Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3616

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3615 and Pf6N2E2_3616 overlap by 1 nucleotidesPf6N2E2_3616 and Pf6N2E2_3617 overlap by 4 nucleotides Pf6N2E2_3615 - tRNA dimethylallyltransferase (EC 2.5.1.75), at 4,209,510 to 4,210,481 _3615 Pf6N2E2_3616 - DNA mismatch repair protein MutL, at 4,210,481 to 4,212,394 _3616 Pf6N2E2_3617 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 4,212,391 to 4,213,818 _3617 Position (kb) 4210 4211 4212 4213Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4209.491 kb on - strandat 4209.520 kb on - strandat 4209.539 kb on - strandat 4210.705 kb on - strand, within Pf6N2E2_3616at 4210.705 kb on - strand, within Pf6N2E2_3616at 4210.705 kb on - strand, within Pf6N2E2_3616at 4210.954 kb on - strand, within Pf6N2E2_3616at 4210.954 kb on - strand, within Pf6N2E2_3616at 4210.954 kb on - strand, within Pf6N2E2_3616at 4210.954 kb on - strand, within Pf6N2E2_3616at 4211.021 kb on - strand, within Pf6N2E2_3616at 4211.021 kb on - strand, within Pf6N2E2_3616at 4211.022 kb on + strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.023 kb on - strand, within Pf6N2E2_3616at 4211.182 kb on - strand, within Pf6N2E2_3616at 4211.182 kb on - strand, within Pf6N2E2_3616at 4211.182 kb on - strand, within Pf6N2E2_3616at 4211.182 kb on - strand, within Pf6N2E2_3616at 4211.182 kb on - strand, within Pf6N2E2_3616at 4211.188 kb on - strand, within Pf6N2E2_3616at 4211.188 kb on - strand, within Pf6N2E2_3616at 4211.227 kb on - strand, within Pf6N2E2_3616at 4211.404 kb on - strand, within Pf6N2E2_3616at 4211.442 kb on - strand, within Pf6N2E2_3616at 4211.442 kb on - strand, within Pf6N2E2_3616at 4211.442 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.463 kb on - strand, within Pf6N2E2_3616at 4211.601 kb on + strand, within Pf6N2E2_3616at 4211.602 kb on - strand, within Pf6N2E2_3616at 4211.602 kb on - strand, within Pf6N2E2_3616at 4211.608 kb on - strand, within Pf6N2E2_3616at 4212.038 kb on - strand, within Pf6N2E2_3616at 4212.596 kb on + strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617at 4212.597 kb on - strand, within Pf6N2E2_3617

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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4,209,491 - +2.6
4,209,520 - +2.9
4,209,539 - +1.6
4,210,705 - Pf6N2E2_3616 0.12 +0.2
4,210,705 - Pf6N2E2_3616 0.12 +0.3
4,210,705 - Pf6N2E2_3616 0.12 +0.9
4,210,954 - Pf6N2E2_3616 0.25 +0.6
4,210,954 - Pf6N2E2_3616 0.25 -0.1
4,210,954 - Pf6N2E2_3616 0.25 -1.0
4,210,954 - Pf6N2E2_3616 0.25 +0.1
4,211,021 - Pf6N2E2_3616 0.28 -0.1
4,211,021 - Pf6N2E2_3616 0.28 -2.2
4,211,022 + Pf6N2E2_3616 0.28 -0.5
4,211,023 - Pf6N2E2_3616 0.28 -1.6
4,211,023 - Pf6N2E2_3616 0.28 +0.8
4,211,023 - Pf6N2E2_3616 0.28 -0.7
4,211,023 - Pf6N2E2_3616 0.28 -0.3
4,211,023 - Pf6N2E2_3616 0.28 -0.6
4,211,023 - Pf6N2E2_3616 0.28 -0.7
4,211,023 - Pf6N2E2_3616 0.28 -0.4
4,211,023 - Pf6N2E2_3616 0.28 +0.0
4,211,023 - Pf6N2E2_3616 0.28 -1.0
4,211,023 - Pf6N2E2_3616 0.28 -0.8
4,211,182 - Pf6N2E2_3616 0.37 -1.1
4,211,182 - Pf6N2E2_3616 0.37 -0.3
4,211,182 - Pf6N2E2_3616 0.37 -0.5
4,211,182 - Pf6N2E2_3616 0.37 -2.5
4,211,182 - Pf6N2E2_3616 0.37 -0.6
4,211,188 - Pf6N2E2_3616 0.37 -0.4
4,211,188 - Pf6N2E2_3616 0.37 -0.7
4,211,227 - Pf6N2E2_3616 0.39 -1.0
4,211,404 - Pf6N2E2_3616 0.48 +0.0
4,211,442 - Pf6N2E2_3616 0.50 -0.9
4,211,442 - Pf6N2E2_3616 0.50 -0.4
4,211,442 - Pf6N2E2_3616 0.50 -0.2
4,211,463 - Pf6N2E2_3616 0.51 -1.9
4,211,463 - Pf6N2E2_3616 0.51 +0.0
4,211,463 - Pf6N2E2_3616 0.51 -1.4
4,211,463 - Pf6N2E2_3616 0.51 +0.1
4,211,463 - Pf6N2E2_3616 0.51 -2.1
4,211,601 + Pf6N2E2_3616 0.59 +0.9
4,211,602 - Pf6N2E2_3616 0.59 -0.5
4,211,602 - Pf6N2E2_3616 0.59 -0.5
4,211,608 - Pf6N2E2_3616 0.59 -0.6
4,212,038 - Pf6N2E2_3616 0.81 -1.9
4,212,596 + Pf6N2E2_3617 0.14 -2.8
4,212,597 - Pf6N2E2_3617 0.14 +1.4
4,212,597 - Pf6N2E2_3617 0.14 -0.4
4,212,597 - Pf6N2E2_3617 0.14 -0.7
4,212,597 - Pf6N2E2_3617 0.14 +0.4
4,212,597 - Pf6N2E2_3617 0.14 -0.7
4,212,597 - Pf6N2E2_3617 0.14 -0.4
4,212,597 - Pf6N2E2_3617 0.14 -0.9
4,212,597 - Pf6N2E2_3617 0.14 -0.2
4,212,597 - Pf6N2E2_3617 0.14 +0.6
4,212,597 - Pf6N2E2_3617 0.14 +0.0
4,212,597 - Pf6N2E2_3617 0.14 +0.1

Or see this region's nucleotide sequence