646 to 5,281,020
_4550
Pf6N2E2_4551 - hypothetical protein, at 5,281,073 to 5,281,750
_4551
Pf6N2E2_4552 - hypothetical protein, at 5,282,044 to 5,282,253
_4552
Pf6N2E2_4553 - Nitrogen regulation protein NtrC, at 5,282,347 to 5,283,270
_4553
Position (kb)
5281
5282 Strain fitness (log2 ratio)
-1
0
1 at 5280.172 kb on + strand, within Pf6N2E2_4549 at 5280.172 kb on + strand, within Pf6N2E2_4549 at 5280.173 kb on - strand, within Pf6N2E2_4549 at 5280.181 kb on + strand, within Pf6N2E2_4549 at 5280.272 kb on + strand, within Pf6N2E2_4549 at 5280.312 kb on + strand, within Pf6N2E2_4549 at 5280.312 kb on + strand, within Pf6N2E2_4549 at 5280.312 kb on + strand, within Pf6N2E2_4549 at 5280.312 kb on + strand, within Pf6N2E2_4549 at 5280.312 kb on + strand, within Pf6N2E2_4549 at 5280.312 kb on + strand, within Pf6N2E2_4549 at 5280.312 kb on + strand, within Pf6N2E2_4549 at 5280.312 kb on + strand, within Pf6N2E2_4549 at 5280.313 kb on - strand, within Pf6N2E2_4549 at 5280.313 kb on - strand, within Pf6N2E2_4549 at 5280.313 kb on - strand, within Pf6N2E2_4549 at 5280.313 kb on - strand, within Pf6N2E2_4549 at 5280.319 kb on - strand, within Pf6N2E2_4549 at 5280.648 kb on - strand at 5281.012 kb on + strand at 5281.012 kb on + strand at 5281.012 kb on + strand at 5281.012 kb on + strand at 5281.012 kb on + strand at 5281.012 kb on + strand at 5281.013 kb on - strand at 5281.013 kb on - strand at 5281.013 kb on - strand at 5281.013 kb on - strand at 5281.013 kb on - strand at 5281.668 kb on + strand, within Pf6N2E2_4551 at 5281.668 kb on + strand, within Pf6N2E2_4551 at 5281.669 kb on - strand, within Pf6N2E2_4551 at 5281.669 kb on - strand, within Pf6N2E2_4551 at 5281.739 kb on + strand at 5282.053 kb on + strand at 5282.053 kb on + strand at 5282.053 kb on + strand at 5282.053 kb on + strand at 5282.253 kb on + strand at 5282.254 kb on - strand at 5282.254 kb on - strand at 5282.259 kb on + strand at 5282.260 kb on - strand at 5282.260 kb on - strand at 5282.307 kb on + strand at 5282.359 kb on + strand at 5282.359 kb on + strand at 5282.384 kb on + strand at 5282.384 kb on + strand at 5282.385 kb on - strand at 5282.385 kb on - strand at 5282.385 kb on - strand at 5282.560 kb on + strand, within Pf6N2E2_4553 at 5282.560 kb on + strand, within Pf6N2E2_4553 at 5282.560 kb on + strand, within Pf6N2E2_4553 at 5282.561 kb on - strand, within Pf6N2E2_4553 at 5282.561 kb on - strand, within Pf6N2E2_4553 at 5282.654 kb on + strand, within Pf6N2E2_4553
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,280,172 + Pf6N2E2_4549 0.48 +0.3 5,280,172 + Pf6N2E2_4549 0.48 -0.2 5,280,173 - Pf6N2E2_4549 0.48 +0.7 5,280,181 + Pf6N2E2_4549 0.49 +0.4 5,280,272 + Pf6N2E2_4549 0.59 -0.2 5,280,312 + Pf6N2E2_4549 0.64 +0.7 5,280,312 + Pf6N2E2_4549 0.64 +0.4 5,280,312 + Pf6N2E2_4549 0.64 -0.9 5,280,312 + Pf6N2E2_4549 0.64 +0.6 5,280,312 + Pf6N2E2_4549 0.64 -0.2 5,280,312 + Pf6N2E2_4549 0.64 -0.4 5,280,312 + Pf6N2E2_4549 0.64 +0.9 5,280,312 + Pf6N2E2_4549 0.64 +0.1 5,280,313 - Pf6N2E2_4549 0.64 -0.1 5,280,313 - Pf6N2E2_4549 0.64 +0.0 5,280,313 - Pf6N2E2_4549 0.64 +0.4 5,280,313 - Pf6N2E2_4549 0.64 +0.2 5,280,319 - Pf6N2E2_4549 0.65 +0.7 5,280,648 - +0.1 5,281,012 + +0.2 5,281,012 + +0.1 5,281,012 + +0.0 5,281,012 + +0.7 5,281,012 + +0.7 5,281,012 + +0.2 5,281,013 - -0.4 5,281,013 - -1.2 5,281,013 - +0.0 5,281,013 - +1.2 5,281,013 - -0.1 5,281,668 + Pf6N2E2_4551 0.88 -0.0 5,281,668 + Pf6N2E2_4551 0.88 -0.1 5,281,669 - Pf6N2E2_4551 0.88 -1.2 5,281,669 - Pf6N2E2_4551 0.88 -0.8 5,281,739 + +0.2 5,282,053 + +1.1 5,282,053 + -0.2 5,282,053 + +0.4 5,282,053 + -0.8 5,282,253 + +0.5 5,282,254 - -0.5 5,282,254 - +1.1 5,282,259 + +0.6 5,282,260 - +0.3 5,282,260 - -0.1 5,282,307 + -0.1 5,282,359 + +0.2 5,282,359 + +0.2 5,282,384 + -0.4 5,282,384 + +0.6 5,282,385 - -0.3 5,282,385 - -0.2 5,282,385 - +0.3 5,282,560 + Pf6N2E2_4553 0.23 +0.7 5,282,560 + Pf6N2E2_4553 0.23 +0.2 5,282,560 + Pf6N2E2_4553 0.23 +1.2 5,282,561 - Pf6N2E2_4553 0.23 +0.8 5,282,561 - Pf6N2E2_4553 0.23 +1.0 5,282,654 + Pf6N2E2_4553 0.33 +0.5
Or see this region's nucleotide sequence