Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34685 and RR42_RS34690 are separated by 210 nucleotides RR42_RS34690 and RR42_RS34695 are separated by 324 nucleotides
RR42_RS34685: RR42_RS34685 - catechol 1,2-dioxygenase, at 3,221,829 to 3,222,695
_RS34685
RR42_RS34690: RR42_RS34690 - permease, at 3,222,906 to 3,223,622
_RS34690
RR42_RS34695: RR42_RS34695 - hypothetical protein, at 3,223,947 to 3,224,699
_RS34695
Position (kb)
3222
3223
3224 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3222.043 kb on + strand, within RR42_RS34685 at 3222.085 kb on + strand, within RR42_RS34685 at 3222.146 kb on - strand, within RR42_RS34685 at 3222.248 kb on - strand, within RR42_RS34685 at 3222.386 kb on - strand, within RR42_RS34685 at 3222.406 kb on + strand, within RR42_RS34685 at 3222.662 kb on + strand at 3222.663 kb on - strand at 3222.856 kb on - strand at 3222.856 kb on - strand at 3222.856 kb on - strand at 3222.862 kb on + strand at 3222.862 kb on + strand at 3222.862 kb on + strand at 3222.862 kb on + strand at 3222.863 kb on - strand at 3223.011 kb on + strand, within RR42_RS34690 at 3223.545 kb on + strand, within RR42_RS34690 at 3223.545 kb on + strand, within RR42_RS34690 at 3223.546 kb on - strand, within RR42_RS34690 at 3223.551 kb on + strand at 3223.551 kb on + strand at 3223.551 kb on + strand at 3223.551 kb on + strand at 3223.552 kb on - strand at 3223.566 kb on + strand at 3223.611 kb on + strand at 3223.612 kb on - strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.613 kb on + strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.614 kb on - strand at 3223.618 kb on - strand at 3223.732 kb on + strand at 3223.733 kb on - strand at 3223.734 kb on + strand at 3223.734 kb on + strand at 3223.735 kb on - strand at 3223.735 kb on - strand at 3223.885 kb on - strand at 3223.938 kb on - strand at 3223.938 kb on - strand at 3223.938 kb on - strand at 3223.938 kb on - strand at 3224.081 kb on + strand, within RR42_RS34695 at 3224.081 kb on + strand, within RR42_RS34695 at 3224.081 kb on + strand, within RR42_RS34695 at 3224.194 kb on + strand, within RR42_RS34695 at 3224.194 kb on + strand, within RR42_RS34695 at 3224.194 kb on + strand, within RR42_RS34695 at 3224.195 kb on - strand, within RR42_RS34695 at 3224.195 kb on - strand, within RR42_RS34695 at 3224.195 kb on - strand, within RR42_RS34695 at 3224.195 kb on - strand, within RR42_RS34695 at 3224.195 kb on - strand, within RR42_RS34695 at 3224.371 kb on - strand, within RR42_RS34695 at 3224.535 kb on + strand, within RR42_RS34695 at 3224.536 kb on - strand, within RR42_RS34695 at 3224.536 kb on - strand, within RR42_RS34695 at 3224.584 kb on + strand, within RR42_RS34695
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,222,043 + RR42_RS34685 0.25 +0.5 3,222,085 + RR42_RS34685 0.30 -1.1 3,222,146 - RR42_RS34685 0.37 -0.2 3,222,248 - RR42_RS34685 0.48 -0.2 3,222,386 - RR42_RS34685 0.64 -0.7 3,222,406 + RR42_RS34685 0.67 +1.8 3,222,662 + +0.2 3,222,663 - +0.6 3,222,856 - +0.5 3,222,856 - +0.2 3,222,856 - +0.8 3,222,862 + -0.2 3,222,862 + -0.9 3,222,862 + +0.0 3,222,862 + -0.6 3,222,863 - -1.4 3,223,011 + RR42_RS34690 0.15 -0.4 3,223,545 + RR42_RS34690 0.89 -0.4 3,223,545 + RR42_RS34690 0.89 -1.0 3,223,546 - RR42_RS34690 0.89 -2.9 3,223,551 + +1.2 3,223,551 + -2.7 3,223,551 + -3.5 3,223,551 + -0.3 3,223,552 - +0.8 3,223,566 + +1.2 3,223,611 + -0.2 3,223,612 - +1.0 3,223,613 + -0.2 3,223,613 + +1.6 3,223,613 + -0.0 3,223,613 + -1.0 3,223,613 + -2.6 3,223,613 + +1.1 3,223,614 - +0.9 3,223,614 - -0.9 3,223,614 - +1.4 3,223,614 - -0.7 3,223,614 - -0.9 3,223,614 - +0.6 3,223,614 - +1.1 3,223,614 - -0.1 3,223,614 - +0.3 3,223,614 - +0.6 3,223,618 - -0.4 3,223,732 + -0.9 3,223,733 - +0.9 3,223,734 + -1.2 3,223,734 + -0.3 3,223,735 - -0.7 3,223,735 - +0.1 3,223,885 - -1.6 3,223,938 - +0.7 3,223,938 - +0.3 3,223,938 - +0.2 3,223,938 - -1.1 3,224,081 + RR42_RS34695 0.18 -2.9 3,224,081 + RR42_RS34695 0.18 -0.0 3,224,081 + RR42_RS34695 0.18 -0.1 3,224,194 + RR42_RS34695 0.33 -0.0 3,224,194 + RR42_RS34695 0.33 -0.3 3,224,194 + RR42_RS34695 0.33 +0.5 3,224,195 - RR42_RS34695 0.33 +2.7 3,224,195 - RR42_RS34695 0.33 -0.2 3,224,195 - RR42_RS34695 0.33 +0.8 3,224,195 - RR42_RS34695 0.33 -0.1 3,224,195 - RR42_RS34695 0.33 +0.4 3,224,371 - RR42_RS34695 0.56 -0.9 3,224,535 + RR42_RS34695 0.78 -1.0 3,224,536 - RR42_RS34695 0.78 -0.6 3,224,536 - RR42_RS34695 0.78 -1.3 3,224,584 + RR42_RS34695 0.85 +0.4
Or see this region's nucleotide sequence