Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37165 and RR42_RS37170 are separated by 331 nucleotides RR42_RS37170 and RR42_RS37175 are separated by 67 nucleotides
RR42_RS37165: RR42_RS37165 - hypothetical protein, at 3,798,654 to 3,799,397
_RS37165
RR42_RS37170: RR42_RS37170 - MazF family transcriptional regulator, at 3,799,729 to 3,799,974
_RS37170
RR42_RS37175: RR42_RS37175 - hypothetical protein, at 3,800,042 to 3,800,974
_RS37175
Position (kb)
3799
3800 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3798.857 kb on + strand, within RR42_RS37165 at 3798.857 kb on + strand, within RR42_RS37165 at 3798.857 kb on + strand, within RR42_RS37165 at 3798.858 kb on - strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.861 kb on + strand, within RR42_RS37165 at 3798.862 kb on - strand, within RR42_RS37165 at 3798.862 kb on - strand, within RR42_RS37165 at 3798.862 kb on - strand, within RR42_RS37165 at 3798.862 kb on - strand, within RR42_RS37165 at 3798.862 kb on - strand, within RR42_RS37165 at 3798.862 kb on - strand, within RR42_RS37165 at 3798.862 kb on - strand, within RR42_RS37165 at 3798.866 kb on - strand, within RR42_RS37165 at 3798.978 kb on + strand, within RR42_RS37165 at 3799.025 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.164 kb on + strand, within RR42_RS37165 at 3799.165 kb on - strand, within RR42_RS37165 at 3799.165 kb on - strand, within RR42_RS37165 at 3799.165 kb on - strand, within RR42_RS37165 at 3799.165 kb on - strand, within RR42_RS37165 at 3799.225 kb on + strand, within RR42_RS37165 at 3799.225 kb on + strand, within RR42_RS37165 at 3799.225 kb on + strand, within RR42_RS37165 at 3799.225 kb on + strand, within RR42_RS37165 at 3799.225 kb on + strand, within RR42_RS37165 at 3799.225 kb on + strand, within RR42_RS37165 at 3799.225 kb on + strand, within RR42_RS37165 at 3799.226 kb on - strand, within RR42_RS37165 at 3799.226 kb on - strand, within RR42_RS37165 at 3799.239 kb on + strand, within RR42_RS37165 at 3799.239 kb on + strand, within RR42_RS37165 at 3799.239 kb on + strand, within RR42_RS37165 at 3799.240 kb on - strand, within RR42_RS37165 at 3799.240 kb on - strand, within RR42_RS37165 at 3799.285 kb on + strand, within RR42_RS37165 at 3799.286 kb on - strand, within RR42_RS37165 at 3799.286 kb on - strand, within RR42_RS37165 at 3799.328 kb on + strand at 3799.329 kb on - strand at 3799.424 kb on + strand at 3799.424 kb on + strand at 3799.424 kb on + strand at 3799.424 kb on + strand at 3799.425 kb on - strand at 3799.425 kb on - strand at 3799.500 kb on - strand at 3799.531 kb on + strand at 3799.532 kb on - strand at 3799.532 kb on - strand at 3799.573 kb on + strand at 3799.573 kb on + strand at 3799.573 kb on + strand at 3799.573 kb on + strand at 3799.573 kb on + strand at 3799.574 kb on - strand at 3799.574 kb on - strand at 3799.574 kb on - strand at 3799.656 kb on + strand at 3799.670 kb on + strand at 3799.671 kb on - strand at 3799.671 kb on - strand at 3799.671 kb on - strand at 3799.786 kb on + strand, within RR42_RS37170 at 3799.787 kb on - strand, within RR42_RS37170 at 3799.787 kb on - strand, within RR42_RS37170 at 3799.836 kb on - strand, within RR42_RS37170 at 3799.921 kb on - strand, within RR42_RS37170 at 3799.969 kb on + strand at 3799.970 kb on - strand at 3799.972 kb on + strand at 3799.973 kb on - strand at 3799.973 kb on - strand at 3799.978 kb on - strand at 3800.038 kb on - strand at 3800.043 kb on + strand at 3800.095 kb on + strand at 3800.095 kb on + strand at 3800.095 kb on + strand at 3800.096 kb on - strand at 3800.096 kb on - strand at 3800.097 kb on + strand at 3800.098 kb on - strand at 3800.098 kb on - strand at 3800.104 kb on + strand at 3800.104 kb on + strand at 3800.122 kb on - strand at 3800.136 kb on + strand, within RR42_RS37175 at 3800.192 kb on + strand, within RR42_RS37175 at 3800.193 kb on - strand, within RR42_RS37175 at 3800.272 kb on - strand, within RR42_RS37175 at 3800.287 kb on + strand, within RR42_RS37175 at 3800.287 kb on + strand, within RR42_RS37175 at 3800.321 kb on + strand, within RR42_RS37175 at 3800.321 kb on + strand, within RR42_RS37175 at 3800.321 kb on + strand, within RR42_RS37175 at 3800.321 kb on + strand, within RR42_RS37175 at 3800.322 kb on - strand, within RR42_RS37175 at 3800.322 kb on - strand, within RR42_RS37175 at 3800.322 kb on - strand, within RR42_RS37175 at 3800.322 kb on - strand, within RR42_RS37175 at 3800.348 kb on + strand, within RR42_RS37175 at 3800.348 kb on + strand, within RR42_RS37175 at 3800.349 kb on - strand, within RR42_RS37175 at 3800.349 kb on - strand, within RR42_RS37175 at 3800.349 kb on - strand, within RR42_RS37175 at 3800.349 kb on - strand, within RR42_RS37175 at 3800.400 kb on + strand, within RR42_RS37175 at 3800.401 kb on - strand, within RR42_RS37175 at 3800.401 kb on - strand, within RR42_RS37175 at 3800.401 kb on - strand, within RR42_RS37175 at 3800.455 kb on - strand, within RR42_RS37175 at 3800.455 kb on - strand, within RR42_RS37175 at 3800.473 kb on + strand, within RR42_RS37175 at 3800.473 kb on + strand, within RR42_RS37175 at 3800.479 kb on + strand, within RR42_RS37175 at 3800.486 kb on - strand, within RR42_RS37175 at 3800.654 kb on + strand, within RR42_RS37175 at 3800.740 kb on + strand, within RR42_RS37175 at 3800.758 kb on + strand, within RR42_RS37175 at 3800.758 kb on + strand, within RR42_RS37175 at 3800.758 kb on + strand, within RR42_RS37175 at 3800.758 kb on + strand, within RR42_RS37175 at 3800.758 kb on + strand, within RR42_RS37175 at 3800.867 kb on + strand, within RR42_RS37175 at 3800.952 kb on + strand at 3800.966 kb on - strand at 3800.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,798,857 + RR42_RS37165 0.27 +0.6 3,798,857 + RR42_RS37165 0.27 +0.9 3,798,857 + RR42_RS37165 0.27 +0.2 3,798,858 - RR42_RS37165 0.27 -2.1 3,798,861 + RR42_RS37165 0.28 +0.3 3,798,861 + RR42_RS37165 0.28 +0.4 3,798,861 + RR42_RS37165 0.28 -0.6 3,798,861 + RR42_RS37165 0.28 +0.1 3,798,861 + RR42_RS37165 0.28 +0.6 3,798,861 + RR42_RS37165 0.28 +0.6 3,798,861 + RR42_RS37165 0.28 -2.2 3,798,861 + RR42_RS37165 0.28 -1.3 3,798,861 + RR42_RS37165 0.28 +0.4 3,798,862 - RR42_RS37165 0.28 +1.0 3,798,862 - RR42_RS37165 0.28 -1.9 3,798,862 - RR42_RS37165 0.28 -0.0 3,798,862 - RR42_RS37165 0.28 +1.0 3,798,862 - RR42_RS37165 0.28 +2.0 3,798,862 - RR42_RS37165 0.28 -0.8 3,798,862 - RR42_RS37165 0.28 -0.2 3,798,866 - RR42_RS37165 0.28 -1.1 3,798,978 + RR42_RS37165 0.44 -2.2 3,799,025 + RR42_RS37165 0.50 -0.3 3,799,164 + RR42_RS37165 0.69 +0.2 3,799,164 + RR42_RS37165 0.69 -1.6 3,799,164 + RR42_RS37165 0.69 +0.3 3,799,164 + RR42_RS37165 0.69 -0.4 3,799,164 + RR42_RS37165 0.69 -0.5 3,799,164 + RR42_RS37165 0.69 +0.6 3,799,164 + RR42_RS37165 0.69 +0.7 3,799,164 + RR42_RS37165 0.69 -0.4 3,799,164 + RR42_RS37165 0.69 -0.2 3,799,165 - RR42_RS37165 0.69 -1.1 3,799,165 - RR42_RS37165 0.69 +1.5 3,799,165 - RR42_RS37165 0.69 +0.5 3,799,165 - RR42_RS37165 0.69 +0.7 3,799,225 + RR42_RS37165 0.77 -2.2 3,799,225 + RR42_RS37165 0.77 -0.4 3,799,225 + RR42_RS37165 0.77 +1.1 3,799,225 + RR42_RS37165 0.77 +0.9 3,799,225 + RR42_RS37165 0.77 +0.8 3,799,225 + RR42_RS37165 0.77 -1.9 3,799,225 + RR42_RS37165 0.77 -1.9 3,799,226 - RR42_RS37165 0.77 +0.4 3,799,226 - RR42_RS37165 0.77 -0.1 3,799,239 + RR42_RS37165 0.79 -0.2 3,799,239 + RR42_RS37165 0.79 +1.9 3,799,239 + RR42_RS37165 0.79 -0.2 3,799,240 - RR42_RS37165 0.79 +0.8 3,799,240 - RR42_RS37165 0.79 -1.9 3,799,285 + RR42_RS37165 0.85 +0.6 3,799,286 - RR42_RS37165 0.85 +0.1 3,799,286 - RR42_RS37165 0.85 +1.0 3,799,328 + -0.4 3,799,329 - +0.3 3,799,424 + +0.2 3,799,424 + -1.9 3,799,424 + -1.3 3,799,424 + +0.4 3,799,425 - -1.8 3,799,425 - -1.2 3,799,500 - -0.4 3,799,531 + +0.1 3,799,532 - -0.0 3,799,532 - -0.6 3,799,573 + -0.2 3,799,573 + -0.8 3,799,573 + +1.2 3,799,573 + -2.8 3,799,573 + +1.5 3,799,574 - -0.1 3,799,574 - +0.7 3,799,574 - +0.3 3,799,656 + +0.2 3,799,670 + -3.2 3,799,671 - +0.3 3,799,671 - +0.2 3,799,671 - -0.5 3,799,786 + RR42_RS37170 0.23 -0.7 3,799,787 - RR42_RS37170 0.24 +0.2 3,799,787 - RR42_RS37170 0.24 -1.1 3,799,836 - RR42_RS37170 0.43 -1.0 3,799,921 - RR42_RS37170 0.78 -2.4 3,799,969 + -2.5 3,799,970 - +0.7 3,799,972 + +1.3 3,799,973 - -0.8 3,799,973 - -0.3 3,799,978 - -0.9 3,800,038 - -0.7 3,800,043 + -3.0 3,800,095 + -0.6 3,800,095 + -2.2 3,800,095 + -0.2 3,800,096 - +1.2 3,800,096 - +1.0 3,800,097 + +0.5 3,800,098 - +0.6 3,800,098 - +1.8 3,800,104 + +0.3 3,800,104 + +0.9 3,800,122 - +0.3 3,800,136 + RR42_RS37175 0.10 -0.6 3,800,192 + RR42_RS37175 0.16 +0.7 3,800,193 - RR42_RS37175 0.16 +0.7 3,800,272 - RR42_RS37175 0.25 -0.5 3,800,287 + RR42_RS37175 0.26 -0.6 3,800,287 + RR42_RS37175 0.26 +1.1 3,800,321 + RR42_RS37175 0.30 +0.2 3,800,321 + RR42_RS37175 0.30 +0.0 3,800,321 + RR42_RS37175 0.30 -0.3 3,800,321 + RR42_RS37175 0.30 -0.3 3,800,322 - RR42_RS37175 0.30 -2.5 3,800,322 - RR42_RS37175 0.30 -1.2 3,800,322 - RR42_RS37175 0.30 -0.7 3,800,322 - RR42_RS37175 0.30 +0.1 3,800,348 + RR42_RS37175 0.33 +0.3 3,800,348 + RR42_RS37175 0.33 +0.3 3,800,349 - RR42_RS37175 0.33 +0.2 3,800,349 - RR42_RS37175 0.33 +0.4 3,800,349 - RR42_RS37175 0.33 -0.4 3,800,349 - RR42_RS37175 0.33 -1.3 3,800,400 + RR42_RS37175 0.38 -1.1 3,800,401 - RR42_RS37175 0.38 -2.4 3,800,401 - RR42_RS37175 0.38 +0.6 3,800,401 - RR42_RS37175 0.38 -0.9 3,800,455 - RR42_RS37175 0.44 -1.8 3,800,455 - RR42_RS37175 0.44 +0.6 3,800,473 + RR42_RS37175 0.46 +1.0 3,800,473 + RR42_RS37175 0.46 -3.3 3,800,479 + RR42_RS37175 0.47 -2.9 3,800,486 - RR42_RS37175 0.48 +0.1 3,800,654 + RR42_RS37175 0.66 +0.3 3,800,740 + RR42_RS37175 0.75 +1.2 3,800,758 + RR42_RS37175 0.77 +0.8 3,800,758 + RR42_RS37175 0.77 +0.6 3,800,758 + RR42_RS37175 0.77 +0.1 3,800,758 + RR42_RS37175 0.77 +1.9 3,800,758 + RR42_RS37175 0.77 -0.3 3,800,867 + RR42_RS37175 0.88 +0.2 3,800,952 + -0.7 3,800,966 - -3.4 3,800,966 - +0.9
Or see this region's nucleotide sequence