Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36630 and RR42_RS36635 are separated by 125 nucleotides RR42_RS36635 and RR42_RS36640 are separated by 80 nucleotides
RR42_RS36630: RR42_RS36630 - ABC transporter substrate-binding protein, at 3,672,127 to 3,673,089
_RS36630
RR42_RS36635: RR42_RS36635 - long-chain fatty acid--CoA ligase, at 3,673,215 to 3,674,759
_RS36635
RR42_RS36640: RR42_RS36640 - ABC transporter substrate-binding protein, at 3,674,840 to 3,675,820
_RS36640
Position (kb)
3673
3674
3675 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3672.220 kb on - strand at 3672.345 kb on - strand, within RR42_RS36630 at 3672.363 kb on - strand, within RR42_RS36630 at 3672.548 kb on + strand, within RR42_RS36630 at 3672.549 kb on - strand, within RR42_RS36630 at 3672.729 kb on - strand, within RR42_RS36630 at 3672.749 kb on + strand, within RR42_RS36630 at 3672.749 kb on + strand, within RR42_RS36630 at 3672.830 kb on + strand, within RR42_RS36630 at 3672.941 kb on + strand, within RR42_RS36630 at 3672.951 kb on - strand, within RR42_RS36630 at 3672.989 kb on + strand, within RR42_RS36630 at 3673.327 kb on + strand at 3673.351 kb on + strand at 3673.352 kb on - strand at 3673.401 kb on - strand, within RR42_RS36635 at 3673.553 kb on + strand, within RR42_RS36635 at 3673.759 kb on + strand, within RR42_RS36635 at 3673.759 kb on + strand, within RR42_RS36635 at 3673.819 kb on + strand, within RR42_RS36635 at 3673.912 kb on + strand, within RR42_RS36635 at 3673.913 kb on - strand, within RR42_RS36635 at 3673.913 kb on - strand, within RR42_RS36635 at 3673.913 kb on - strand, within RR42_RS36635 at 3673.913 kb on - strand, within RR42_RS36635 at 3673.913 kb on - strand, within RR42_RS36635 at 3674.096 kb on - strand, within RR42_RS36635 at 3674.269 kb on + strand, within RR42_RS36635 at 3674.270 kb on - strand, within RR42_RS36635 at 3674.270 kb on - strand, within RR42_RS36635 at 3674.270 kb on - strand, within RR42_RS36635 at 3674.660 kb on + strand at 3674.660 kb on + strand at 3674.661 kb on - strand at 3674.661 kb on - strand at 3674.661 kb on - strand at 3674.775 kb on + strand at 3674.776 kb on - strand at 3674.813 kb on + strand at 3674.813 kb on + strand at 3674.893 kb on - strand at 3674.905 kb on + strand at 3674.907 kb on + strand at 3674.907 kb on + strand at 3674.908 kb on - strand at 3674.908 kb on - strand at 3674.908 kb on - strand at 3674.908 kb on - strand at 3675.181 kb on - strand, within RR42_RS36640 at 3675.264 kb on + strand, within RR42_RS36640 at 3675.265 kb on - strand, within RR42_RS36640 at 3675.351 kb on + strand, within RR42_RS36640 at 3675.352 kb on - strand, within RR42_RS36640 at 3675.447 kb on + strand, within RR42_RS36640 at 3675.448 kb on - strand, within RR42_RS36640 at 3675.553 kb on + strand, within RR42_RS36640 at 3675.554 kb on - strand, within RR42_RS36640 at 3675.554 kb on - strand, within RR42_RS36640 at 3675.554 kb on - strand, within RR42_RS36640 at 3675.554 kb on - strand, within RR42_RS36640 at 3675.554 kb on - strand, within RR42_RS36640 at 3675.555 kb on + strand, within RR42_RS36640
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,672,220 - -0.4 3,672,345 - RR42_RS36630 0.23 +0.2 3,672,363 - RR42_RS36630 0.25 +0.1 3,672,548 + RR42_RS36630 0.44 +1.2 3,672,549 - RR42_RS36630 0.44 +0.5 3,672,729 - RR42_RS36630 0.63 +0.1 3,672,749 + RR42_RS36630 0.65 -1.0 3,672,749 + RR42_RS36630 0.65 -0.3 3,672,830 + RR42_RS36630 0.73 -1.8 3,672,941 + RR42_RS36630 0.85 +0.8 3,672,951 - RR42_RS36630 0.86 -0.4 3,672,989 + RR42_RS36630 0.90 +1.9 3,673,327 + -0.2 3,673,351 + +0.1 3,673,352 - -0.1 3,673,401 - RR42_RS36635 0.12 +1.2 3,673,553 + RR42_RS36635 0.22 +0.1 3,673,759 + RR42_RS36635 0.35 +3.2 3,673,759 + RR42_RS36635 0.35 -1.7 3,673,819 + RR42_RS36635 0.39 -0.2 3,673,912 + RR42_RS36635 0.45 -0.7 3,673,913 - RR42_RS36635 0.45 -2.4 3,673,913 - RR42_RS36635 0.45 -0.9 3,673,913 - RR42_RS36635 0.45 +0.9 3,673,913 - RR42_RS36635 0.45 +0.6 3,673,913 - RR42_RS36635 0.45 -0.5 3,674,096 - RR42_RS36635 0.57 +1.0 3,674,269 + RR42_RS36635 0.68 +1.0 3,674,270 - RR42_RS36635 0.68 -1.0 3,674,270 - RR42_RS36635 0.68 -0.9 3,674,270 - RR42_RS36635 0.68 -1.6 3,674,660 + -0.7 3,674,660 + -2.2 3,674,661 - -0.7 3,674,661 - +1.1 3,674,661 - -0.6 3,674,775 + -0.8 3,674,776 - +0.3 3,674,813 + -2.1 3,674,813 + -0.8 3,674,893 - +0.4 3,674,905 + -0.4 3,674,907 + +0.2 3,674,907 + -1.2 3,674,908 - -0.5 3,674,908 - -0.5 3,674,908 - +0.0 3,674,908 - +0.4 3,675,181 - RR42_RS36640 0.35 -1.1 3,675,264 + RR42_RS36640 0.43 -2.2 3,675,265 - RR42_RS36640 0.43 +0.9 3,675,351 + RR42_RS36640 0.52 -0.8 3,675,352 - RR42_RS36640 0.52 +1.3 3,675,447 + RR42_RS36640 0.62 +0.1 3,675,448 - RR42_RS36640 0.62 -0.0 3,675,553 + RR42_RS36640 0.73 +0.8 3,675,554 - RR42_RS36640 0.73 +1.8 3,675,554 - RR42_RS36640 0.73 -0.3 3,675,554 - RR42_RS36640 0.73 +1.2 3,675,554 - RR42_RS36640 0.73 -2.4 3,675,554 - RR42_RS36640 0.73 +0.6 3,675,555 + RR42_RS36640 0.73 +0.6
Or see this region's nucleotide sequence