Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34415 and RR42_RS34420 are separated by 42 nucleotides RR42_RS34420 and RR42_RS34425 are separated by 57 nucleotides RR42_RS34425 and RR42_RS34430 are separated by 177 nucleotides
RR42_RS34415: RR42_RS34415 - hypothetical protein, at 3,147,216 to 3,149,609
_RS34415
RR42_RS34420: RR42_RS34420 - Crp/Fnr family transcriptional regulator, at 3,149,652 to 3,150,437
_RS34420
RR42_RS34425: RR42_RS34425 - hypothetical protein, at 3,150,495 to 3,151,652
_RS34425
RR42_RS34430: RR42_RS34430 - oxidoreductase, at 3,151,830 to 3,153,557
_RS34430
Position (kb)
3150
3151
3152 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3149.598 kb on - strand at 3149.734 kb on + strand, within RR42_RS34420 at 3149.735 kb on - strand, within RR42_RS34420 at 3149.749 kb on + strand, within RR42_RS34420 at 3149.833 kb on + strand, within RR42_RS34420 at 3149.991 kb on + strand, within RR42_RS34420 at 3150.052 kb on + strand, within RR42_RS34420 at 3150.053 kb on - strand, within RR42_RS34420 at 3150.286 kb on + strand, within RR42_RS34420 at 3150.496 kb on + strand at 3150.497 kb on - strand at 3150.497 kb on - strand at 3150.497 kb on - strand at 3150.592 kb on + strand at 3150.592 kb on + strand at 3150.592 kb on + strand at 3150.593 kb on - strand at 3150.593 kb on - strand at 3151.226 kb on - strand, within RR42_RS34425 at 3151.228 kb on + strand, within RR42_RS34425 at 3151.228 kb on + strand, within RR42_RS34425 at 3151.358 kb on - strand, within RR42_RS34425 at 3151.759 kb on + strand at 3151.760 kb on - strand at 3151.760 kb on - strand at 3151.770 kb on + strand at 3151.771 kb on - strand at 3151.775 kb on - strand at 3152.060 kb on - strand, within RR42_RS34430 at 3152.153 kb on + strand, within RR42_RS34430 at 3152.153 kb on + strand, within RR42_RS34430 at 3152.267 kb on + strand, within RR42_RS34430 at 3152.267 kb on + strand, within RR42_RS34430 at 3152.268 kb on - strand, within RR42_RS34430 at 3152.268 kb on - strand, within RR42_RS34430 at 3152.495 kb on + strand, within RR42_RS34430 at 3152.496 kb on - strand, within RR42_RS34430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,149,598 - -0.5 3,149,734 + RR42_RS34420 0.10 +0.9 3,149,735 - RR42_RS34420 0.11 +1.3 3,149,749 + RR42_RS34420 0.12 -0.6 3,149,833 + RR42_RS34420 0.23 -1.2 3,149,991 + RR42_RS34420 0.43 -0.8 3,150,052 + RR42_RS34420 0.51 -0.0 3,150,053 - RR42_RS34420 0.51 -2.8 3,150,286 + RR42_RS34420 0.81 -0.7 3,150,496 + -1.5 3,150,497 - -1.4 3,150,497 - +0.4 3,150,497 - +1.0 3,150,592 + +0.7 3,150,592 + +0.5 3,150,592 + -0.1 3,150,593 - -0.4 3,150,593 - -0.2 3,151,226 - RR42_RS34425 0.63 -0.6 3,151,228 + RR42_RS34425 0.63 -0.4 3,151,228 + RR42_RS34425 0.63 -1.0 3,151,358 - RR42_RS34425 0.75 -1.4 3,151,759 + -2.2 3,151,760 - +1.5 3,151,760 - +0.5 3,151,770 + -0.3 3,151,771 - -1.1 3,151,775 - +0.9 3,152,060 - RR42_RS34430 0.13 -0.3 3,152,153 + RR42_RS34430 0.19 +0.4 3,152,153 + RR42_RS34430 0.19 +0.5 3,152,267 + RR42_RS34430 0.25 +1.2 3,152,267 + RR42_RS34430 0.25 -2.2 3,152,268 - RR42_RS34430 0.25 -0.0 3,152,268 - RR42_RS34430 0.25 +1.2 3,152,495 + RR42_RS34430 0.38 -0.7 3,152,496 - RR42_RS34430 0.39 -1.5
Or see this region's nucleotide sequence