Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35190 and RR42_RS35195 are separated by 514 nucleotides RR42_RS35195 and RR42_RS35200 are separated by 84 nucleotides
RR42_RS35190: RR42_RS35190 - LacI family transcriptional regulator, at 3,347,520 to 3,348,596
_RS35190
RR42_RS35195: RR42_RS35195 - 2,5-diketo-D-gluconic acid reductase, at 3,349,111 to 3,349,914
_RS35195
RR42_RS35200: RR42_RS35200 - epimerase, at 3,349,999 to 3,350,901
_RS35200
Position (kb)
3349
3350 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3348.303 kb on - strand, within RR42_RS35190 at 3348.303 kb on - strand, within RR42_RS35190 at 3348.303 kb on - strand, within RR42_RS35190 at 3348.373 kb on + strand, within RR42_RS35190 at 3348.373 kb on + strand, within RR42_RS35190 at 3348.374 kb on - strand, within RR42_RS35190 at 3348.374 kb on - strand, within RR42_RS35190 at 3348.374 kb on - strand, within RR42_RS35190 at 3348.374 kb on - strand, within RR42_RS35190 at 3348.558 kb on + strand at 3348.559 kb on - strand at 3348.585 kb on + strand at 3348.585 kb on + strand at 3348.585 kb on + strand at 3348.585 kb on + strand at 3348.585 kb on + strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.586 kb on - strand at 3348.710 kb on + strand at 3348.710 kb on + strand at 3348.710 kb on + strand at 3348.710 kb on + strand at 3348.710 kb on + strand at 3348.711 kb on - strand at 3348.711 kb on - strand at 3348.711 kb on - strand at 3348.711 kb on - strand at 3348.711 kb on - strand at 3348.711 kb on - strand at 3348.711 kb on - strand at 3348.782 kb on + strand at 3348.782 kb on + strand at 3348.783 kb on - strand at 3348.786 kb on - strand at 3348.811 kb on - strand at 3348.829 kb on + strand at 3348.829 kb on + strand at 3348.957 kb on - strand at 3348.957 kb on - strand at 3349.011 kb on + strand at 3349.011 kb on + strand at 3349.012 kb on - strand at 3349.032 kb on + strand at 3349.033 kb on - strand at 3349.062 kb on + strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.071 kb on + strand at 3349.071 kb on + strand at 3349.072 kb on - strand at 3349.382 kb on + strand, within RR42_RS35195 at 3349.382 kb on + strand, within RR42_RS35195 at 3349.382 kb on + strand, within RR42_RS35195 at 3349.382 kb on + strand, within RR42_RS35195 at 3349.382 kb on + strand, within RR42_RS35195 at 3349.383 kb on - strand, within RR42_RS35195 at 3349.799 kb on + strand, within RR42_RS35195 at 3349.799 kb on + strand, within RR42_RS35195 at 3349.800 kb on - strand, within RR42_RS35195 at 3349.887 kb on + strand at 3350.114 kb on + strand, within RR42_RS35200 at 3350.220 kb on - strand, within RR42_RS35200 at 3350.246 kb on + strand, within RR42_RS35200 at 3350.246 kb on + strand, within RR42_RS35200 at 3350.247 kb on - strand, within RR42_RS35200 at 3350.285 kb on + strand, within RR42_RS35200 at 3350.286 kb on - strand, within RR42_RS35200 at 3350.613 kb on + strand, within RR42_RS35200 at 3350.663 kb on - strand, within RR42_RS35200 at 3350.826 kb on + strand at 3350.826 kb on + strand at 3350.827 kb on - strand at 3350.827 kb on - strand at 3350.827 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,348,303 - RR42_RS35190 0.73 +1.0 3,348,303 - RR42_RS35190 0.73 -1.6 3,348,303 - RR42_RS35190 0.73 +1.2 3,348,373 + RR42_RS35190 0.79 +2.4 3,348,373 + RR42_RS35190 0.79 -2.1 3,348,374 - RR42_RS35190 0.79 -0.1 3,348,374 - RR42_RS35190 0.79 -0.8 3,348,374 - RR42_RS35190 0.79 -0.4 3,348,374 - RR42_RS35190 0.79 +0.5 3,348,558 + +0.2 3,348,559 - +0.4 3,348,585 + -2.4 3,348,585 + -0.3 3,348,585 + +0.3 3,348,585 + +1.2 3,348,585 + -0.1 3,348,586 - -0.5 3,348,586 - +0.0 3,348,586 - -1.3 3,348,586 - -0.4 3,348,586 - -2.8 3,348,586 - -1.5 3,348,586 - +0.7 3,348,586 - +0.4 3,348,586 - -1.4 3,348,586 - -2.3 3,348,710 + +0.0 3,348,710 + -2.3 3,348,710 + +0.4 3,348,710 + +1.0 3,348,710 + +0.6 3,348,711 - -1.5 3,348,711 - -1.3 3,348,711 - +0.2 3,348,711 - -0.1 3,348,711 - -0.2 3,348,711 - -0.0 3,348,711 - +1.3 3,348,782 + +0.3 3,348,782 + -0.1 3,348,783 - -0.3 3,348,786 - +0.5 3,348,811 - -2.3 3,348,829 + +0.8 3,348,829 + +0.3 3,348,957 - -1.1 3,348,957 - +0.2 3,349,011 + -0.3 3,349,011 + +0.6 3,349,012 - -1.5 3,349,032 + -1.0 3,349,033 - -0.1 3,349,062 + -1.9 3,349,063 - -0.9 3,349,063 - +0.8 3,349,063 - +0.0 3,349,063 - +1.0 3,349,063 - +1.2 3,349,063 - +0.3 3,349,071 + -0.2 3,349,071 + +0.0 3,349,072 - +1.4 3,349,382 + RR42_RS35195 0.34 -3.5 3,349,382 + RR42_RS35195 0.34 -1.4 3,349,382 + RR42_RS35195 0.34 -2.0 3,349,382 + RR42_RS35195 0.34 -1.1 3,349,382 + RR42_RS35195 0.34 +0.0 3,349,383 - RR42_RS35195 0.34 -1.1 3,349,799 + RR42_RS35195 0.86 -2.1 3,349,799 + RR42_RS35195 0.86 +1.5 3,349,800 - RR42_RS35195 0.86 +0.7 3,349,887 + -1.7 3,350,114 + RR42_RS35200 0.13 +0.1 3,350,220 - RR42_RS35200 0.24 -2.1 3,350,246 + RR42_RS35200 0.27 -0.0 3,350,246 + RR42_RS35200 0.27 +0.5 3,350,247 - RR42_RS35200 0.27 +1.4 3,350,285 + RR42_RS35200 0.32 -0.7 3,350,286 - RR42_RS35200 0.32 +1.7 3,350,613 + RR42_RS35200 0.68 -0.9 3,350,663 - RR42_RS35200 0.74 +2.1 3,350,826 + -0.2 3,350,826 + -2.5 3,350,827 - -1.4 3,350,827 - -0.5 3,350,827 - -1.2
Or see this region's nucleotide sequence