Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36485 and RR42_RS36490 are separated by 432 nucleotides RR42_RS36490 and RR42_RS36495 are separated by 91 nucleotides RR42_RS36495 and RR42_RS36500 are separated by 60 nucleotides
RR42_RS36485: RR42_RS36485 - serine/threonine protein kinase, at 3,640,868 to 3,642,790
_RS36485
RR42_RS36490: RR42_RS36490 - acyl-CoA dehydrogenase, at 3,643,223 to 3,644,410
_RS36490
RR42_RS36495: RR42_RS36495 - enoyl-CoA hydratase, at 3,644,502 to 3,645,281
_RS36495
RR42_RS36500: RR42_RS36500 - ABC transporter substrate-binding protein, at 3,645,342 to 3,646,328
_RS36500
Position (kb)
3643
3644
3645 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3642.310 kb on - strand, within RR42_RS36485 at 3642.472 kb on - strand, within RR42_RS36485 at 3642.555 kb on + strand, within RR42_RS36485 at 3642.688 kb on - strand at 3642.721 kb on - strand at 3642.735 kb on + strand at 3642.735 kb on + strand at 3642.736 kb on - strand at 3642.736 kb on - strand at 3642.771 kb on + strand at 3642.771 kb on + strand at 3642.790 kb on + strand at 3642.791 kb on - strand at 3642.791 kb on - strand at 3642.828 kb on - strand at 3642.828 kb on - strand at 3642.828 kb on - strand at 3642.833 kb on + strand at 3642.834 kb on - strand at 3642.836 kb on + strand at 3642.836 kb on + strand at 3642.837 kb on - strand at 3642.837 kb on - strand at 3642.843 kb on + strand at 3642.844 kb on - strand at 3642.844 kb on - strand at 3642.844 kb on - strand at 3643.010 kb on - strand at 3643.068 kb on + strand at 3643.068 kb on + strand at 3643.068 kb on + strand at 3643.068 kb on + strand at 3643.069 kb on - strand at 3643.163 kb on + strand at 3643.327 kb on + strand at 3643.327 kb on + strand at 3643.328 kb on - strand at 3643.328 kb on - strand at 3643.329 kb on + strand at 3643.526 kb on - strand, within RR42_RS36490 at 3643.881 kb on + strand, within RR42_RS36490 at 3643.881 kb on + strand, within RR42_RS36490 at 3643.920 kb on + strand, within RR42_RS36490 at 3643.921 kb on - strand, within RR42_RS36490 at 3643.921 kb on - strand, within RR42_RS36490 at 3643.921 kb on - strand, within RR42_RS36490 at 3644.073 kb on + strand, within RR42_RS36490 at 3644.073 kb on + strand, within RR42_RS36490 at 3644.085 kb on + strand, within RR42_RS36490 at 3644.085 kb on + strand, within RR42_RS36490 at 3644.265 kb on + strand, within RR42_RS36490 at 3644.324 kb on + strand at 3644.324 kb on + strand at 3644.325 kb on - strand at 3644.485 kb on + strand at 3644.485 kb on + strand at 3644.485 kb on + strand at 3644.486 kb on - strand at 3644.486 kb on - strand at 3644.486 kb on - strand at 3644.848 kb on + strand, within RR42_RS36495 at 3644.848 kb on + strand, within RR42_RS36495 at 3644.848 kb on + strand, within RR42_RS36495 at 3644.849 kb on - strand, within RR42_RS36495 at 3644.849 kb on - strand, within RR42_RS36495 at 3644.849 kb on - strand, within RR42_RS36495 at 3645.265 kb on + strand at 3645.266 kb on - strand at 3645.312 kb on - strand at 3645.329 kb on + strand at 3645.329 kb on + strand at 3645.330 kb on - strand at 3645.330 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,642,310 - RR42_RS36485 0.75 -1.2 3,642,472 - RR42_RS36485 0.83 +0.4 3,642,555 + RR42_RS36485 0.88 -0.0 3,642,688 - +0.9 3,642,721 - +0.0 3,642,735 + -1.1 3,642,735 + +1.7 3,642,736 - -0.6 3,642,736 - +0.9 3,642,771 + -0.5 3,642,771 + +0.9 3,642,790 + +0.4 3,642,791 - +0.2 3,642,791 - +0.1 3,642,828 - -0.3 3,642,828 - +0.1 3,642,828 - +1.4 3,642,833 + -0.5 3,642,834 - -1.6 3,642,836 + -1.8 3,642,836 + -0.3 3,642,837 - -0.5 3,642,837 - +0.3 3,642,843 + -1.5 3,642,844 - +0.7 3,642,844 - +0.9 3,642,844 - -0.2 3,643,010 - -1.6 3,643,068 + -0.4 3,643,068 + -0.9 3,643,068 + +0.3 3,643,068 + -1.6 3,643,069 - +1.7 3,643,163 + +0.4 3,643,327 + -0.7 3,643,327 + -0.3 3,643,328 - -2.8 3,643,328 - -0.1 3,643,329 + -1.6 3,643,526 - RR42_RS36490 0.26 +0.2 3,643,881 + RR42_RS36490 0.55 -0.0 3,643,881 + RR42_RS36490 0.55 -0.4 3,643,920 + RR42_RS36490 0.59 -0.3 3,643,921 - RR42_RS36490 0.59 -0.6 3,643,921 - RR42_RS36490 0.59 -1.1 3,643,921 - RR42_RS36490 0.59 -1.2 3,644,073 + RR42_RS36490 0.72 -0.6 3,644,073 + RR42_RS36490 0.72 -0.4 3,644,085 + RR42_RS36490 0.73 -2.8 3,644,085 + RR42_RS36490 0.73 -4.0 3,644,265 + RR42_RS36490 0.88 -0.7 3,644,324 + +2.1 3,644,324 + +0.7 3,644,325 - +0.1 3,644,485 + -0.3 3,644,485 + -0.6 3,644,485 + +1.5 3,644,486 - -2.4 3,644,486 - -2.4 3,644,486 - -1.3 3,644,848 + RR42_RS36495 0.44 +0.4 3,644,848 + RR42_RS36495 0.44 -2.0 3,644,848 + RR42_RS36495 0.44 -0.6 3,644,849 - RR42_RS36495 0.44 +0.2 3,644,849 - RR42_RS36495 0.44 +0.6 3,644,849 - RR42_RS36495 0.44 +0.4 3,645,265 + +0.1 3,645,266 - +0.8 3,645,312 - -0.1 3,645,329 + -0.4 3,645,329 + -0.0 3,645,330 - -1.1 3,645,330 - -1.6
Or see this region's nucleotide sequence