Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36345 and RR42_RS36350 overlap by 4 nucleotides RR42_RS36350 and RR42_RS36355 are separated by 76 nucleotides RR42_RS36355 and RR42_RS36360 are separated by 56 nucleotides
RR42_RS36345: RR42_RS36345 - hydroxyproline-2-epimerase, at 3,606,032 to 3,607,000
_RS36345
RR42_RS36350: RR42_RS36350 - D-amino acid oxidase, at 3,606,997 to 3,608,097
_RS36350
RR42_RS36355: RR42_RS36355 - (2Fe-2S)-binding protein, at 3,608,174 to 3,608,407
_RS36355
RR42_RS36360: RR42_RS36360 - pyridine nucleotide-disulfide oxidoreductase, at 3,608,464 to 3,609,705
_RS36360
Position (kb)
3606
3607
3608
3609 Strain fitness (log2 ratio)
-2
-1
0
1 at 3606.272 kb on + strand, within RR42_RS36345 at 3606.362 kb on - strand, within RR42_RS36345 at 3606.362 kb on - strand, within RR42_RS36345 at 3606.647 kb on - strand, within RR42_RS36345 at 3606.722 kb on + strand, within RR42_RS36345 at 3606.723 kb on - strand, within RR42_RS36345 at 3606.848 kb on + strand, within RR42_RS36345 at 3606.848 kb on + strand, within RR42_RS36345 at 3606.848 kb on + strand, within RR42_RS36345 at 3606.849 kb on - strand, within RR42_RS36345 at 3606.849 kb on - strand, within RR42_RS36345 at 3606.908 kb on + strand at 3606.909 kb on - strand at 3606.909 kb on - strand at 3607.861 kb on + strand, within RR42_RS36350 at 3607.862 kb on - strand, within RR42_RS36350 at 3607.862 kb on - strand, within RR42_RS36350 at 3608.243 kb on + strand, within RR42_RS36355 at 3608.244 kb on - strand, within RR42_RS36355 at 3608.245 kb on + strand, within RR42_RS36355 at 3608.246 kb on - strand, within RR42_RS36355 at 3608.831 kb on + strand, within RR42_RS36360 at 3608.832 kb on - strand, within RR42_RS36360 at 3608.883 kb on - strand, within RR42_RS36360 at 3608.883 kb on - strand, within RR42_RS36360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,606,272 + RR42_RS36345 0.25 +0.4 3,606,362 - RR42_RS36345 0.34 -1.7 3,606,362 - RR42_RS36345 0.34 -0.1 3,606,647 - RR42_RS36345 0.63 +0.8 3,606,722 + RR42_RS36345 0.71 -0.3 3,606,723 - RR42_RS36345 0.71 +0.1 3,606,848 + RR42_RS36345 0.84 +1.5 3,606,848 + RR42_RS36345 0.84 -1.1 3,606,848 + RR42_RS36345 0.84 +0.8 3,606,849 - RR42_RS36345 0.84 -0.8 3,606,849 - RR42_RS36345 0.84 -0.7 3,606,908 + -0.8 3,606,909 - +1.1 3,606,909 - +1.4 3,607,861 + RR42_RS36350 0.78 -1.3 3,607,862 - RR42_RS36350 0.79 +0.2 3,607,862 - RR42_RS36350 0.79 -1.1 3,608,243 + RR42_RS36355 0.29 -0.3 3,608,244 - RR42_RS36355 0.30 +0.6 3,608,245 + RR42_RS36355 0.30 +0.1 3,608,246 - RR42_RS36355 0.31 +1.6 3,608,831 + RR42_RS36360 0.30 -0.4 3,608,832 - RR42_RS36360 0.30 +1.2 3,608,883 - RR42_RS36360 0.34 -1.0 3,608,883 - RR42_RS36360 0.34 -2.4
Or see this region's nucleotide sequence