Experiment: D-Ribose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2012 and Pf1N1B4_2013 are separated by 47 nucleotides Pf1N1B4_2013 and Pf1N1B4_2014 are separated by 111 nucleotides Pf1N1B4_2014 and Pf1N1B4_2015 are separated by 99 nucleotides Pf1N1B4_2015 and Pf1N1B4_2016 are separated by 17 nucleotides
Pf1N1B4_2012 - Amino acid ABC transporter, permease protein, at 2,298,879 to 2,299,661
_2012
Pf1N1B4_2013 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 2,299,709 to 2,300,530
_2013
Pf1N1B4_2014 - Homocysteine S-methyltransferase (EC 2.1.1.10), at 2,300,642 to 2,301,541
_2014
Pf1N1B4_2015 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 2,301,641 to 2,302,309
_2015
Pf1N1B4_2016 - N-acetylglutamate synthase (EC 2.3.1.1), at 2,302,327 to 2,302,773
_2016
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2299.851 kb on + strand at 2299.989 kb on + strand, within Pf1N1B4_2013 at 2299.989 kb on + strand, within Pf1N1B4_2013 at 2300.070 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.079 kb on + strand, within Pf1N1B4_2013 at 2300.080 kb on - strand, within Pf1N1B4_2013 at 2300.080 kb on - strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.137 kb on + strand at 2300.137 kb on + strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.138 kb on - strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.181 kb on + strand, within Pf1N1B4_2013 at 2300.182 kb on - strand, within Pf1N1B4_2013 at 2300.182 kb on - strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.184 kb on + strand, within Pf1N1B4_2013 at 2300.185 kb on - strand, within Pf1N1B4_2013 at 2300.185 kb on - strand, within Pf1N1B4_2013 at 2300.351 kb on + strand, within Pf1N1B4_2013 at 2300.404 kb on - strand, within Pf1N1B4_2013 at 2300.580 kb on - strand at 2300.587 kb on + strand at 2300.587 kb on + strand at 2300.587 kb on + strand at 2300.588 kb on - strand at 2300.589 kb on + strand at 2300.590 kb on - strand at 2300.590 kb on - strand at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.783 kb on + strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.784 kb on - strand, within Pf1N1B4_2014 at 2300.785 kb on + strand, within Pf1N1B4_2014 at 2300.785 kb on + strand, within Pf1N1B4_2014 at 2300.786 kb on - strand, within Pf1N1B4_2014 at 2300.822 kb on + strand, within Pf1N1B4_2014 at 2300.843 kb on - strand, within Pf1N1B4_2014 at 2300.843 kb on - strand, within Pf1N1B4_2014 at 2301.434 kb on + strand, within Pf1N1B4_2014 at 2301.511 kb on + strand at 2301.511 kb on + strand at 2301.511 kb on + strand at 2301.512 kb on - strand at 2301.512 kb on - strand at 2301.512 kb on - strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.579 kb on + strand at 2301.580 kb on - strand at 2301.601 kb on + strand at 2301.601 kb on + strand at 2301.602 kb on - strand at 2301.602 kb on - strand at 2301.602 kb on - strand at 2301.632 kb on - strand at 2301.632 kb on - strand at 2301.840 kb on + strand, within Pf1N1B4_2015 at 2301.840 kb on + strand, within Pf1N1B4_2015 at 2301.841 kb on - strand, within Pf1N1B4_2015 at 2301.841 kb on - strand, within Pf1N1B4_2015 at 2301.921 kb on + strand, within Pf1N1B4_2015 at 2302.071 kb on + strand, within Pf1N1B4_2015 at 2302.071 kb on + strand, within Pf1N1B4_2015 at 2302.072 kb on - strand, within Pf1N1B4_2015 at 2302.072 kb on - strand, within Pf1N1B4_2015 at 2302.149 kb on + strand, within Pf1N1B4_2015 at 2302.149 kb on + strand, within Pf1N1B4_2015 at 2302.150 kb on - strand, within Pf1N1B4_2015 at 2302.328 kb on + strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.329 kb on - strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.330 kb on + strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.331 kb on - strand at 2302.379 kb on + strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.380 kb on - strand, within Pf1N1B4_2016 at 2302.458 kb on - strand, within Pf1N1B4_2016 at 2302.458 kb on - strand, within Pf1N1B4_2016 at 2302.529 kb on + strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016 at 2302.530 kb on - strand, within Pf1N1B4_2016
Per-strain Table
Position Strand Gene LocusTag Fraction D-Ribose (C) remove 2,299,851 + +1.7 2,299,989 + Pf1N1B4_2013 0.34 +0.8 2,299,989 + Pf1N1B4_2013 0.34 -0.3 2,300,070 + Pf1N1B4_2013 0.44 +0.7 2,300,079 + Pf1N1B4_2013 0.45 +0.3 2,300,079 + Pf1N1B4_2013 0.45 +0.5 2,300,079 + Pf1N1B4_2013 0.45 +2.1 2,300,079 + Pf1N1B4_2013 0.45 +1.2 2,300,079 + Pf1N1B4_2013 0.45 -0.0 2,300,080 - Pf1N1B4_2013 0.45 -0.1 2,300,080 - Pf1N1B4_2013 0.45 -0.4 2,300,137 + Pf1N1B4_2013 0.52 -0.4 2,300,137 + Pf1N1B4_2013 0.52 -1.0 2,300,137 + Pf1N1B4_2013 0.52 +0.8 2,300,137 + Pf1N1B4_2013 0.52 -0.1 2,300,137 + +1.7 2,300,137 + Pf1N1B4_2013 0.52 -0.5 2,300,138 - Pf1N1B4_2013 0.52 +1.0 2,300,138 - Pf1N1B4_2013 0.52 +0.3 2,300,138 - Pf1N1B4_2013 0.52 -0.9 2,300,138 - Pf1N1B4_2013 0.52 -0.3 2,300,181 + Pf1N1B4_2013 0.57 -0.5 2,300,181 + Pf1N1B4_2013 0.57 -0.3 2,300,181 + Pf1N1B4_2013 0.57 -0.7 2,300,181 + Pf1N1B4_2013 0.57 +2.4 2,300,182 - Pf1N1B4_2013 0.58 -1.1 2,300,182 - Pf1N1B4_2013 0.58 +0.4 2,300,184 + Pf1N1B4_2013 0.58 +0.1 2,300,184 + Pf1N1B4_2013 0.58 +1.7 2,300,184 + Pf1N1B4_2013 0.58 -0.7 2,300,184 + Pf1N1B4_2013 0.58 +1.4 2,300,185 - Pf1N1B4_2013 0.58 +0.1 2,300,185 - Pf1N1B4_2013 0.58 +0.3 2,300,351 + Pf1N1B4_2013 0.78 -0.7 2,300,404 - Pf1N1B4_2013 0.85 +0.0 2,300,580 - -0.4 2,300,587 + +0.9 2,300,587 + -0.7 2,300,587 + -0.3 2,300,588 - -0.5 2,300,589 + +2.7 2,300,590 - -0.7 2,300,590 - -1.1 2,300,783 + Pf1N1B4_2014 0.16 +0.3 2,300,783 + Pf1N1B4_2014 0.16 +0.2 2,300,783 + Pf1N1B4_2014 0.16 -0.3 2,300,784 - Pf1N1B4_2014 0.16 -0.7 2,300,784 - Pf1N1B4_2014 0.16 -0.7 2,300,784 - Pf1N1B4_2014 0.16 -1.3 2,300,785 + Pf1N1B4_2014 0.16 +0.1 2,300,785 + Pf1N1B4_2014 0.16 -0.9 2,300,786 - Pf1N1B4_2014 0.16 +0.1 2,300,822 + Pf1N1B4_2014 0.20 -0.7 2,300,843 - Pf1N1B4_2014 0.22 -1.8 2,300,843 - Pf1N1B4_2014 0.22 -0.5 2,301,434 + Pf1N1B4_2014 0.88 -0.5 2,301,511 + -0.2 2,301,511 + -0.3 2,301,511 + -0.5 2,301,512 - +0.3 2,301,512 - +0.4 2,301,512 - +0.3 2,301,579 + -0.3 2,301,579 + +0.3 2,301,579 + +1.7 2,301,579 + +0.3 2,301,579 + +0.7 2,301,580 - +1.4 2,301,601 + -1.3 2,301,601 + -0.6 2,301,602 - -0.8 2,301,602 - -0.1 2,301,602 - -0.3 2,301,632 - -0.1 2,301,632 - -0.9 2,301,840 + Pf1N1B4_2015 0.30 +0.7 2,301,840 + Pf1N1B4_2015 0.30 -1.1 2,301,841 - Pf1N1B4_2015 0.30 -0.2 2,301,841 - Pf1N1B4_2015 0.30 -0.5 2,301,921 + Pf1N1B4_2015 0.42 -0.8 2,302,071 + Pf1N1B4_2015 0.64 +0.7 2,302,071 + Pf1N1B4_2015 0.64 -1.0 2,302,072 - Pf1N1B4_2015 0.64 +1.1 2,302,072 - Pf1N1B4_2015 0.64 -0.1 2,302,149 + Pf1N1B4_2015 0.76 +0.4 2,302,149 + Pf1N1B4_2015 0.76 -0.7 2,302,150 - Pf1N1B4_2015 0.76 -0.8 2,302,328 + +0.9 2,302,329 - +1.7 2,302,329 - +2.0 2,302,329 - -1.5 2,302,329 - +0.9 2,302,329 - -1.4 2,302,329 - -0.2 2,302,329 - +1.0 2,302,329 - -0.1 2,302,330 + -0.7 2,302,330 + +0.4 2,302,330 + -1.3 2,302,330 + +0.2 2,302,331 - +0.7 2,302,331 - -0.8 2,302,331 - -0.1 2,302,331 - -0.4 2,302,331 - +0.1 2,302,331 - +0.1 2,302,331 - -0.5 2,302,331 - +0.9 2,302,331 - -1.8 2,302,379 + Pf1N1B4_2016 0.12 +0.0 2,302,380 - Pf1N1B4_2016 0.12 +1.7 2,302,380 - Pf1N1B4_2016 0.12 -2.1 2,302,380 - Pf1N1B4_2016 0.12 -0.7 2,302,458 - Pf1N1B4_2016 0.29 +0.3 2,302,458 - Pf1N1B4_2016 0.29 -0.2 2,302,529 + Pf1N1B4_2016 0.45 -1.9 2,302,530 - Pf1N1B4_2016 0.45 -0.2 2,302,530 - Pf1N1B4_2016 0.45 -0.3 2,302,530 - Pf1N1B4_2016 0.45 -0.4
Or see this region's nucleotide sequence