Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS36000 and RR42_RS36005 are separated by 82 nucleotides RR42_RS36005 and RR42_RS36010 are separated by 87 nucleotides RR42_RS36010 and RR42_RS36015 are separated by 9 nucleotides
RR42_RS36000: RR42_RS36000 - pilus assembly protein CpaB, at 3,525,105 to 3,526,076
_RS36000
RR42_RS36005: RR42_RS36005 - pilus assembly protein TadG, at 3,526,159 to 3,526,623
_RS36005
RR42_RS36010: RR42_RS36010 - peptidase, at 3,526,711 to 3,527,214
_RS36010
RR42_RS36015: RR42_RS36015 - pilus assembly protein, at 3,527,224 to 3,527,412
_RS36015
Position (kb)
3526
3527 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3525.357 kb on + strand, within RR42_RS36000 at 3525.357 kb on + strand, within RR42_RS36000 at 3525.572 kb on - strand, within RR42_RS36000 at 3525.844 kb on + strand, within RR42_RS36000 at 3525.867 kb on + strand, within RR42_RS36000 at 3525.867 kb on + strand, within RR42_RS36000 at 3525.877 kb on - strand, within RR42_RS36000 at 3525.943 kb on + strand, within RR42_RS36000 at 3526.080 kb on - strand at 3526.080 kb on - strand at 3526.097 kb on - strand at 3526.225 kb on + strand, within RR42_RS36005 at 3526.225 kb on + strand, within RR42_RS36005 at 3526.225 kb on + strand, within RR42_RS36005 at 3526.225 kb on + strand, within RR42_RS36005 at 3526.226 kb on - strand, within RR42_RS36005 at 3526.226 kb on - strand, within RR42_RS36005 at 3526.226 kb on - strand, within RR42_RS36005 at 3526.514 kb on + strand, within RR42_RS36005 at 3526.623 kb on + strand at 3526.623 kb on + strand at 3526.624 kb on - strand at 3526.643 kb on + strand at 3526.712 kb on + strand at 3526.712 kb on + strand at 3526.755 kb on - strand at 3527.019 kb on - strand, within RR42_RS36010 at 3527.226 kb on - strand at 3527.299 kb on + strand, within RR42_RS36015 at 3527.300 kb on - strand, within RR42_RS36015 at 3527.300 kb on - strand, within RR42_RS36015 at 3527.300 kb on - strand, within RR42_RS36015 at 3527.319 kb on + strand, within RR42_RS36015 at 3527.319 kb on + strand, within RR42_RS36015 at 3527.354 kb on + strand, within RR42_RS36015 at 3527.396 kb on - strand at 3527.435 kb on - strand at 3527.493 kb on + strand at 3527.497 kb on - strand at 3527.524 kb on + strand at 3527.527 kb on - strand at 3527.541 kb on + strand at 3527.541 kb on + strand at 3527.541 kb on + strand at 3527.542 kb on - strand at 3527.542 kb on - strand at 3527.542 kb on - strand at 3527.542 kb on - strand at 3527.551 kb on - strand at 3527.566 kb on + strand at 3527.566 kb on + strand at 3527.567 kb on - strand at 3527.569 kb on - strand at 3527.569 kb on - strand at 3527.583 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,525,357 + RR42_RS36000 0.26 -0.4 3,525,357 + RR42_RS36000 0.26 -0.5 3,525,572 - RR42_RS36000 0.48 +1.4 3,525,844 + RR42_RS36000 0.76 +0.4 3,525,867 + RR42_RS36000 0.78 +0.9 3,525,867 + RR42_RS36000 0.78 +0.6 3,525,877 - RR42_RS36000 0.79 +0.6 3,525,943 + RR42_RS36000 0.86 -1.1 3,526,080 - +0.3 3,526,080 - +0.4 3,526,097 - -0.7 3,526,225 + RR42_RS36005 0.14 -1.4 3,526,225 + RR42_RS36005 0.14 -1.0 3,526,225 + RR42_RS36005 0.14 -0.5 3,526,225 + RR42_RS36005 0.14 -1.9 3,526,226 - RR42_RS36005 0.14 +0.9 3,526,226 - RR42_RS36005 0.14 -0.4 3,526,226 - RR42_RS36005 0.14 +1.2 3,526,514 + RR42_RS36005 0.76 -0.3 3,526,623 + +0.2 3,526,623 + -0.6 3,526,624 - +0.7 3,526,643 + +0.3 3,526,712 + -1.0 3,526,712 + +0.1 3,526,755 - +0.3 3,527,019 - RR42_RS36010 0.61 -0.3 3,527,226 - -1.3 3,527,299 + RR42_RS36015 0.40 -2.9 3,527,300 - RR42_RS36015 0.40 -0.3 3,527,300 - RR42_RS36015 0.40 +0.8 3,527,300 - RR42_RS36015 0.40 +1.4 3,527,319 + RR42_RS36015 0.50 +0.0 3,527,319 + RR42_RS36015 0.50 +0.6 3,527,354 + RR42_RS36015 0.69 -0.7 3,527,396 - -0.4 3,527,435 - -0.3 3,527,493 + -0.6 3,527,497 - -1.5 3,527,524 + -0.4 3,527,527 - -0.3 3,527,541 + +1.5 3,527,541 + -2.4 3,527,541 + -1.4 3,527,542 - -3.1 3,527,542 - -1.4 3,527,542 - -2.6 3,527,542 - +0.5 3,527,551 - -1.6 3,527,566 + -1.4 3,527,566 + +0.3 3,527,567 - +0.3 3,527,569 - +0.3 3,527,569 - -0.0 3,527,583 - -0.8
Or see this region's nucleotide sequence