Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36440 and RR42_RS36445 are separated by 156 nucleotides RR42_RS36445 and RR42_RS36450 are separated by 69 nucleotides RR42_RS36450 and RR42_RS36455 are separated by 19 nucleotides
RR42_RS36440: RR42_RS36440 - LysR family transcriptional regulator, at 3,630,667 to 3,631,557
_RS36440
RR42_RS36445: RR42_RS36445 - acetyl-CoA acetyltransferase, at 3,631,714 to 3,632,922
_RS36445
RR42_RS36450: RR42_RS36450 - acyl dehydratase, at 3,632,992 to 3,633,453
_RS36450
RR42_RS36455: RR42_RS36455 - acyl-CoA dehydrogenase, at 3,633,473 to 3,634,633
_RS36455
Position (kb)
3631
3632
3633 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3630.723 kb on + strand at 3630.807 kb on + strand, within RR42_RS36440 at 3630.807 kb on + strand, within RR42_RS36440 at 3630.807 kb on + strand, within RR42_RS36440 at 3630.807 kb on + strand, within RR42_RS36440 at 3630.809 kb on + strand, within RR42_RS36440 at 3630.810 kb on - strand, within RR42_RS36440 at 3630.810 kb on - strand, within RR42_RS36440 at 3630.810 kb on - strand, within RR42_RS36440 at 3630.810 kb on - strand, within RR42_RS36440 at 3631.091 kb on + strand, within RR42_RS36440 at 3631.092 kb on - strand, within RR42_RS36440 at 3631.250 kb on + strand, within RR42_RS36440 at 3631.251 kb on - strand, within RR42_RS36440 at 3631.251 kb on - strand, within RR42_RS36440 at 3631.407 kb on + strand, within RR42_RS36440 at 3631.407 kb on + strand, within RR42_RS36440 at 3631.408 kb on - strand, within RR42_RS36440 at 3631.635 kb on - strand at 3631.635 kb on - strand at 3631.772 kb on - strand at 3631.772 kb on - strand at 3632.123 kb on - strand, within RR42_RS36445 at 3632.191 kb on + strand, within RR42_RS36445 at 3632.192 kb on - strand, within RR42_RS36445 at 3632.192 kb on - strand, within RR42_RS36445 at 3632.350 kb on + strand, within RR42_RS36445 at 3632.350 kb on + strand, within RR42_RS36445 at 3632.422 kb on + strand, within RR42_RS36445 at 3632.893 kb on + strand at 3632.894 kb on - strand at 3632.913 kb on + strand at 3632.914 kb on - strand at 3632.914 kb on - strand at 3632.914 kb on - strand at 3632.944 kb on - strand at 3632.948 kb on + strand at 3632.948 kb on + strand at 3632.948 kb on + strand at 3632.949 kb on - strand at 3632.949 kb on - strand at 3633.031 kb on + strand at 3633.031 kb on + strand at 3633.032 kb on - strand at 3633.203 kb on - strand, within RR42_RS36450 at 3633.386 kb on - strand, within RR42_RS36450 at 3633.421 kb on + strand at 3633.421 kb on + strand at 3633.422 kb on - strand at 3633.827 kb on + strand, within RR42_RS36455 at 3633.827 kb on + strand, within RR42_RS36455 at 3633.828 kb on - strand, within RR42_RS36455 at 3633.828 kb on - strand, within RR42_RS36455 at 3633.828 kb on - strand, within RR42_RS36455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,630,723 + +0.7 3,630,807 + RR42_RS36440 0.16 +0.4 3,630,807 + RR42_RS36440 0.16 -0.8 3,630,807 + RR42_RS36440 0.16 +1.6 3,630,807 + RR42_RS36440 0.16 +0.5 3,630,809 + RR42_RS36440 0.16 -0.9 3,630,810 - RR42_RS36440 0.16 -0.6 3,630,810 - RR42_RS36440 0.16 +1.8 3,630,810 - RR42_RS36440 0.16 -0.1 3,630,810 - RR42_RS36440 0.16 -0.0 3,631,091 + RR42_RS36440 0.48 -0.1 3,631,092 - RR42_RS36440 0.48 +0.3 3,631,250 + RR42_RS36440 0.65 +0.2 3,631,251 - RR42_RS36440 0.66 +1.0 3,631,251 - RR42_RS36440 0.66 -0.5 3,631,407 + RR42_RS36440 0.83 -0.2 3,631,407 + RR42_RS36440 0.83 +0.4 3,631,408 - RR42_RS36440 0.83 +1.2 3,631,635 - -0.2 3,631,635 - -0.7 3,631,772 - -1.4 3,631,772 - -1.0 3,632,123 - RR42_RS36445 0.34 -0.2 3,632,191 + RR42_RS36445 0.39 +2.2 3,632,192 - RR42_RS36445 0.40 +1.8 3,632,192 - RR42_RS36445 0.40 -0.1 3,632,350 + RR42_RS36445 0.53 -2.3 3,632,350 + RR42_RS36445 0.53 -1.5 3,632,422 + RR42_RS36445 0.59 -2.0 3,632,893 + +1.5 3,632,894 - +0.0 3,632,913 + -0.4 3,632,914 - -0.1 3,632,914 - +0.1 3,632,914 - -1.6 3,632,944 - -2.4 3,632,948 + +1.4 3,632,948 + +0.5 3,632,948 + -2.5 3,632,949 - -0.0 3,632,949 - -0.3 3,633,031 + +0.2 3,633,031 + +0.0 3,633,032 - +0.5 3,633,203 - RR42_RS36450 0.46 +1.5 3,633,386 - RR42_RS36450 0.85 +0.9 3,633,421 + -2.3 3,633,421 + +2.1 3,633,422 - -0.4 3,633,827 + RR42_RS36455 0.30 +0.1 3,633,827 + RR42_RS36455 0.30 +0.3 3,633,828 - RR42_RS36455 0.31 -0.5 3,633,828 - RR42_RS36455 0.31 -0.1 3,633,828 - RR42_RS36455 0.31 -0.0
Or see this region's nucleotide sequence