Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS00315 and HSERO_RS00320 are separated by 5 nucleotides HSERO_RS00320 and HSERO_RS00325 are separated by 60 nucleotides HSERO_RS00325 and HSERO_RS00330 overlap by 4 nucleotides HSERO_RS00330 and HSERO_RS00335 are separated by 128 nucleotides
HSERO_RS00315: HSERO_RS00315 - amino acid ABC transporter permease, at 77,496 to 78,248
_RS00315
HSERO_RS00320: HSERO_RS00320 - peptide ABC transporter ATP-binding protein, at 78,254 to 78,976
_RS00320
HSERO_RS00325: HSERO_RS00325 - multidrug DMT transporter permease, at 79,037 to 79,882
_RS00325
HSERO_RS00330: HSERO_RS00330 - SAM-dependent methyltransferase, at 79,879 to 80,556
_RS00330
HSERO_RS00335: HSERO_RS00335 - hypothetical protein, at 80,685 to 80,942
_RS00335
Position (kb)
79
80 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 78.103 kb on + strand, within HSERO_RS00315 at 78.106 kb on - strand, within HSERO_RS00315 at 78.264 kb on - strand at 78.264 kb on - strand at 78.302 kb on - strand at 78.466 kb on + strand, within HSERO_RS00320 at 78.474 kb on - strand, within HSERO_RS00320 at 78.720 kb on + strand, within HSERO_RS00320 at 78.764 kb on - strand, within HSERO_RS00320 at 78.845 kb on - strand, within HSERO_RS00320 at 78.857 kb on + strand, within HSERO_RS00320 at 78.875 kb on - strand, within HSERO_RS00320 at 79.035 kb on + strand at 79.035 kb on + strand at 79.124 kb on - strand, within HSERO_RS00325 at 79.260 kb on - strand, within HSERO_RS00325 at 79.332 kb on - strand, within HSERO_RS00325 at 79.407 kb on - strand, within HSERO_RS00325 at 79.507 kb on - strand, within HSERO_RS00325 at 79.533 kb on + strand, within HSERO_RS00325 at 79.533 kb on + strand, within HSERO_RS00325 at 79.541 kb on - strand, within HSERO_RS00325 at 79.627 kb on - strand, within HSERO_RS00325 at 79.671 kb on - strand, within HSERO_RS00325 at 79.676 kb on + strand, within HSERO_RS00325 at 79.717 kb on + strand at 79.725 kb on - strand, within HSERO_RS00325 at 79.809 kb on + strand at 79.809 kb on + strand at 79.809 kb on + strand at 79.856 kb on - strand at 79.959 kb on + strand, within HSERO_RS00330 at 80.050 kb on - strand at 80.172 kb on - strand, within HSERO_RS00330 at 80.240 kb on + strand, within HSERO_RS00330 at 80.264 kb on - strand, within HSERO_RS00330 at 80.430 kb on + strand, within HSERO_RS00330 at 80.464 kb on - strand, within HSERO_RS00330 at 80.475 kb on - strand, within HSERO_RS00330 at 80.524 kb on + strand at 80.532 kb on - strand at 80.539 kb on + strand at 80.681 kb on - strand at 80.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 78,103 + HSERO_RS00315 0.81 +0.1 78,106 - HSERO_RS00315 0.81 +0.3 78,264 - +0.7 78,264 - +0.1 78,302 - +0.9 78,466 + HSERO_RS00320 0.29 -0.8 78,474 - HSERO_RS00320 0.30 -0.3 78,720 + HSERO_RS00320 0.64 -0.3 78,764 - HSERO_RS00320 0.71 +0.1 78,845 - HSERO_RS00320 0.82 -0.2 78,857 + HSERO_RS00320 0.83 +0.4 78,875 - HSERO_RS00320 0.86 +0.4 79,035 + +0.2 79,035 + +0.0 79,124 - HSERO_RS00325 0.10 -0.2 79,260 - HSERO_RS00325 0.26 -1.3 79,332 - HSERO_RS00325 0.35 -0.1 79,407 - HSERO_RS00325 0.44 -0.1 79,507 - HSERO_RS00325 0.56 -0.5 79,533 + HSERO_RS00325 0.59 -0.7 79,533 + HSERO_RS00325 0.59 -3.0 79,541 - HSERO_RS00325 0.60 +0.6 79,627 - HSERO_RS00325 0.70 -0.1 79,671 - HSERO_RS00325 0.75 +0.0 79,676 + HSERO_RS00325 0.76 -1.3 79,717 + +0.9 79,725 - HSERO_RS00325 0.81 -0.5 79,809 + +0.2 79,809 + -0.1 79,809 + -0.4 79,856 - +0.3 79,959 + HSERO_RS00330 0.12 -0.5 80,050 - +0.9 80,172 - HSERO_RS00330 0.43 -0.4 80,240 + HSERO_RS00330 0.53 -1.0 80,264 - HSERO_RS00330 0.57 -0.4 80,430 + HSERO_RS00330 0.81 -0.1 80,464 - HSERO_RS00330 0.86 +0.1 80,475 - HSERO_RS00330 0.88 -1.4 80,524 + +0.6 80,532 - +0.0 80,539 + -1.0 80,681 - -1.4 80,691 - -0.2
Or see this region's nucleotide sequence