Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1137 and Pf6N2E2_1138 overlap by 4 nucleotides Pf6N2E2_1138 and Pf6N2E2_1139 are separated by 152 nucleotides
Pf6N2E2_1137 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 1,333,235 to 1,334,578
_1137
Pf6N2E2_1138 - Transcriptional regulator, AraC family, at 1,334,575 to 1,335,606
_1138
Pf6N2E2_1139 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,335,759 to 1,336,730
_1139
Position (kb)
1334
1335
1336 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1333.589 kb on + strand, within Pf6N2E2_1137 at 1333.590 kb on - strand, within Pf6N2E2_1137 at 1333.590 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.694 kb on + strand, within Pf6N2E2_1137 at 1333.890 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1334.039 kb on + strand, within Pf6N2E2_1137 at 1334.039 kb on + strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137 at 1334.141 kb on + strand, within Pf6N2E2_1137 at 1334.142 kb on - strand, within Pf6N2E2_1137 at 1334.142 kb on - strand, within Pf6N2E2_1137 at 1334.142 kb on - strand, within Pf6N2E2_1137 at 1334.142 kb on - strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.193 kb on - strand, within Pf6N2E2_1137 at 1334.265 kb on + strand, within Pf6N2E2_1137 at 1334.266 kb on - strand, within Pf6N2E2_1137 at 1334.266 kb on - strand, within Pf6N2E2_1137 at 1334.266 kb on - strand, within Pf6N2E2_1137 at 1334.288 kb on + strand, within Pf6N2E2_1137 at 1334.288 kb on + strand, within Pf6N2E2_1137 at 1334.288 kb on + strand, within Pf6N2E2_1137 at 1334.288 kb on + strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.448 kb on - strand at 1334.474 kb on + strand at 1334.607 kb on + strand at 1334.607 kb on + strand at 1334.607 kb on + strand at 1334.608 kb on - strand at 1334.608 kb on - strand at 1334.675 kb on + strand at 1334.676 kb on - strand at 1334.687 kb on + strand, within Pf6N2E2_1138 at 1334.687 kb on + strand, within Pf6N2E2_1138 at 1334.687 kb on + strand, within Pf6N2E2_1138 at 1334.743 kb on + strand, within Pf6N2E2_1138 at 1334.744 kb on - strand, within Pf6N2E2_1138 at 1335.004 kb on + strand, within Pf6N2E2_1138 at 1335.004 kb on + strand, within Pf6N2E2_1138 at 1335.005 kb on - strand, within Pf6N2E2_1138 at 1335.005 kb on - strand, within Pf6N2E2_1138 at 1335.013 kb on + strand, within Pf6N2E2_1138 at 1335.013 kb on + strand, within Pf6N2E2_1138 at 1335.014 kb on - strand, within Pf6N2E2_1138 at 1335.533 kb on - strand at 1335.533 kb on - strand at 1335.661 kb on + strand at 1335.674 kb on + strand at 1335.675 kb on - strand at 1335.727 kb on + strand at 1335.727 kb on + strand at 1335.727 kb on + strand at 1335.728 kb on - strand at 1335.728 kb on - strand at 1335.728 kb on - strand at 1335.728 kb on - strand at 1335.733 kb on - strand at 1335.758 kb on + strand at 1335.759 kb on - strand at 1335.865 kb on - strand, within Pf6N2E2_1139 at 1335.867 kb on + strand, within Pf6N2E2_1139 at 1335.867 kb on + strand, within Pf6N2E2_1139 at 1335.868 kb on - strand, within Pf6N2E2_1139 at 1335.868 kb on - strand, within Pf6N2E2_1139 at 1336.053 kb on + strand, within Pf6N2E2_1139 at 1336.054 kb on - strand, within Pf6N2E2_1139 at 1336.072 kb on + strand, within Pf6N2E2_1139 at 1336.072 kb on + strand, within Pf6N2E2_1139 at 1336.073 kb on - strand, within Pf6N2E2_1139 at 1336.174 kb on - strand, within Pf6N2E2_1139 at 1336.266 kb on + strand, within Pf6N2E2_1139 at 1336.305 kb on + strand, within Pf6N2E2_1139 at 1336.305 kb on + strand, within Pf6N2E2_1139 at 1336.306 kb on - strand, within Pf6N2E2_1139 at 1336.306 kb on - strand, within Pf6N2E2_1139 at 1336.416 kb on + strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.418 kb on + strand, within Pf6N2E2_1139 at 1336.497 kb on + strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,333,589 + Pf6N2E2_1137 0.26 +2.5 1,333,590 - Pf6N2E2_1137 0.26 -1.1 1,333,590 - Pf6N2E2_1137 0.26 -2.1 1,333,688 - Pf6N2E2_1137 0.34 -1.0 1,333,688 - Pf6N2E2_1137 0.34 -0.8 1,333,688 - Pf6N2E2_1137 0.34 -1.8 1,333,694 + Pf6N2E2_1137 0.34 +0.9 1,333,890 - Pf6N2E2_1137 0.49 -0.9 1,333,899 - Pf6N2E2_1137 0.49 -1.4 1,333,899 - Pf6N2E2_1137 0.49 -0.9 1,333,899 - Pf6N2E2_1137 0.49 -0.9 1,333,899 - Pf6N2E2_1137 0.49 +0.1 1,334,039 + Pf6N2E2_1137 0.60 +1.8 1,334,039 + Pf6N2E2_1137 0.60 -0.4 1,334,040 - Pf6N2E2_1137 0.60 -2.6 1,334,040 - Pf6N2E2_1137 0.60 +0.6 1,334,040 - Pf6N2E2_1137 0.60 -1.6 1,334,141 + Pf6N2E2_1137 0.67 +0.6 1,334,142 - Pf6N2E2_1137 0.67 +0.4 1,334,142 - Pf6N2E2_1137 0.67 -0.9 1,334,142 - Pf6N2E2_1137 0.67 -2.8 1,334,142 - Pf6N2E2_1137 0.67 -0.6 1,334,186 + Pf6N2E2_1137 0.71 +0.5 1,334,186 + Pf6N2E2_1137 0.71 +0.6 1,334,186 + Pf6N2E2_1137 0.71 +0.6 1,334,186 + Pf6N2E2_1137 0.71 +0.4 1,334,186 + Pf6N2E2_1137 0.71 +0.6 1,334,193 - Pf6N2E2_1137 0.71 -2.1 1,334,265 + Pf6N2E2_1137 0.77 -1.6 1,334,266 - Pf6N2E2_1137 0.77 -3.6 1,334,266 - Pf6N2E2_1137 0.77 -0.9 1,334,266 - Pf6N2E2_1137 0.77 -1.0 1,334,288 + Pf6N2E2_1137 0.78 +0.8 1,334,288 + Pf6N2E2_1137 0.78 -2.3 1,334,288 + Pf6N2E2_1137 0.78 -1.0 1,334,288 + Pf6N2E2_1137 0.78 -0.7 1,334,289 - Pf6N2E2_1137 0.78 -1.0 1,334,289 - Pf6N2E2_1137 0.78 -1.9 1,334,289 - Pf6N2E2_1137 0.78 -0.8 1,334,289 - Pf6N2E2_1137 0.78 -0.5 1,334,289 - Pf6N2E2_1137 0.78 -0.9 1,334,289 - Pf6N2E2_1137 0.78 -0.7 1,334,289 - Pf6N2E2_1137 0.78 -1.5 1,334,289 - Pf6N2E2_1137 0.78 +0.6 1,334,448 - -0.9 1,334,474 + +1.0 1,334,607 + -0.7 1,334,607 + -1.0 1,334,607 + -0.7 1,334,608 - -1.2 1,334,608 - -0.9 1,334,675 + -2.0 1,334,676 - -1.4 1,334,687 + Pf6N2E2_1138 0.11 -0.7 1,334,687 + Pf6N2E2_1138 0.11 -1.6 1,334,687 + Pf6N2E2_1138 0.11 -1.2 1,334,743 + Pf6N2E2_1138 0.16 -0.7 1,334,744 - Pf6N2E2_1138 0.16 -1.9 1,335,004 + Pf6N2E2_1138 0.42 -1.8 1,335,004 + Pf6N2E2_1138 0.42 -0.7 1,335,005 - Pf6N2E2_1138 0.42 -0.5 1,335,005 - Pf6N2E2_1138 0.42 -1.1 1,335,013 + Pf6N2E2_1138 0.42 -0.7 1,335,013 + Pf6N2E2_1138 0.42 -1.0 1,335,014 - Pf6N2E2_1138 0.43 -1.1 1,335,533 - -1.2 1,335,533 - -0.3 1,335,661 + +0.7 1,335,674 + +0.2 1,335,675 - +0.1 1,335,727 + +1.5 1,335,727 + +2.8 1,335,727 + +0.7 1,335,728 - -1.3 1,335,728 - -0.3 1,335,728 - +0.1 1,335,728 - -0.3 1,335,733 - +0.4 1,335,758 + -0.3 1,335,759 - +0.7 1,335,865 - Pf6N2E2_1139 0.11 +1.0 1,335,867 + Pf6N2E2_1139 0.11 +0.8 1,335,867 + Pf6N2E2_1139 0.11 -1.1 1,335,868 - Pf6N2E2_1139 0.11 -0.2 1,335,868 - Pf6N2E2_1139 0.11 -0.1 1,336,053 + Pf6N2E2_1139 0.30 +0.4 1,336,054 - Pf6N2E2_1139 0.30 +0.6 1,336,072 + Pf6N2E2_1139 0.32 +0.7 1,336,072 + Pf6N2E2_1139 0.32 +1.9 1,336,073 - Pf6N2E2_1139 0.32 -0.1 1,336,174 - Pf6N2E2_1139 0.43 +0.5 1,336,266 + Pf6N2E2_1139 0.52 +0.9 1,336,305 + Pf6N2E2_1139 0.56 -0.5 1,336,305 + Pf6N2E2_1139 0.56 +0.2 1,336,306 - Pf6N2E2_1139 0.56 -0.6 1,336,306 - Pf6N2E2_1139 0.56 -0.2 1,336,416 + Pf6N2E2_1139 0.68 -0.4 1,336,417 - Pf6N2E2_1139 0.68 -0.9 1,336,417 - Pf6N2E2_1139 0.68 -0.2 1,336,417 - Pf6N2E2_1139 0.68 +0.0 1,336,417 - Pf6N2E2_1139 0.68 -0.6 1,336,417 - Pf6N2E2_1139 0.68 -0.3 1,336,418 + Pf6N2E2_1139 0.68 +0.7 1,336,497 + Pf6N2E2_1139 0.76 +1.2 1,336,498 - Pf6N2E2_1139 0.76 -0.4 1,336,498 - Pf6N2E2_1139 0.76 +0.4 1,336,498 - Pf6N2E2_1139 0.76 -0.1 1,336,498 - Pf6N2E2_1139 0.76 -0.8
Or see this region's nucleotide sequence