Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37225 and RR42_RS37230 are separated by 316 nucleotides RR42_RS37230 and RR42_RS37235 are separated by 95 nucleotides
RR42_RS37225: RR42_RS37225 - hypothetical protein, at 3,817,122 to 3,817,388
_RS37225
RR42_RS37230: RR42_RS37230 - hypothetical protein, at 3,817,705 to 3,817,935
_RS37230
RR42_RS37235: RR42_RS37235 - hypothetical protein, at 3,818,031 to 3,818,282
_RS37235
Position (kb)
3817
3818 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3816.918 kb on + strand at 3816.918 kb on + strand at 3816.919 kb on - strand at 3816.919 kb on - strand at 3816.919 kb on - strand at 3816.931 kb on + strand at 3816.955 kb on - strand at 3816.963 kb on + strand at 3817.030 kb on + strand at 3817.030 kb on + strand at 3817.030 kb on + strand at 3817.031 kb on - strand at 3817.031 kb on - strand at 3817.031 kb on - strand at 3817.031 kb on - strand at 3817.051 kb on + strand at 3817.069 kb on - strand at 3817.070 kb on - strand at 3817.139 kb on + strand at 3817.139 kb on + strand at 3817.166 kb on - strand, within RR42_RS37225 at 3817.185 kb on - strand, within RR42_RS37225 at 3817.209 kb on + strand, within RR42_RS37225 at 3817.209 kb on + strand, within RR42_RS37225 at 3817.276 kb on + strand, within RR42_RS37225 at 3817.284 kb on + strand, within RR42_RS37225 at 3817.284 kb on + strand, within RR42_RS37225 at 3817.284 kb on + strand, within RR42_RS37225 at 3817.284 kb on + strand, within RR42_RS37225 at 3817.285 kb on - strand, within RR42_RS37225 at 3817.288 kb on - strand, within RR42_RS37225 at 3817.288 kb on - strand, within RR42_RS37225 at 3817.303 kb on + strand, within RR42_RS37225 at 3817.304 kb on - strand, within RR42_RS37225 at 3817.375 kb on + strand at 3817.375 kb on + strand at 3817.376 kb on - strand at 3817.376 kb on - strand at 3817.376 kb on - strand at 3817.381 kb on + strand at 3817.381 kb on + strand at 3817.381 kb on + strand at 3817.381 kb on + strand at 3817.382 kb on - strand at 3817.382 kb on - strand at 3817.386 kb on + strand at 3817.431 kb on + strand at 3817.431 kb on + strand at 3817.431 kb on + strand at 3817.432 kb on - strand at 3817.500 kb on + strand at 3817.501 kb on - strand at 3817.501 kb on - strand at 3817.524 kb on + strand at 3817.570 kb on + strand at 3817.571 kb on - strand at 3817.642 kb on + strand at 3817.643 kb on - strand at 3817.643 kb on - strand at 3817.643 kb on - strand at 3817.675 kb on + strand at 3817.676 kb on - strand at 3817.676 kb on - strand at 3817.691 kb on - strand at 3817.691 kb on - strand at 3817.691 kb on - strand at 3817.739 kb on + strand, within RR42_RS37230 at 3817.739 kb on + strand, within RR42_RS37230 at 3817.740 kb on - strand, within RR42_RS37230 at 3817.740 kb on - strand, within RR42_RS37230 at 3817.811 kb on + strand, within RR42_RS37230 at 3817.812 kb on - strand, within RR42_RS37230 at 3817.812 kb on - strand, within RR42_RS37230 at 3817.816 kb on - strand, within RR42_RS37230 at 3817.837 kb on + strand, within RR42_RS37230 at 3817.837 kb on + strand, within RR42_RS37230 at 3817.838 kb on - strand, within RR42_RS37230 at 3817.838 kb on - strand, within RR42_RS37230 at 3817.859 kb on + strand, within RR42_RS37230 at 3817.860 kb on - strand, within RR42_RS37230 at 3817.870 kb on + strand, within RR42_RS37230 at 3817.889 kb on - strand, within RR42_RS37230 at 3817.889 kb on - strand, within RR42_RS37230 at 3817.922 kb on - strand at 3817.931 kb on - strand at 3817.931 kb on - strand at 3817.935 kb on + strand at 3817.936 kb on - strand at 3817.936 kb on - strand at 3817.936 kb on - strand at 3817.936 kb on - strand at 3817.938 kb on + strand at 3817.939 kb on - strand at 3817.939 kb on - strand at 3817.939 kb on - strand at 3817.939 kb on - strand at 3818.044 kb on + strand at 3818.115 kb on + strand, within RR42_RS37235 at 3818.116 kb on - strand, within RR42_RS37235 at 3818.116 kb on - strand, within RR42_RS37235 at 3818.224 kb on + strand, within RR42_RS37235 at 3818.224 kb on + strand, within RR42_RS37235 at 3818.224 kb on + strand, within RR42_RS37235 at 3818.224 kb on + strand, within RR42_RS37235 at 3818.225 kb on - strand, within RR42_RS37235 at 3818.225 kb on - strand, within RR42_RS37235 at 3818.225 kb on - strand, within RR42_RS37235 at 3818.227 kb on + strand, within RR42_RS37235 at 3818.228 kb on - strand, within RR42_RS37235 at 3818.254 kb on + strand, within RR42_RS37235 at 3818.254 kb on + strand, within RR42_RS37235 at 3818.255 kb on - strand, within RR42_RS37235 at 3818.255 kb on - strand, within RR42_RS37235 at 3818.255 kb on - strand, within RR42_RS37235 at 3818.265 kb on - strand at 3818.336 kb on + strand at 3818.419 kb on - strand at 3818.482 kb on - strand at 3818.482 kb on - strand at 3818.538 kb on - strand at 3818.545 kb on + strand at 3818.610 kb on - strand at 3818.723 kb on + strand at 3818.763 kb on - strand at 3818.776 kb on + strand at 3818.776 kb on + strand at 3818.776 kb on + strand at 3818.776 kb on + strand at 3818.777 kb on - strand at 3818.777 kb on - strand at 3818.777 kb on - strand at 3818.779 kb on + strand at 3818.926 kb on + strand at 3818.926 kb on + strand at 3818.926 kb on + strand at 3818.926 kb on + strand at 3818.926 kb on + strand at 3818.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,816,918 + +0.8 3,816,918 + -1.4 3,816,919 - -0.4 3,816,919 - +0.2 3,816,919 - -1.4 3,816,931 + +0.2 3,816,955 - -0.8 3,816,963 + -0.4 3,817,030 + -3.0 3,817,030 + -1.5 3,817,030 + -0.7 3,817,031 - +0.4 3,817,031 - -0.9 3,817,031 - -2.1 3,817,031 - +1.2 3,817,051 + +0.1 3,817,069 - -0.8 3,817,070 - +1.2 3,817,139 + +0.5 3,817,139 + +0.5 3,817,166 - RR42_RS37225 0.16 +1.2 3,817,185 - RR42_RS37225 0.24 +1.2 3,817,209 + RR42_RS37225 0.33 +0.7 3,817,209 + RR42_RS37225 0.33 -0.6 3,817,276 + RR42_RS37225 0.58 -0.7 3,817,284 + RR42_RS37225 0.61 -1.7 3,817,284 + RR42_RS37225 0.61 +0.2 3,817,284 + RR42_RS37225 0.61 -1.4 3,817,284 + RR42_RS37225 0.61 +2.5 3,817,285 - RR42_RS37225 0.61 -2.2 3,817,288 - RR42_RS37225 0.62 +0.2 3,817,288 - RR42_RS37225 0.62 +1.4 3,817,303 + RR42_RS37225 0.68 -0.7 3,817,304 - RR42_RS37225 0.68 +0.3 3,817,375 + +0.7 3,817,375 + -1.8 3,817,376 - -1.8 3,817,376 - +0.5 3,817,376 - +0.1 3,817,381 + -0.5 3,817,381 + +0.4 3,817,381 + +1.7 3,817,381 + -0.0 3,817,382 - +1.0 3,817,382 - +1.0 3,817,386 + -0.7 3,817,431 + -0.7 3,817,431 + +0.5 3,817,431 + -1.6 3,817,432 - -0.7 3,817,500 + +0.4 3,817,501 - -1.3 3,817,501 - -1.0 3,817,524 + -1.5 3,817,570 + +0.6 3,817,571 - +0.2 3,817,642 + -0.1 3,817,643 - -0.0 3,817,643 - -0.4 3,817,643 - +1.4 3,817,675 + -0.3 3,817,676 - +0.1 3,817,676 - +0.3 3,817,691 - +0.8 3,817,691 - -1.5 3,817,691 - -0.1 3,817,739 + RR42_RS37230 0.15 -2.4 3,817,739 + RR42_RS37230 0.15 +1.4 3,817,740 - RR42_RS37230 0.15 +0.5 3,817,740 - RR42_RS37230 0.15 -0.2 3,817,811 + RR42_RS37230 0.46 -0.0 3,817,812 - RR42_RS37230 0.46 -0.2 3,817,812 - RR42_RS37230 0.46 +1.2 3,817,816 - RR42_RS37230 0.48 +0.5 3,817,837 + RR42_RS37230 0.57 -0.5 3,817,837 + RR42_RS37230 0.57 +0.1 3,817,838 - RR42_RS37230 0.58 +3.2 3,817,838 - RR42_RS37230 0.58 +0.6 3,817,859 + RR42_RS37230 0.67 +1.6 3,817,860 - RR42_RS37230 0.67 -0.4 3,817,870 + RR42_RS37230 0.71 -0.7 3,817,889 - RR42_RS37230 0.80 -3.4 3,817,889 - RR42_RS37230 0.80 -0.4 3,817,922 - +0.4 3,817,931 - -0.5 3,817,931 - -0.7 3,817,935 + -0.4 3,817,936 - -1.0 3,817,936 - +0.3 3,817,936 - +0.3 3,817,936 - -0.6 3,817,938 + +0.2 3,817,939 - -0.4 3,817,939 - -3.4 3,817,939 - -0.8 3,817,939 - -1.8 3,818,044 + -0.6 3,818,115 + RR42_RS37235 0.33 -1.0 3,818,116 - RR42_RS37235 0.34 +0.5 3,818,116 - RR42_RS37235 0.34 +0.4 3,818,224 + RR42_RS37235 0.77 +0.1 3,818,224 + RR42_RS37235 0.77 +1.0 3,818,224 + RR42_RS37235 0.77 -1.3 3,818,224 + RR42_RS37235 0.77 -0.1 3,818,225 - RR42_RS37235 0.77 -1.6 3,818,225 - RR42_RS37235 0.77 -0.3 3,818,225 - RR42_RS37235 0.77 +0.0 3,818,227 + RR42_RS37235 0.78 -0.8 3,818,228 - RR42_RS37235 0.78 +0.6 3,818,254 + RR42_RS37235 0.88 +0.2 3,818,254 + RR42_RS37235 0.88 -0.5 3,818,255 - RR42_RS37235 0.89 +0.6 3,818,255 - RR42_RS37235 0.89 +0.5 3,818,255 - RR42_RS37235 0.89 -2.9 3,818,265 - -0.7 3,818,336 + +0.4 3,818,419 - -3.2 3,818,482 - +0.9 3,818,482 - +1.5 3,818,538 - +2.0 3,818,545 + -0.9 3,818,610 - +0.1 3,818,723 + -0.7 3,818,763 - +1.0 3,818,776 + -0.5 3,818,776 + +1.0 3,818,776 + -1.3 3,818,776 + +0.7 3,818,777 - +0.5 3,818,777 - +0.5 3,818,777 - -0.2 3,818,779 + -1.4 3,818,926 + -0.6 3,818,926 + +0.5 3,818,926 + -1.2 3,818,926 + -2.1 3,818,926 + -0.9 3,818,927 - -1.7
Or see this region's nucleotide sequence