Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_216

Experiment: D-Ribose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_215 and Pf1N1B4_216 are separated by 381 nucleotidesPf1N1B4_216 and Pf1N1B4_217 are separated by 132 nucleotides Pf1N1B4_215 - Pyoverdine chromophore precursor synthetase PvdL, at 267,867 to 275,390 _215 Pf1N1B4_216 - Sigma factor PvdS, controling pyoverdin biosynthesis, at 275,772 to 276,320 _216 Pf1N1B4_217 - Hypothetical protein PvdY @ Siderophore synthetase small component, acetyltransferase, at 276,453 to 277,466 _217 Position (kb) 275 276 277Strain fitness (log2 ratio) -2 -1 0 1 2 3at 274.888 kb on + strandat 274.888 kb on + strandat 274.888 kb on + strandat 275.134 kb on + strandat 275.161 kb on + strandat 275.162 kb on - strandat 275.563 kb on + strandat 275.564 kb on - strandat 275.564 kb on - strandat 275.564 kb on - strandat 275.606 kb on + strandat 275.607 kb on - strandat 275.714 kb on - strandat 275.749 kb on + strandat 275.750 kb on - strandat 275.813 kb on - strandat 275.967 kb on + strand, within Pf1N1B4_216at 275.967 kb on + strand, within Pf1N1B4_216at 275.967 kb on + strand, within Pf1N1B4_216at 275.967 kb on + strand, within Pf1N1B4_216at 275.967 kb on + strand, within Pf1N1B4_216at 275.968 kb on - strand, within Pf1N1B4_216at 276.177 kb on + strand, within Pf1N1B4_216at 276.177 kb on + strandat 276.346 kb on + strandat 276.363 kb on + strandat 276.363 kb on + strandat 276.364 kb on - strandat 276.368 kb on + strandat 276.368 kb on + strandat 276.368 kb on + strandat 276.369 kb on - strandat 276.392 kb on - strandat 276.392 kb on - strandat 276.640 kb on + strand, within Pf1N1B4_217at 276.641 kb on - strand, within Pf1N1B4_217at 276.641 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strandat 276.743 kb on - strand, within Pf1N1B4_217at 276.743 kb on - strandat 276.743 kb on - strand, within Pf1N1B4_217at 276.775 kb on + strand, within Pf1N1B4_217at 276.776 kb on - strand, within Pf1N1B4_217at 276.776 kb on - strand, within Pf1N1B4_217at 276.787 kb on + strand, within Pf1N1B4_217at 276.788 kb on - strand, within Pf1N1B4_217at 276.788 kb on - strand, within Pf1N1B4_217at 276.831 kb on - strandat 276.831 kb on - strand, within Pf1N1B4_217at 276.860 kb on - strand, within Pf1N1B4_217at 276.961 kb on - strand, within Pf1N1B4_217at 277.081 kb on + strand, within Pf1N1B4_217

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Ribose (C)
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274,888 + +0.7
274,888 + -0.4
274,888 + +0.1
275,134 + +0.3
275,161 + -0.6
275,162 - -2.1
275,563 + +0.7
275,564 - +0.3
275,564 - -0.3
275,564 - -0.1
275,606 + +1.0
275,607 - -1.8
275,714 - +2.3
275,749 + +1.0
275,750 - +1.3
275,813 - -0.0
275,967 + Pf1N1B4_216 0.36 +0.5
275,967 + Pf1N1B4_216 0.36 -0.6
275,967 + Pf1N1B4_216 0.36 -1.0
275,967 + Pf1N1B4_216 0.36 -1.0
275,967 + Pf1N1B4_216 0.36 -0.6
275,968 - Pf1N1B4_216 0.36 -0.6
276,177 + Pf1N1B4_216 0.74 -1.6
276,177 + -1.0
276,346 + -0.6
276,363 + -0.6
276,363 + +0.8
276,364 - +3.0
276,368 + -1.0
276,368 + -1.1
276,368 + +0.5
276,369 - -1.3
276,392 - +0.3
276,392 - -0.9
276,640 + Pf1N1B4_217 0.18 -0.3
276,641 - Pf1N1B4_217 0.19 -0.9
276,641 - Pf1N1B4_217 0.19 +0.1
276,743 - Pf1N1B4_217 0.29 -0.8
276,743 - Pf1N1B4_217 0.29 -1.0
276,743 - +1.0
276,743 - Pf1N1B4_217 0.29 +0.0
276,743 - +1.0
276,743 - Pf1N1B4_217 0.29 +0.3
276,775 + Pf1N1B4_217 0.32 +0.7
276,776 - Pf1N1B4_217 0.32 -0.6
276,776 - Pf1N1B4_217 0.32 -0.4
276,787 + Pf1N1B4_217 0.33 -0.2
276,788 - Pf1N1B4_217 0.33 +0.3
276,788 - Pf1N1B4_217 0.33 -0.3
276,831 - +1.3
276,831 - Pf1N1B4_217 0.37 +0.8
276,860 - Pf1N1B4_217 0.40 +1.2
276,961 - Pf1N1B4_217 0.50 -1.5
277,081 + Pf1N1B4_217 0.62 +0.3

Or see this region's nucleotide sequence