Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1021 and Pf6N2E2_1022 are separated by 8 nucleotides Pf6N2E2_1022 and Pf6N2E2_1023 are separated by 50 nucleotides
Pf6N2E2_1021 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,193,879 to 1,194,826
_1021
Pf6N2E2_1022 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,194,835 to 1,196,022
_1022
Pf6N2E2_1023 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,196,073 to 1,197,065
_1023
Position (kb)
1194
1195
1196
1197 Strain fitness (log2 ratio)
-2
-1
0
1 at 1193.883 kb on + strand at 1193.895 kb on - strand at 1193.895 kb on - strand at 1193.998 kb on + strand, within Pf6N2E2_1021 at 1193.999 kb on - strand, within Pf6N2E2_1021 at 1193.999 kb on - strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.031 kb on - strand, within Pf6N2E2_1021 at 1194.031 kb on - strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.459 kb on - strand, within Pf6N2E2_1021 at 1194.583 kb on - strand, within Pf6N2E2_1021 at 1194.628 kb on - strand, within Pf6N2E2_1021 at 1195.244 kb on + strand, within Pf6N2E2_1022 at 1195.366 kb on + strand, within Pf6N2E2_1022 at 1195.396 kb on + strand, within Pf6N2E2_1022 at 1195.733 kb on + strand, within Pf6N2E2_1022 at 1195.913 kb on + strand at 1195.913 kb on + strand at 1195.914 kb on - strand at 1195.914 kb on - strand at 1195.914 kb on - strand at 1195.914 kb on - strand at 1196.005 kb on + strand at 1196.005 kb on + strand at 1196.006 kb on - strand at 1196.046 kb on + strand at 1196.057 kb on + strand at 1196.057 kb on + strand at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.407 kb on + strand, within Pf6N2E2_1023 at 1196.407 kb on + strand, within Pf6N2E2_1023 at 1196.408 kb on - strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.489 kb on - strand, within Pf6N2E2_1023 at 1196.869 kb on + strand, within Pf6N2E2_1023 at 1196.923 kb on + strand, within Pf6N2E2_1023
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,193,883 + +0.1 1,193,895 - -0.5 1,193,895 - -0.8 1,193,998 + Pf6N2E2_1021 0.13 -0.1 1,193,999 - Pf6N2E2_1021 0.13 +0.5 1,193,999 - Pf6N2E2_1021 0.13 +0.5 1,194,030 + Pf6N2E2_1021 0.16 -0.7 1,194,030 + Pf6N2E2_1021 0.16 -0.4 1,194,030 + Pf6N2E2_1021 0.16 -0.1 1,194,031 - Pf6N2E2_1021 0.16 +0.0 1,194,031 - Pf6N2E2_1021 0.16 -0.0 1,194,409 + Pf6N2E2_1021 0.56 +0.6 1,194,409 + Pf6N2E2_1021 0.56 +0.3 1,194,409 + Pf6N2E2_1021 0.56 -1.0 1,194,410 - Pf6N2E2_1021 0.56 -0.1 1,194,410 - Pf6N2E2_1021 0.56 -0.8 1,194,410 - Pf6N2E2_1021 0.56 +0.2 1,194,459 - Pf6N2E2_1021 0.61 -0.1 1,194,583 - Pf6N2E2_1021 0.74 -1.0 1,194,628 - Pf6N2E2_1021 0.79 +1.0 1,195,244 + Pf6N2E2_1022 0.34 +0.3 1,195,366 + Pf6N2E2_1022 0.45 +0.1 1,195,396 + Pf6N2E2_1022 0.47 +0.6 1,195,733 + Pf6N2E2_1022 0.76 +1.0 1,195,913 + +0.3 1,195,913 + +0.1 1,195,914 - +0.1 1,195,914 - +0.2 1,195,914 - +0.5 1,195,914 - +0.9 1,196,005 + +0.3 1,196,005 + -0.5 1,196,006 - +0.7 1,196,046 + -0.9 1,196,057 + -0.5 1,196,057 + +1.1 1,196,233 + Pf6N2E2_1023 0.16 +0.2 1,196,233 + Pf6N2E2_1023 0.16 +0.4 1,196,233 + Pf6N2E2_1023 0.16 +0.8 1,196,233 + Pf6N2E2_1023 0.16 +0.5 1,196,233 + Pf6N2E2_1023 0.16 -0.9 1,196,233 + Pf6N2E2_1023 0.16 -0.8 1,196,233 + Pf6N2E2_1023 0.16 +0.2 1,196,234 - Pf6N2E2_1023 0.16 +1.5 1,196,234 - Pf6N2E2_1023 0.16 -2.1 1,196,234 - Pf6N2E2_1023 0.16 +0.4 1,196,234 - Pf6N2E2_1023 0.16 -0.4 1,196,344 + Pf6N2E2_1023 0.27 +0.5 1,196,344 + Pf6N2E2_1023 0.27 -0.4 1,196,344 + Pf6N2E2_1023 0.27 +0.2 1,196,407 + Pf6N2E2_1023 0.34 +0.6 1,196,407 + Pf6N2E2_1023 0.34 +0.1 1,196,408 - Pf6N2E2_1023 0.34 +0.2 1,196,488 + Pf6N2E2_1023 0.42 +0.3 1,196,488 + Pf6N2E2_1023 0.42 +0.4 1,196,488 + Pf6N2E2_1023 0.42 -0.3 1,196,489 - Pf6N2E2_1023 0.42 +0.1 1,196,869 + Pf6N2E2_1023 0.80 -0.9 1,196,923 + Pf6N2E2_1023 0.86 +0.6
Or see this region's nucleotide sequence