Experiment: D-Mannose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS07185 and HMPREF1058_RS07180 are separated by 21 nucleotides HMPREF1058_RS07180 and HMPREF1058_RS07175 are separated by 12 nucleotides HMPREF1058_RS07175 and HMPREF1058_RS07170 are separated by 60 nucleotides
HMPREF1058_RS07185: HMPREF1058_RS07185 - anthranilate phosphoribosyltransferase, at 292,442 to 293,437
_RS07185
HMPREF1058_RS07180: HMPREF1058_RS07180 - aminodeoxychorismate/anthranilate synthase component II, at 293,459 to 294,049
_RS07180
HMPREF1058_RS07175: HMPREF1058_RS07175 - anthranilate synthase component I family protein, at 294,062 to 295,462
_RS07175
HMPREF1058_RS07170: HMPREF1058_RS07170 - tryptophan synthase subunit beta, at 295,523 to 296,722
_RS07170
Position (kb)
294
295
296 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 293.106 kb on + strand, within HMPREF1058_RS07185 at 293.164 kb on + strand, within HMPREF1058_RS07185 at 293.180 kb on - strand, within HMPREF1058_RS07185 at 293.237 kb on + strand, within HMPREF1058_RS07185 at 293.346 kb on + strand at 293.445 kb on - strand at 293.445 kb on - strand at 293.448 kb on + strand at 293.448 kb on + strand at 293.551 kb on + strand, within HMPREF1058_RS07180 at 293.686 kb on - strand, within HMPREF1058_RS07180 at 293.744 kb on + strand, within HMPREF1058_RS07180 at 293.745 kb on - strand, within HMPREF1058_RS07180 at 293.756 kb on + strand, within HMPREF1058_RS07180 at 293.756 kb on + strand, within HMPREF1058_RS07180 at 293.820 kb on - strand, within HMPREF1058_RS07180 at 293.820 kb on - strand, within HMPREF1058_RS07180 at 293.820 kb on - strand, within HMPREF1058_RS07180 at 293.855 kb on - strand, within HMPREF1058_RS07180 at 293.960 kb on + strand, within HMPREF1058_RS07180 at 293.963 kb on + strand, within HMPREF1058_RS07180 at 294.014 kb on - strand at 294.025 kb on - strand at 294.025 kb on - strand at 294.025 kb on - strand at 294.025 kb on - strand at 294.039 kb on - strand at 294.125 kb on + strand at 294.148 kb on + strand at 294.149 kb on - strand at 294.153 kb on - strand at 294.205 kb on + strand, within HMPREF1058_RS07175 at 294.205 kb on + strand, within HMPREF1058_RS07175 at 294.206 kb on - strand, within HMPREF1058_RS07175 at 294.317 kb on + strand, within HMPREF1058_RS07175 at 294.318 kb on - strand, within HMPREF1058_RS07175 at 294.361 kb on + strand, within HMPREF1058_RS07175 at 294.362 kb on - strand, within HMPREF1058_RS07175 at 294.510 kb on - strand, within HMPREF1058_RS07175 at 294.518 kb on + strand, within HMPREF1058_RS07175 at 294.908 kb on - strand, within HMPREF1058_RS07175 at 294.912 kb on + strand, within HMPREF1058_RS07175 at 294.912 kb on + strand, within HMPREF1058_RS07175 at 294.913 kb on - strand, within HMPREF1058_RS07175 at 294.913 kb on - strand, within HMPREF1058_RS07175 at 294.913 kb on - strand, within HMPREF1058_RS07175 at 294.917 kb on - strand, within HMPREF1058_RS07175 at 295.027 kb on + strand, within HMPREF1058_RS07175 at 295.031 kb on + strand, within HMPREF1058_RS07175 at 295.037 kb on - strand, within HMPREF1058_RS07175 at 295.038 kb on + strand, within HMPREF1058_RS07175 at 295.039 kb on - strand, within HMPREF1058_RS07175 at 295.039 kb on - strand, within HMPREF1058_RS07175 at 295.082 kb on + strand, within HMPREF1058_RS07175 at 295.126 kb on - strand, within HMPREF1058_RS07175 at 295.397 kb on - strand at 295.408 kb on + strand at 295.446 kb on + strand at 295.447 kb on - strand at 295.480 kb on + strand at 295.481 kb on - strand at 295.561 kb on - strand at 295.607 kb on + strand at 295.607 kb on + strand at 295.607 kb on + strand at 295.608 kb on - strand at 295.829 kb on + strand, within HMPREF1058_RS07170 at 295.927 kb on + strand, within HMPREF1058_RS07170 at 295.977 kb on - strand, within HMPREF1058_RS07170 at 295.979 kb on - strand, within HMPREF1058_RS07170 at 295.980 kb on + strand, within HMPREF1058_RS07170 at 295.986 kb on + strand, within HMPREF1058_RS07170 at 296.019 kb on - strand, within HMPREF1058_RS07170 at 296.035 kb on - strand, within HMPREF1058_RS07170 at 296.117 kb on - strand, within HMPREF1058_RS07170 at 296.158 kb on - strand, within HMPREF1058_RS07170 at 296.158 kb on - strand, within HMPREF1058_RS07170 at 296.160 kb on + strand, within HMPREF1058_RS07170 at 296.160 kb on + strand, within HMPREF1058_RS07170 at 296.161 kb on - strand, within HMPREF1058_RS07170 at 296.284 kb on + strand, within HMPREF1058_RS07170 at 296.316 kb on + strand, within HMPREF1058_RS07170 at 296.316 kb on + strand, within HMPREF1058_RS07170 at 296.317 kb on - strand, within HMPREF1058_RS07170 at 296.413 kb on + strand, within HMPREF1058_RS07170
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose 20 mM (C) remove 293,106 + HMPREF1058_RS07185 0.67 -2.3 293,164 + HMPREF1058_RS07185 0.72 -1.6 293,180 - HMPREF1058_RS07185 0.74 -3.3 293,237 + HMPREF1058_RS07185 0.80 -2.6 293,346 + -4.6 293,445 - +0.6 293,445 - +2.2 293,448 + -4.4 293,448 + -4.4 293,551 + HMPREF1058_RS07180 0.16 -4.4 293,686 - HMPREF1058_RS07180 0.38 -1.0 293,744 + HMPREF1058_RS07180 0.48 -4.0 293,745 - HMPREF1058_RS07180 0.48 -1.3 293,756 + HMPREF1058_RS07180 0.50 -2.9 293,756 + HMPREF1058_RS07180 0.50 -1.5 293,820 - HMPREF1058_RS07180 0.61 -0.6 293,820 - HMPREF1058_RS07180 0.61 -4.1 293,820 - HMPREF1058_RS07180 0.61 -4.7 293,855 - HMPREF1058_RS07180 0.67 -4.5 293,960 + HMPREF1058_RS07180 0.85 -1.5 293,963 + HMPREF1058_RS07180 0.85 -1.8 294,014 - -2.8 294,025 - -2.8 294,025 - -3.5 294,025 - -4.8 294,025 - -3.9 294,039 - -2.5 294,125 + -3.6 294,148 + -2.6 294,149 - -1.3 294,153 - -2.5 294,205 + HMPREF1058_RS07175 0.10 -6.1 294,205 + HMPREF1058_RS07175 0.10 -2.6 294,206 - HMPREF1058_RS07175 0.10 -3.4 294,317 + HMPREF1058_RS07175 0.18 -4.5 294,318 - HMPREF1058_RS07175 0.18 -3.9 294,361 + HMPREF1058_RS07175 0.21 -3.4 294,362 - HMPREF1058_RS07175 0.21 -2.4 294,510 - HMPREF1058_RS07175 0.32 -4.5 294,518 + HMPREF1058_RS07175 0.33 -3.9 294,908 - HMPREF1058_RS07175 0.60 -4.3 294,912 + HMPREF1058_RS07175 0.61 -4.1 294,912 + HMPREF1058_RS07175 0.61 -3.3 294,913 - HMPREF1058_RS07175 0.61 -5.4 294,913 - HMPREF1058_RS07175 0.61 -3.7 294,913 - HMPREF1058_RS07175 0.61 -1.9 294,917 - HMPREF1058_RS07175 0.61 -3.8 295,027 + HMPREF1058_RS07175 0.69 -2.4 295,031 + HMPREF1058_RS07175 0.69 -2.6 295,037 - HMPREF1058_RS07175 0.70 -4.2 295,038 + HMPREF1058_RS07175 0.70 -0.9 295,039 - HMPREF1058_RS07175 0.70 -3.0 295,039 - HMPREF1058_RS07175 0.70 -3.6 295,082 + HMPREF1058_RS07175 0.73 -3.1 295,126 - HMPREF1058_RS07175 0.76 -6.8 295,397 - -1.9 295,408 + -3.9 295,446 + -3.5 295,447 - -1.3 295,480 + -0.6 295,481 - -0.9 295,561 - -1.8 295,607 + -6.5 295,607 + -3.7 295,607 + -3.6 295,608 - -1.3 295,829 + HMPREF1058_RS07170 0.26 -2.3 295,927 + HMPREF1058_RS07170 0.34 -2.7 295,977 - HMPREF1058_RS07170 0.38 -4.0 295,979 - HMPREF1058_RS07170 0.38 -3.2 295,980 + HMPREF1058_RS07170 0.38 -3.7 295,986 + HMPREF1058_RS07170 0.39 -3.4 296,019 - HMPREF1058_RS07170 0.41 -3.6 296,035 - HMPREF1058_RS07170 0.43 -4.8 296,117 - HMPREF1058_RS07170 0.49 -4.4 296,158 - HMPREF1058_RS07170 0.53 -1.9 296,158 - HMPREF1058_RS07170 0.53 -4.5 296,160 + HMPREF1058_RS07170 0.53 -2.3 296,160 + HMPREF1058_RS07170 0.53 -5.2 296,161 - HMPREF1058_RS07170 0.53 -4.7 296,284 + HMPREF1058_RS07170 0.63 -2.7 296,316 + HMPREF1058_RS07170 0.66 -2.6 296,316 + HMPREF1058_RS07170 0.66 -2.4 296,317 - HMPREF1058_RS07170 0.66 -3.7 296,413 + HMPREF1058_RS07170 0.74 -3.4
Or see this region's nucleotide sequence