Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1022

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1021 and Pf6N2E2_1022 are separated by 8 nucleotidesPf6N2E2_1022 and Pf6N2E2_1023 are separated by 50 nucleotides Pf6N2E2_1021 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,193,879 to 1,194,826 _1021 Pf6N2E2_1022 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,194,835 to 1,196,022 _1022 Pf6N2E2_1023 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,196,073 to 1,197,065 _1023 Position (kb) 1194 1195 1196 1197Strain fitness (log2 ratio) -2 -1 0 1at 1193.883 kb on + strandat 1193.895 kb on - strandat 1193.895 kb on - strandat 1193.998 kb on + strand, within Pf6N2E2_1021at 1193.999 kb on - strand, within Pf6N2E2_1021at 1193.999 kb on - strand, within Pf6N2E2_1021at 1194.030 kb on + strand, within Pf6N2E2_1021at 1194.030 kb on + strand, within Pf6N2E2_1021at 1194.030 kb on + strand, within Pf6N2E2_1021at 1194.031 kb on - strand, within Pf6N2E2_1021at 1194.031 kb on - strand, within Pf6N2E2_1021at 1194.409 kb on + strand, within Pf6N2E2_1021at 1194.409 kb on + strand, within Pf6N2E2_1021at 1194.409 kb on + strand, within Pf6N2E2_1021at 1194.410 kb on - strand, within Pf6N2E2_1021at 1194.410 kb on - strand, within Pf6N2E2_1021at 1194.410 kb on - strand, within Pf6N2E2_1021at 1194.459 kb on - strand, within Pf6N2E2_1021at 1194.583 kb on - strand, within Pf6N2E2_1021at 1194.628 kb on - strand, within Pf6N2E2_1021at 1195.244 kb on + strand, within Pf6N2E2_1022at 1195.366 kb on + strand, within Pf6N2E2_1022at 1195.396 kb on + strand, within Pf6N2E2_1022at 1195.733 kb on + strand, within Pf6N2E2_1022at 1195.913 kb on + strandat 1195.913 kb on + strandat 1195.914 kb on - strandat 1195.914 kb on - strandat 1195.914 kb on - strandat 1195.914 kb on - strandat 1196.005 kb on + strandat 1196.005 kb on + strandat 1196.006 kb on - strandat 1196.046 kb on + strandat 1196.057 kb on + strandat 1196.057 kb on + strandat 1196.233 kb on + strand, within Pf6N2E2_1023at 1196.233 kb on + strand, within Pf6N2E2_1023at 1196.233 kb on + strand, within Pf6N2E2_1023at 1196.233 kb on + strand, within Pf6N2E2_1023at 1196.233 kb on + strand, within Pf6N2E2_1023at 1196.233 kb on + strand, within Pf6N2E2_1023at 1196.233 kb on + strand, within Pf6N2E2_1023at 1196.234 kb on - strand, within Pf6N2E2_1023at 1196.234 kb on - strand, within Pf6N2E2_1023at 1196.234 kb on - strand, within Pf6N2E2_1023at 1196.234 kb on - strand, within Pf6N2E2_1023at 1196.344 kb on + strand, within Pf6N2E2_1023at 1196.344 kb on + strand, within Pf6N2E2_1023at 1196.344 kb on + strand, within Pf6N2E2_1023at 1196.407 kb on + strand, within Pf6N2E2_1023at 1196.407 kb on + strand, within Pf6N2E2_1023at 1196.408 kb on - strand, within Pf6N2E2_1023at 1196.488 kb on + strand, within Pf6N2E2_1023at 1196.488 kb on + strand, within Pf6N2E2_1023at 1196.488 kb on + strand, within Pf6N2E2_1023at 1196.489 kb on - strand, within Pf6N2E2_1023at 1196.869 kb on + strand, within Pf6N2E2_1023at 1196.923 kb on + strand, within Pf6N2E2_1023

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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1,193,883 + +0.1
1,193,895 - -0.5
1,193,895 - -0.8
1,193,998 + Pf6N2E2_1021 0.13 -0.1
1,193,999 - Pf6N2E2_1021 0.13 +0.5
1,193,999 - Pf6N2E2_1021 0.13 +0.5
1,194,030 + Pf6N2E2_1021 0.16 -0.7
1,194,030 + Pf6N2E2_1021 0.16 -0.4
1,194,030 + Pf6N2E2_1021 0.16 -0.1
1,194,031 - Pf6N2E2_1021 0.16 +0.0
1,194,031 - Pf6N2E2_1021 0.16 -0.0
1,194,409 + Pf6N2E2_1021 0.56 +0.6
1,194,409 + Pf6N2E2_1021 0.56 +0.3
1,194,409 + Pf6N2E2_1021 0.56 -1.0
1,194,410 - Pf6N2E2_1021 0.56 -0.1
1,194,410 - Pf6N2E2_1021 0.56 -0.8
1,194,410 - Pf6N2E2_1021 0.56 +0.2
1,194,459 - Pf6N2E2_1021 0.61 -0.1
1,194,583 - Pf6N2E2_1021 0.74 -1.0
1,194,628 - Pf6N2E2_1021 0.79 +1.0
1,195,244 + Pf6N2E2_1022 0.34 +0.3
1,195,366 + Pf6N2E2_1022 0.45 +0.1
1,195,396 + Pf6N2E2_1022 0.47 +0.6
1,195,733 + Pf6N2E2_1022 0.76 +1.0
1,195,913 + +0.3
1,195,913 + +0.1
1,195,914 - +0.1
1,195,914 - +0.2
1,195,914 - +0.5
1,195,914 - +0.9
1,196,005 + +0.3
1,196,005 + -0.5
1,196,006 - +0.7
1,196,046 + -0.9
1,196,057 + -0.5
1,196,057 + +1.1
1,196,233 + Pf6N2E2_1023 0.16 +0.2
1,196,233 + Pf6N2E2_1023 0.16 +0.4
1,196,233 + Pf6N2E2_1023 0.16 +0.8
1,196,233 + Pf6N2E2_1023 0.16 +0.5
1,196,233 + Pf6N2E2_1023 0.16 -0.9
1,196,233 + Pf6N2E2_1023 0.16 -0.8
1,196,233 + Pf6N2E2_1023 0.16 +0.2
1,196,234 - Pf6N2E2_1023 0.16 +1.5
1,196,234 - Pf6N2E2_1023 0.16 -2.1
1,196,234 - Pf6N2E2_1023 0.16 +0.4
1,196,234 - Pf6N2E2_1023 0.16 -0.4
1,196,344 + Pf6N2E2_1023 0.27 +0.5
1,196,344 + Pf6N2E2_1023 0.27 -0.4
1,196,344 + Pf6N2E2_1023 0.27 +0.2
1,196,407 + Pf6N2E2_1023 0.34 +0.6
1,196,407 + Pf6N2E2_1023 0.34 +0.1
1,196,408 - Pf6N2E2_1023 0.34 +0.2
1,196,488 + Pf6N2E2_1023 0.42 +0.3
1,196,488 + Pf6N2E2_1023 0.42 +0.4
1,196,488 + Pf6N2E2_1023 0.42 -0.3
1,196,489 - Pf6N2E2_1023 0.42 +0.1
1,196,869 + Pf6N2E2_1023 0.80 -0.9
1,196,923 + Pf6N2E2_1023 0.86 +0.6

Or see this region's nucleotide sequence