Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1135 and Pf6N2E2_1136 are separated by 45 nucleotides Pf6N2E2_1136 and Pf6N2E2_1137 are separated by 121 nucleotides Pf6N2E2_1137 and Pf6N2E2_1138 overlap by 4 nucleotides
Pf6N2E2_1135 - COGs COG3558, at 1,332,025 to 1,332,504
_1135
Pf6N2E2_1136 - Transcriptional regulator, TetR family, at 1,332,550 to 1,333,113
_1136
Pf6N2E2_1137 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 1,333,235 to 1,334,578
_1137
Pf6N2E2_1138 - Transcriptional regulator, AraC family, at 1,334,575 to 1,335,606
_1138
Position (kb)
1333
1334
1335 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1332.713 kb on + strand, within Pf6N2E2_1136 at 1332.978 kb on - strand, within Pf6N2E2_1136 at 1333.048 kb on - strand, within Pf6N2E2_1136 at 1333.194 kb on + strand at 1333.194 kb on + strand at 1333.195 kb on - strand at 1333.195 kb on - strand at 1333.234 kb on + strand at 1333.235 kb on - strand at 1333.235 kb on - strand at 1333.376 kb on + strand, within Pf6N2E2_1137 at 1333.376 kb on + strand, within Pf6N2E2_1137 at 1333.377 kb on - strand, within Pf6N2E2_1137 at 1333.377 kb on - strand, within Pf6N2E2_1137 at 1333.377 kb on - strand, within Pf6N2E2_1137 at 1333.391 kb on + strand, within Pf6N2E2_1137 at 1333.392 kb on - strand, within Pf6N2E2_1137 at 1333.589 kb on + strand, within Pf6N2E2_1137 at 1333.590 kb on - strand, within Pf6N2E2_1137 at 1333.590 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.688 kb on - strand, within Pf6N2E2_1137 at 1333.694 kb on + strand, within Pf6N2E2_1137 at 1333.890 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1333.899 kb on - strand, within Pf6N2E2_1137 at 1334.039 kb on + strand, within Pf6N2E2_1137 at 1334.039 kb on + strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137 at 1334.040 kb on - strand, within Pf6N2E2_1137 at 1334.141 kb on + strand, within Pf6N2E2_1137 at 1334.142 kb on - strand, within Pf6N2E2_1137 at 1334.142 kb on - strand, within Pf6N2E2_1137 at 1334.142 kb on - strand, within Pf6N2E2_1137 at 1334.142 kb on - strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.186 kb on + strand, within Pf6N2E2_1137 at 1334.193 kb on - strand, within Pf6N2E2_1137 at 1334.265 kb on + strand, within Pf6N2E2_1137 at 1334.266 kb on - strand, within Pf6N2E2_1137 at 1334.266 kb on - strand, within Pf6N2E2_1137 at 1334.266 kb on - strand, within Pf6N2E2_1137 at 1334.288 kb on + strand, within Pf6N2E2_1137 at 1334.288 kb on + strand, within Pf6N2E2_1137 at 1334.288 kb on + strand, within Pf6N2E2_1137 at 1334.288 kb on + strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.289 kb on - strand, within Pf6N2E2_1137 at 1334.448 kb on - strand at 1334.474 kb on + strand at 1334.607 kb on + strand at 1334.607 kb on + strand at 1334.607 kb on + strand at 1334.608 kb on - strand at 1334.608 kb on - strand at 1334.675 kb on + strand at 1334.676 kb on - strand at 1334.687 kb on + strand, within Pf6N2E2_1138 at 1334.687 kb on + strand, within Pf6N2E2_1138 at 1334.687 kb on + strand, within Pf6N2E2_1138 at 1334.743 kb on + strand, within Pf6N2E2_1138 at 1334.744 kb on - strand, within Pf6N2E2_1138 at 1335.004 kb on + strand, within Pf6N2E2_1138 at 1335.004 kb on + strand, within Pf6N2E2_1138 at 1335.005 kb on - strand, within Pf6N2E2_1138 at 1335.005 kb on - strand, within Pf6N2E2_1138 at 1335.013 kb on + strand, within Pf6N2E2_1138 at 1335.013 kb on + strand, within Pf6N2E2_1138 at 1335.014 kb on - strand, within Pf6N2E2_1138 at 1335.533 kb on - strand at 1335.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,332,713 + Pf6N2E2_1136 0.29 +1.8 1,332,978 - Pf6N2E2_1136 0.76 -0.5 1,333,048 - Pf6N2E2_1136 0.88 +0.2 1,333,194 + +0.1 1,333,194 + +0.1 1,333,195 - -1.2 1,333,195 - -2.3 1,333,234 + +1.9 1,333,235 - -1.4 1,333,235 - -0.3 1,333,376 + Pf6N2E2_1137 0.10 +0.4 1,333,376 + Pf6N2E2_1137 0.10 -0.3 1,333,377 - Pf6N2E2_1137 0.11 -2.2 1,333,377 - Pf6N2E2_1137 0.11 -0.8 1,333,377 - Pf6N2E2_1137 0.11 -1.3 1,333,391 + Pf6N2E2_1137 0.12 +0.2 1,333,392 - Pf6N2E2_1137 0.12 -0.6 1,333,589 + Pf6N2E2_1137 0.26 +2.5 1,333,590 - Pf6N2E2_1137 0.26 -1.1 1,333,590 - Pf6N2E2_1137 0.26 -2.1 1,333,688 - Pf6N2E2_1137 0.34 -1.0 1,333,688 - Pf6N2E2_1137 0.34 -0.8 1,333,688 - Pf6N2E2_1137 0.34 -1.8 1,333,694 + Pf6N2E2_1137 0.34 +0.9 1,333,890 - Pf6N2E2_1137 0.49 -0.9 1,333,899 - Pf6N2E2_1137 0.49 -1.4 1,333,899 - Pf6N2E2_1137 0.49 -0.9 1,333,899 - Pf6N2E2_1137 0.49 -0.9 1,333,899 - Pf6N2E2_1137 0.49 +0.1 1,334,039 + Pf6N2E2_1137 0.60 +1.8 1,334,039 + Pf6N2E2_1137 0.60 -0.4 1,334,040 - Pf6N2E2_1137 0.60 -2.6 1,334,040 - Pf6N2E2_1137 0.60 +0.6 1,334,040 - Pf6N2E2_1137 0.60 -1.6 1,334,141 + Pf6N2E2_1137 0.67 +0.6 1,334,142 - Pf6N2E2_1137 0.67 +0.4 1,334,142 - Pf6N2E2_1137 0.67 -0.9 1,334,142 - Pf6N2E2_1137 0.67 -2.8 1,334,142 - Pf6N2E2_1137 0.67 -0.6 1,334,186 + Pf6N2E2_1137 0.71 +0.5 1,334,186 + Pf6N2E2_1137 0.71 +0.6 1,334,186 + Pf6N2E2_1137 0.71 +0.6 1,334,186 + Pf6N2E2_1137 0.71 +0.4 1,334,186 + Pf6N2E2_1137 0.71 +0.6 1,334,193 - Pf6N2E2_1137 0.71 -2.1 1,334,265 + Pf6N2E2_1137 0.77 -1.6 1,334,266 - Pf6N2E2_1137 0.77 -3.6 1,334,266 - Pf6N2E2_1137 0.77 -0.9 1,334,266 - Pf6N2E2_1137 0.77 -1.0 1,334,288 + Pf6N2E2_1137 0.78 +0.8 1,334,288 + Pf6N2E2_1137 0.78 -2.3 1,334,288 + Pf6N2E2_1137 0.78 -1.0 1,334,288 + Pf6N2E2_1137 0.78 -0.7 1,334,289 - Pf6N2E2_1137 0.78 -1.0 1,334,289 - Pf6N2E2_1137 0.78 -1.9 1,334,289 - Pf6N2E2_1137 0.78 -0.8 1,334,289 - Pf6N2E2_1137 0.78 -0.5 1,334,289 - Pf6N2E2_1137 0.78 -0.9 1,334,289 - Pf6N2E2_1137 0.78 -0.7 1,334,289 - Pf6N2E2_1137 0.78 -1.5 1,334,289 - Pf6N2E2_1137 0.78 +0.6 1,334,448 - -0.9 1,334,474 + +1.0 1,334,607 + -0.7 1,334,607 + -1.0 1,334,607 + -0.7 1,334,608 - -1.2 1,334,608 - -0.9 1,334,675 + -2.0 1,334,676 - -1.4 1,334,687 + Pf6N2E2_1138 0.11 -0.7 1,334,687 + Pf6N2E2_1138 0.11 -1.6 1,334,687 + Pf6N2E2_1138 0.11 -1.2 1,334,743 + Pf6N2E2_1138 0.16 -0.7 1,334,744 - Pf6N2E2_1138 0.16 -1.9 1,335,004 + Pf6N2E2_1138 0.42 -1.8 1,335,004 + Pf6N2E2_1138 0.42 -0.7 1,335,005 - Pf6N2E2_1138 0.42 -0.5 1,335,005 - Pf6N2E2_1138 0.42 -1.1 1,335,013 + Pf6N2E2_1138 0.42 -0.7 1,335,013 + Pf6N2E2_1138 0.42 -1.0 1,335,014 - Pf6N2E2_1138 0.43 -1.1 1,335,533 - -1.2 1,335,533 - -0.3
Or see this region's nucleotide sequence