Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35430 and RR42_RS35435 are separated by 680 nucleotides RR42_RS35435 and RR42_RS35440 are separated by 12 nucleotides RR42_RS35440 and RR42_RS35445 are separated by 84 nucleotides
RR42_RS35430: RR42_RS35430 - hypothetical protein, at 3,401,527 to 3,401,907
_RS35430
RR42_RS35435: RR42_RS35435 - NAD(FAD)-dependent dehydrogenase, at 3,402,588 to 3,404,255
_RS35435
RR42_RS35440: RR42_RS35440 - MBL fold metallo-hydrolase, at 3,404,268 to 3,405,128
_RS35440
RR42_RS35445: RR42_RS35445 - ArsR family transcriptional regulator, at 3,405,213 to 3,405,536
_RS35445
Position (kb)
3402
3403
3404
3405 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3401.594 kb on - strand, within RR42_RS35430 at 3401.594 kb on - strand, within RR42_RS35430 at 3401.622 kb on + strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.625 kb on + strand, within RR42_RS35430 at 3401.625 kb on + strand, within RR42_RS35430 at 3401.626 kb on - strand, within RR42_RS35430 at 3401.714 kb on + strand, within RR42_RS35430 at 3401.714 kb on + strand, within RR42_RS35430 at 3401.718 kb on + strand, within RR42_RS35430 at 3401.719 kb on - strand, within RR42_RS35430 at 3401.719 kb on - strand, within RR42_RS35430 at 3401.764 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.831 kb on - strand, within RR42_RS35430 at 3401.859 kb on - strand, within RR42_RS35430 at 3401.859 kb on - strand, within RR42_RS35430 at 3401.871 kb on + strand at 3401.872 kb on - strand at 3402.155 kb on - strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.222 kb on - strand at 3402.222 kb on - strand at 3402.222 kb on - strand at 3402.225 kb on + strand at 3402.226 kb on - strand at 3402.226 kb on - strand at 3402.326 kb on + strand at 3402.326 kb on + strand at 3402.327 kb on - strand at 3402.349 kb on - strand at 3402.381 kb on + strand at 3402.382 kb on - strand at 3402.382 kb on - strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.445 kb on + strand at 3402.445 kb on + strand at 3402.445 kb on + strand at 3402.446 kb on - strand at 3402.446 kb on - strand at 3402.446 kb on - strand at 3402.449 kb on + strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.564 kb on + strand at 3402.564 kb on + strand at 3402.565 kb on - strand at 3402.577 kb on + strand at 3402.578 kb on - strand at 3402.590 kb on - strand at 3402.729 kb on - strand at 3402.729 kb on - strand at 3402.748 kb on + strand at 3402.815 kb on - strand, within RR42_RS35435 at 3402.835 kb on + strand, within RR42_RS35435 at 3402.914 kb on + strand, within RR42_RS35435 at 3402.915 kb on - strand, within RR42_RS35435 at 3402.937 kb on + strand, within RR42_RS35435 at 3402.938 kb on - strand, within RR42_RS35435 at 3402.949 kb on + strand, within RR42_RS35435 at 3402.950 kb on - strand, within RR42_RS35435 at 3402.956 kb on + strand, within RR42_RS35435 at 3402.957 kb on - strand, within RR42_RS35435 at 3402.957 kb on - strand, within RR42_RS35435 at 3403.155 kb on - strand, within RR42_RS35435 at 3403.192 kb on + strand, within RR42_RS35435 at 3403.192 kb on + strand, within RR42_RS35435 at 3403.192 kb on + strand, within RR42_RS35435 at 3403.193 kb on - strand, within RR42_RS35435 at 3403.193 kb on - strand, within RR42_RS35435 at 3403.193 kb on - strand, within RR42_RS35435 at 3403.213 kb on + strand, within RR42_RS35435 at 3403.213 kb on + strand, within RR42_RS35435 at 3403.214 kb on - strand, within RR42_RS35435 at 3403.295 kb on + strand, within RR42_RS35435 at 3403.295 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.309 kb on + strand, within RR42_RS35435 at 3403.310 kb on - strand, within RR42_RS35435 at 3403.310 kb on - strand, within RR42_RS35435 at 3403.310 kb on - strand, within RR42_RS35435 at 3403.310 kb on - strand, within RR42_RS35435 at 3403.409 kb on - strand, within RR42_RS35435 at 3403.509 kb on + strand, within RR42_RS35435 at 3403.525 kb on + strand, within RR42_RS35435 at 3403.693 kb on + strand, within RR42_RS35435 at 3403.694 kb on - strand, within RR42_RS35435 at 3403.696 kb on + strand, within RR42_RS35435 at 3403.697 kb on - strand, within RR42_RS35435 at 3403.732 kb on - strand, within RR42_RS35435 at 3403.860 kb on + strand, within RR42_RS35435 at 3403.861 kb on - strand, within RR42_RS35435 at 3403.905 kb on + strand, within RR42_RS35435 at 3403.988 kb on + strand, within RR42_RS35435 at 3403.988 kb on + strand, within RR42_RS35435 at 3404.194 kb on - strand at 3404.260 kb on + strand at 3404.260 kb on + strand at 3404.261 kb on - strand at 3404.261 kb on - strand at 3404.261 kb on - strand at 3404.472 kb on + strand, within RR42_RS35440 at 3404.575 kb on + strand, within RR42_RS35440 at 3404.576 kb on - strand, within RR42_RS35440 at 3404.576 kb on - strand, within RR42_RS35440 at 3404.675 kb on - strand, within RR42_RS35440 at 3404.692 kb on - strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.693 kb on + strand, within RR42_RS35440 at 3404.694 kb on - strand, within RR42_RS35440 at 3404.888 kb on - strand, within RR42_RS35440 at 3404.888 kb on - strand, within RR42_RS35440 at 3405.019 kb on + strand, within RR42_RS35440 at 3405.020 kb on - strand, within RR42_RS35440 at 3405.020 kb on - strand, within RR42_RS35440 at 3405.068 kb on - strand at 3405.076 kb on + strand at 3405.076 kb on + strand at 3405.076 kb on + strand at 3405.077 kb on - strand at 3405.077 kb on - strand at 3405.077 kb on - strand at 3405.156 kb on + strand at 3405.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,401,594 - RR42_RS35430 0.18 -2.6 3,401,594 - RR42_RS35430 0.18 -3.0 3,401,622 + RR42_RS35430 0.25 +0.2 3,401,623 - RR42_RS35430 0.25 +1.0 3,401,623 - RR42_RS35430 0.25 +1.2 3,401,623 - RR42_RS35430 0.25 -0.2 3,401,625 + RR42_RS35430 0.26 -0.6 3,401,625 + RR42_RS35430 0.26 -1.3 3,401,626 - RR42_RS35430 0.26 +0.5 3,401,714 + RR42_RS35430 0.49 -0.4 3,401,714 + RR42_RS35430 0.49 -1.7 3,401,718 + RR42_RS35430 0.50 -0.7 3,401,719 - RR42_RS35430 0.50 +0.5 3,401,719 - RR42_RS35430 0.50 -1.8 3,401,764 + RR42_RS35430 0.62 -0.3 3,401,780 + RR42_RS35430 0.66 +1.0 3,401,780 + RR42_RS35430 0.66 -1.5 3,401,780 + RR42_RS35430 0.66 +0.5 3,401,780 + RR42_RS35430 0.66 +0.4 3,401,781 - RR42_RS35430 0.67 +0.7 3,401,781 - RR42_RS35430 0.67 -0.8 3,401,781 - RR42_RS35430 0.67 -2.4 3,401,831 - RR42_RS35430 0.80 +1.6 3,401,859 - RR42_RS35430 0.87 +0.1 3,401,859 - RR42_RS35430 0.87 -0.8 3,401,871 + +1.1 3,401,872 - -0.2 3,402,155 - +0.5 3,402,221 + +2.0 3,402,221 + -0.5 3,402,221 + +0.4 3,402,221 + -1.6 3,402,221 + +0.6 3,402,222 - -1.6 3,402,222 - +1.1 3,402,222 - +0.1 3,402,225 + +0.7 3,402,226 - +0.2 3,402,226 - +0.1 3,402,326 + -1.4 3,402,326 + +1.3 3,402,327 - +1.8 3,402,349 - +1.1 3,402,381 + -2.0 3,402,382 - -1.2 3,402,382 - -2.4 3,402,443 + +1.2 3,402,443 + -0.2 3,402,443 + +1.5 3,402,443 + -1.5 3,402,445 + -0.3 3,402,445 + -1.1 3,402,445 + -1.0 3,402,446 - +1.6 3,402,446 - +0.7 3,402,446 - +1.2 3,402,449 + -0.4 3,402,450 - +1.5 3,402,450 - +1.2 3,402,450 - +0.1 3,402,450 - -1.2 3,402,450 - +1.3 3,402,450 - +0.4 3,402,564 + +0.2 3,402,564 + +0.6 3,402,565 - -0.3 3,402,577 + +0.3 3,402,578 - +0.3 3,402,590 - +0.8 3,402,729 - -0.3 3,402,729 - +0.1 3,402,748 + +1.7 3,402,815 - RR42_RS35435 0.14 +0.7 3,402,835 + RR42_RS35435 0.15 -2.7 3,402,914 + RR42_RS35435 0.20 +0.2 3,402,915 - RR42_RS35435 0.20 +2.4 3,402,937 + RR42_RS35435 0.21 +2.7 3,402,938 - RR42_RS35435 0.21 -1.7 3,402,949 + RR42_RS35435 0.22 -0.1 3,402,950 - RR42_RS35435 0.22 -1.2 3,402,956 + RR42_RS35435 0.22 -0.8 3,402,957 - RR42_RS35435 0.22 -1.5 3,402,957 - RR42_RS35435 0.22 +1.1 3,403,155 - RR42_RS35435 0.34 +1.3 3,403,192 + RR42_RS35435 0.36 -0.5 3,403,192 + RR42_RS35435 0.36 -0.2 3,403,192 + RR42_RS35435 0.36 -1.9 3,403,193 - RR42_RS35435 0.36 +1.0 3,403,193 - RR42_RS35435 0.36 +0.2 3,403,193 - RR42_RS35435 0.36 -0.9 3,403,213 + RR42_RS35435 0.37 -1.3 3,403,213 + RR42_RS35435 0.37 +1.2 3,403,214 - RR42_RS35435 0.38 +0.2 3,403,295 + RR42_RS35435 0.42 -0.9 3,403,295 + RR42_RS35435 0.42 +0.1 3,403,309 + RR42_RS35435 0.43 +0.2 3,403,309 + RR42_RS35435 0.43 -1.2 3,403,309 + RR42_RS35435 0.43 +0.7 3,403,309 + RR42_RS35435 0.43 +1.0 3,403,309 + RR42_RS35435 0.43 -1.0 3,403,309 + RR42_RS35435 0.43 +1.1 3,403,309 + RR42_RS35435 0.43 -2.5 3,403,310 - RR42_RS35435 0.43 +0.4 3,403,310 - RR42_RS35435 0.43 +0.5 3,403,310 - RR42_RS35435 0.43 -3.1 3,403,310 - RR42_RS35435 0.43 -2.6 3,403,409 - RR42_RS35435 0.49 -2.4 3,403,509 + RR42_RS35435 0.55 +0.6 3,403,525 + RR42_RS35435 0.56 -1.0 3,403,693 + RR42_RS35435 0.66 -0.6 3,403,694 - RR42_RS35435 0.66 +1.0 3,403,696 + RR42_RS35435 0.66 +0.7 3,403,697 - RR42_RS35435 0.66 +1.8 3,403,732 - RR42_RS35435 0.69 -0.4 3,403,860 + RR42_RS35435 0.76 -0.9 3,403,861 - RR42_RS35435 0.76 -1.7 3,403,905 + RR42_RS35435 0.79 -0.6 3,403,988 + RR42_RS35435 0.84 +1.5 3,403,988 + RR42_RS35435 0.84 -0.7 3,404,194 - +1.1 3,404,260 + -1.9 3,404,260 + +0.2 3,404,261 - -0.5 3,404,261 - -0.7 3,404,261 - +0.9 3,404,472 + RR42_RS35440 0.24 -0.0 3,404,575 + RR42_RS35440 0.36 -2.2 3,404,576 - RR42_RS35440 0.36 +0.1 3,404,576 - RR42_RS35440 0.36 +0.1 3,404,675 - RR42_RS35440 0.47 +0.1 3,404,692 - RR42_RS35440 0.49 -0.8 3,404,693 + RR42_RS35440 0.49 -0.7 3,404,693 + RR42_RS35440 0.49 -1.6 3,404,693 + RR42_RS35440 0.49 +0.9 3,404,694 - RR42_RS35440 0.49 -0.5 3,404,888 - RR42_RS35440 0.72 -0.0 3,404,888 - RR42_RS35440 0.72 -0.9 3,405,019 + RR42_RS35440 0.87 -0.2 3,405,020 - RR42_RS35440 0.87 -0.4 3,405,020 - RR42_RS35440 0.87 +1.1 3,405,068 - -2.4 3,405,076 + +2.0 3,405,076 + -0.3 3,405,076 + +0.8 3,405,077 - -0.9 3,405,077 - +0.6 3,405,077 - -2.2 3,405,156 + +0.4 3,405,157 - +0.4
Or see this region's nucleotide sequence