Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS37085 and RR42_RS37090 are separated by 63 nucleotides RR42_RS37090 and RR42_RS37095 overlap by 4 nucleotides
RR42_RS37085: RR42_RS37085 - ATP-dependent DNA ligase, at 3,783,682 to 3,786,183
_RS37085
RR42_RS37090: RR42_RS37090 - DNA repair protein, at 3,786,247 to 3,787,161
_RS37090
RR42_RS37095: RR42_RS37095 - hypothetical protein, at 3,787,158 to 3,787,994
_RS37095
Position (kb)
3783
3784
3785
3786
3787 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3782.689 kb on + strand at 3782.690 kb on - strand at 3782.705 kb on - strand at 3782.715 kb on + strand at 3782.715 kb on + strand at 3782.725 kb on + strand at 3782.849 kb on + strand at 3782.850 kb on - strand at 3782.850 kb on - strand at 3782.892 kb on - strand at 3782.892 kb on - strand at 3782.893 kb on + strand at 3782.894 kb on - strand at 3782.984 kb on + strand at 3783.114 kb on - strand at 3783.153 kb on + strand at 3783.154 kb on - strand at 3783.330 kb on - strand at 3783.330 kb on - strand at 3783.330 kb on - strand at 3783.330 kb on - strand at 3783.375 kb on + strand at 3783.375 kb on + strand at 3783.456 kb on - strand at 3783.478 kb on + strand at 3783.478 kb on + strand at 3783.479 kb on - strand at 3783.479 kb on - strand at 3783.516 kb on + strand at 3783.588 kb on - strand at 3783.608 kb on - strand at 3783.618 kb on + strand at 3783.630 kb on + strand at 3783.630 kb on + strand at 3783.630 kb on + strand at 3783.630 kb on + strand at 3783.666 kb on - strand at 3783.671 kb on + strand at 3783.671 kb on + strand at 3783.671 kb on + strand at 3783.672 kb on - strand at 3783.696 kb on + strand at 3783.696 kb on + strand at 3783.718 kb on + strand at 3783.718 kb on + strand at 3783.718 kb on + strand at 3783.771 kb on + strand at 3783.835 kb on + strand at 3783.835 kb on + strand at 3783.866 kb on + strand at 3783.867 kb on - strand at 3783.952 kb on + strand, within RR42_RS37085 at 3783.989 kb on - strand, within RR42_RS37085 at 3783.989 kb on - strand, within RR42_RS37085 at 3784.163 kb on + strand, within RR42_RS37085 at 3784.163 kb on + strand, within RR42_RS37085 at 3784.164 kb on - strand, within RR42_RS37085 at 3784.164 kb on - strand, within RR42_RS37085 at 3784.164 kb on - strand, within RR42_RS37085 at 3784.164 kb on - strand, within RR42_RS37085 at 3784.164 kb on - strand, within RR42_RS37085 at 3784.370 kb on + strand, within RR42_RS37085 at 3784.379 kb on + strand, within RR42_RS37085 at 3784.380 kb on - strand, within RR42_RS37085 at 3784.380 kb on - strand, within RR42_RS37085 at 3784.654 kb on + strand, within RR42_RS37085 at 3784.655 kb on - strand, within RR42_RS37085 at 3784.830 kb on - strand, within RR42_RS37085 at 3784.864 kb on + strand, within RR42_RS37085 at 3784.864 kb on + strand, within RR42_RS37085 at 3784.864 kb on + strand, within RR42_RS37085 at 3784.865 kb on - strand, within RR42_RS37085 at 3784.865 kb on - strand, within RR42_RS37085 at 3784.865 kb on - strand, within RR42_RS37085 at 3784.893 kb on - strand, within RR42_RS37085 at 3784.976 kb on + strand, within RR42_RS37085 at 3784.977 kb on - strand, within RR42_RS37085 at 3784.977 kb on - strand, within RR42_RS37085 at 3784.977 kb on - strand, within RR42_RS37085 at 3784.977 kb on - strand, within RR42_RS37085 at 3785.012 kb on - strand, within RR42_RS37085 at 3785.381 kb on + strand, within RR42_RS37085 at 3785.381 kb on + strand, within RR42_RS37085 at 3785.381 kb on + strand, within RR42_RS37085 at 3785.382 kb on - strand, within RR42_RS37085 at 3785.382 kb on - strand, within RR42_RS37085 at 3785.389 kb on + strand, within RR42_RS37085 at 3785.392 kb on + strand, within RR42_RS37085 at 3785.393 kb on - strand, within RR42_RS37085 at 3785.393 kb on - strand, within RR42_RS37085 at 3785.417 kb on + strand at 3785.418 kb on - strand, within RR42_RS37085 at 3785.418 kb on - strand, within RR42_RS37085 at 3785.591 kb on + strand, within RR42_RS37085 at 3785.625 kb on + strand, within RR42_RS37085 at 3785.710 kb on + strand, within RR42_RS37085 at 3785.710 kb on + strand, within RR42_RS37085 at 3785.711 kb on - strand, within RR42_RS37085 at 3785.711 kb on - strand, within RR42_RS37085 at 3785.744 kb on + strand, within RR42_RS37085 at 3785.833 kb on + strand, within RR42_RS37085 at 3785.834 kb on - strand, within RR42_RS37085 at 3785.834 kb on - strand, within RR42_RS37085 at 3785.867 kb on + strand, within RR42_RS37085 at 3785.868 kb on - strand, within RR42_RS37085 at 3785.868 kb on - strand, within RR42_RS37085 at 3785.950 kb on + strand at 3786.048 kb on + strand at 3786.048 kb on + strand at 3786.048 kb on + strand at 3786.048 kb on + strand at 3786.048 kb on + strand at 3786.048 kb on + strand at 3786.049 kb on - strand at 3786.049 kb on - strand at 3786.049 kb on - strand at 3786.049 kb on - strand at 3786.049 kb on - strand at 3786.102 kb on + strand at 3786.102 kb on + strand at 3786.102 kb on + strand at 3786.102 kb on + strand at 3786.102 kb on + strand at 3786.102 kb on + strand at 3786.103 kb on - strand at 3786.134 kb on - strand at 3786.134 kb on - strand at 3786.134 kb on - strand at 3786.168 kb on + strand at 3786.168 kb on + strand at 3786.169 kb on - strand at 3786.169 kb on - strand at 3786.243 kb on + strand at 3786.244 kb on - strand at 3786.244 kb on - strand at 3786.244 kb on - strand at 3786.246 kb on + strand at 3786.246 kb on + strand at 3786.246 kb on + strand at 3786.246 kb on + strand at 3786.247 kb on - strand at 3786.247 kb on - strand at 3786.311 kb on + strand at 3786.311 kb on + strand at 3786.312 kb on - strand at 3786.312 kb on - strand at 3786.312 kb on - strand at 3786.312 kb on - strand at 3786.314 kb on - strand at 3786.353 kb on - strand, within RR42_RS37090 at 3786.353 kb on - strand, within RR42_RS37090 at 3786.388 kb on + strand, within RR42_RS37090 at 3786.443 kb on - strand, within RR42_RS37090 at 3786.443 kb on - strand, within RR42_RS37090 at 3786.463 kb on + strand, within RR42_RS37090 at 3786.565 kb on + strand, within RR42_RS37090 at 3786.565 kb on + strand, within RR42_RS37090 at 3786.565 kb on + strand, within RR42_RS37090 at 3786.566 kb on - strand, within RR42_RS37090 at 3786.580 kb on + strand, within RR42_RS37090 at 3786.616 kb on + strand, within RR42_RS37090 at 3786.617 kb on - strand, within RR42_RS37090 at 3786.693 kb on + strand, within RR42_RS37090 at 3786.693 kb on + strand, within RR42_RS37090 at 3786.694 kb on - strand, within RR42_RS37090 at 3786.813 kb on - strand, within RR42_RS37090 at 3786.852 kb on + strand, within RR42_RS37090 at 3786.852 kb on + strand, within RR42_RS37090 at 3786.853 kb on - strand, within RR42_RS37090 at 3786.853 kb on - strand, within RR42_RS37090 at 3786.857 kb on - strand, within RR42_RS37090 at 3786.857 kb on - strand, within RR42_RS37090 at 3786.857 kb on - strand, within RR42_RS37090 at 3786.978 kb on + strand, within RR42_RS37090 at 3786.979 kb on - strand, within RR42_RS37090 at 3786.979 kb on - strand, within RR42_RS37090 at 3786.979 kb on - strand, within RR42_RS37090 at 3786.987 kb on + strand, within RR42_RS37090 at 3786.987 kb on + strand, within RR42_RS37090 at 3786.987 kb on + strand, within RR42_RS37090
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,782,689 + +1.7 3,782,690 - -0.4 3,782,705 - -0.2 3,782,715 + -1.6 3,782,715 + -1.2 3,782,725 + +0.8 3,782,849 + +1.2 3,782,850 - -0.8 3,782,850 - +1.4 3,782,892 - -0.6 3,782,892 - -1.0 3,782,893 + -1.9 3,782,894 - -1.5 3,782,984 + -0.2 3,783,114 - +1.9 3,783,153 + -0.4 3,783,154 - +0.5 3,783,330 - -1.8 3,783,330 - -2.3 3,783,330 - -1.8 3,783,330 - +0.3 3,783,375 + -2.3 3,783,375 + -0.4 3,783,456 - -3.5 3,783,478 + -1.1 3,783,478 + -2.9 3,783,479 - -0.3 3,783,479 - -0.7 3,783,516 + -3.7 3,783,588 - +0.2 3,783,608 - -1.1 3,783,618 + -0.4 3,783,630 + +2.0 3,783,630 + +0.9 3,783,630 + +0.7 3,783,630 + -2.4 3,783,666 - +0.4 3,783,671 + -0.5 3,783,671 + +1.3 3,783,671 + +0.1 3,783,672 - +1.3 3,783,696 + -0.6 3,783,696 + -1.1 3,783,718 + +0.7 3,783,718 + +0.3 3,783,718 + +0.1 3,783,771 + +0.2 3,783,835 + -0.4 3,783,835 + +1.3 3,783,866 + +2.8 3,783,867 - +0.5 3,783,952 + RR42_RS37085 0.11 -0.0 3,783,989 - RR42_RS37085 0.12 -0.2 3,783,989 - RR42_RS37085 0.12 -1.3 3,784,163 + RR42_RS37085 0.19 +2.6 3,784,163 + RR42_RS37085 0.19 +0.7 3,784,164 - RR42_RS37085 0.19 +2.1 3,784,164 - RR42_RS37085 0.19 +1.0 3,784,164 - RR42_RS37085 0.19 -0.3 3,784,164 - RR42_RS37085 0.19 +0.9 3,784,164 - RR42_RS37085 0.19 +3.0 3,784,370 + RR42_RS37085 0.27 +1.6 3,784,379 + RR42_RS37085 0.28 +1.5 3,784,380 - RR42_RS37085 0.28 -0.4 3,784,380 - RR42_RS37085 0.28 -1.3 3,784,654 + RR42_RS37085 0.39 +0.4 3,784,655 - RR42_RS37085 0.39 +2.7 3,784,830 - RR42_RS37085 0.46 -0.3 3,784,864 + RR42_RS37085 0.47 -0.9 3,784,864 + RR42_RS37085 0.47 +1.6 3,784,864 + RR42_RS37085 0.47 -2.6 3,784,865 - RR42_RS37085 0.47 -1.0 3,784,865 - RR42_RS37085 0.47 +0.7 3,784,865 - RR42_RS37085 0.47 -0.3 3,784,893 - RR42_RS37085 0.48 +0.8 3,784,976 + RR42_RS37085 0.52 +0.0 3,784,977 - RR42_RS37085 0.52 -1.9 3,784,977 - RR42_RS37085 0.52 -0.3 3,784,977 - RR42_RS37085 0.52 -0.2 3,784,977 - RR42_RS37085 0.52 +0.6 3,785,012 - RR42_RS37085 0.53 -0.1 3,785,381 + RR42_RS37085 0.68 +1.9 3,785,381 + RR42_RS37085 0.68 -2.0 3,785,381 + RR42_RS37085 0.68 +0.5 3,785,382 - RR42_RS37085 0.68 +3.2 3,785,382 - RR42_RS37085 0.68 -0.2 3,785,389 + RR42_RS37085 0.68 +0.0 3,785,392 + RR42_RS37085 0.68 -0.6 3,785,393 - RR42_RS37085 0.68 -0.8 3,785,393 - RR42_RS37085 0.68 -1.8 3,785,417 + +1.2 3,785,418 - RR42_RS37085 0.69 +1.2 3,785,418 - RR42_RS37085 0.69 -1.3 3,785,591 + RR42_RS37085 0.76 +0.5 3,785,625 + RR42_RS37085 0.78 +0.9 3,785,710 + RR42_RS37085 0.81 +1.4 3,785,710 + RR42_RS37085 0.81 +0.8 3,785,711 - RR42_RS37085 0.81 +0.3 3,785,711 - RR42_RS37085 0.81 -2.4 3,785,744 + RR42_RS37085 0.82 +1.2 3,785,833 + RR42_RS37085 0.86 -1.9 3,785,834 - RR42_RS37085 0.86 -0.1 3,785,834 - RR42_RS37085 0.86 -0.6 3,785,867 + RR42_RS37085 0.87 -1.4 3,785,868 - RR42_RS37085 0.87 -3.0 3,785,868 - RR42_RS37085 0.87 -2.2 3,785,950 + +0.2 3,786,048 + -2.3 3,786,048 + +0.5 3,786,048 + -0.2 3,786,048 + -0.5 3,786,048 + +1.8 3,786,048 + +0.1 3,786,049 - +0.4 3,786,049 - +1.6 3,786,049 - +0.4 3,786,049 - +0.7 3,786,049 - +1.9 3,786,102 + -0.7 3,786,102 + -0.6 3,786,102 + -1.9 3,786,102 + +0.0 3,786,102 + +0.3 3,786,102 + -2.1 3,786,103 - -0.5 3,786,134 - -2.4 3,786,134 - -0.8 3,786,134 - -1.7 3,786,168 + +1.2 3,786,168 + +1.9 3,786,169 - +0.6 3,786,169 - +1.1 3,786,243 + -3.2 3,786,244 - -0.1 3,786,244 - +0.1 3,786,244 - +1.1 3,786,246 + -0.8 3,786,246 + +0.2 3,786,246 + +0.7 3,786,246 + -0.2 3,786,247 - +0.2 3,786,247 - -1.6 3,786,311 + -1.9 3,786,311 + +1.6 3,786,312 - +1.2 3,786,312 - -0.0 3,786,312 - -2.5 3,786,312 - -0.8 3,786,314 - -2.9 3,786,353 - RR42_RS37090 0.12 -0.4 3,786,353 - RR42_RS37090 0.12 -2.5 3,786,388 + RR42_RS37090 0.15 -0.5 3,786,443 - RR42_RS37090 0.21 -0.4 3,786,443 - RR42_RS37090 0.21 -0.4 3,786,463 + RR42_RS37090 0.24 -2.6 3,786,565 + RR42_RS37090 0.35 -0.1 3,786,565 + RR42_RS37090 0.35 +1.0 3,786,565 + RR42_RS37090 0.35 +0.9 3,786,566 - RR42_RS37090 0.35 +0.8 3,786,580 + RR42_RS37090 0.36 +0.7 3,786,616 + RR42_RS37090 0.40 +0.2 3,786,617 - RR42_RS37090 0.40 -1.8 3,786,693 + RR42_RS37090 0.49 +2.3 3,786,693 + RR42_RS37090 0.49 +1.8 3,786,694 - RR42_RS37090 0.49 -2.7 3,786,813 - RR42_RS37090 0.62 -0.2 3,786,852 + RR42_RS37090 0.66 +0.9 3,786,852 + RR42_RS37090 0.66 -0.6 3,786,853 - RR42_RS37090 0.66 +0.5 3,786,853 - RR42_RS37090 0.66 -0.1 3,786,857 - RR42_RS37090 0.67 +2.2 3,786,857 - RR42_RS37090 0.67 +1.1 3,786,857 - RR42_RS37090 0.67 -0.7 3,786,978 + RR42_RS37090 0.80 +0.2 3,786,979 - RR42_RS37090 0.80 +0.0 3,786,979 - RR42_RS37090 0.80 +1.4 3,786,979 - RR42_RS37090 0.80 -0.9 3,786,987 + RR42_RS37090 0.81 -1.1 3,786,987 + RR42_RS37090 0.81 +0.6 3,786,987 + RR42_RS37090 0.81 -0.8
Or see this region's nucleotide sequence