Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34365 and RR42_RS34370 are separated by 206 nucleotides RR42_RS34370 and RR42_RS34375 are separated by 48 nucleotides RR42_RS34375 and RR42_RS34380 are separated by 505 nucleotides
RR42_RS34365: RR42_RS34365 - hypothetical protein, at 3,135,083 to 3,135,373
_RS34365
RR42_RS34370: RR42_RS34370 - membrane protein, at 3,135,580 to 3,136,119
_RS34370
RR42_RS34375: RR42_RS34375 - hypothetical protein, at 3,136,168 to 3,136,464
_RS34375
RR42_RS34380: RR42_RS34380 - dihydrolipoamide dehydrogenase, at 3,136,970 to 3,138,379
_RS34380
Position (kb)
3136
3137 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3135.377 kb on + strand at 3135.523 kb on - strand at 3135.527 kb on + strand at 3135.528 kb on - strand at 3135.528 kb on - strand at 3135.528 kb on - strand at 3135.533 kb on + strand at 3135.533 kb on + strand at 3135.534 kb on - strand at 3135.534 kb on - strand at 3135.598 kb on - strand at 3135.677 kb on - strand, within RR42_RS34370 at 3135.677 kb on - strand, within RR42_RS34370 at 3135.677 kb on - strand, within RR42_RS34370 at 3135.684 kb on - strand, within RR42_RS34370 at 3135.715 kb on + strand, within RR42_RS34370 at 3135.716 kb on - strand, within RR42_RS34370 at 3135.947 kb on - strand, within RR42_RS34370 at 3135.961 kb on + strand, within RR42_RS34370 at 3135.962 kb on - strand, within RR42_RS34370 at 3135.976 kb on + strand, within RR42_RS34370 at 3135.976 kb on + strand, within RR42_RS34370 at 3135.976 kb on + strand, within RR42_RS34370 at 3135.977 kb on - strand, within RR42_RS34370 at 3136.040 kb on + strand, within RR42_RS34370 at 3136.058 kb on - strand, within RR42_RS34370 at 3136.175 kb on + strand at 3136.175 kb on + strand at 3136.175 kb on + strand at 3136.175 kb on + strand at 3136.176 kb on - strand at 3136.193 kb on + strand at 3136.303 kb on - strand, within RR42_RS34375 at 3136.304 kb on + strand, within RR42_RS34375 at 3136.304 kb on + strand, within RR42_RS34375 at 3136.304 kb on + strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.305 kb on - strand, within RR42_RS34375 at 3136.728 kb on + strand at 3136.728 kb on + strand at 3136.767 kb on + strand at 3136.767 kb on + strand at 3136.768 kb on - strand at 3136.849 kb on + strand at 3137.040 kb on + strand at 3137.040 kb on + strand at 3137.040 kb on + strand at 3137.083 kb on - strand at 3137.083 kb on - strand at 3137.169 kb on + strand, within RR42_RS34380 at 3137.169 kb on + strand, within RR42_RS34380 at 3137.169 kb on + strand, within RR42_RS34380 at 3137.169 kb on + strand, within RR42_RS34380 at 3137.169 kb on + strand, within RR42_RS34380 at 3137.170 kb on - strand, within RR42_RS34380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,135,377 + +0.7 3,135,523 - +0.1 3,135,527 + +1.0 3,135,528 - +0.7 3,135,528 - -0.3 3,135,528 - -2.5 3,135,533 + +0.8 3,135,533 + -0.4 3,135,534 - +0.6 3,135,534 - -1.6 3,135,598 - -0.3 3,135,677 - RR42_RS34370 0.18 -2.6 3,135,677 - RR42_RS34370 0.18 -2.4 3,135,677 - RR42_RS34370 0.18 -1.6 3,135,684 - RR42_RS34370 0.19 +0.5 3,135,715 + RR42_RS34370 0.25 +0.4 3,135,716 - RR42_RS34370 0.25 -1.2 3,135,947 - RR42_RS34370 0.68 +1.2 3,135,961 + RR42_RS34370 0.71 +0.4 3,135,962 - RR42_RS34370 0.71 +0.7 3,135,976 + RR42_RS34370 0.73 +0.3 3,135,976 + RR42_RS34370 0.73 -0.0 3,135,976 + RR42_RS34370 0.73 +1.8 3,135,977 - RR42_RS34370 0.74 -0.9 3,136,040 + RR42_RS34370 0.85 -0.4 3,136,058 - RR42_RS34370 0.89 +0.4 3,136,175 + +0.5 3,136,175 + +0.7 3,136,175 + -1.6 3,136,175 + -1.0 3,136,176 - -2.6 3,136,193 + -3.2 3,136,303 - RR42_RS34375 0.45 -2.9 3,136,304 + RR42_RS34375 0.46 +0.1 3,136,304 + RR42_RS34375 0.46 -0.3 3,136,304 + RR42_RS34375 0.46 -0.0 3,136,305 - RR42_RS34375 0.46 +0.5 3,136,305 - RR42_RS34375 0.46 +0.9 3,136,305 - RR42_RS34375 0.46 +0.2 3,136,305 - RR42_RS34375 0.46 +0.6 3,136,305 - RR42_RS34375 0.46 +0.3 3,136,305 - RR42_RS34375 0.46 +0.1 3,136,305 - RR42_RS34375 0.46 -1.5 3,136,305 - RR42_RS34375 0.46 -1.2 3,136,305 - RR42_RS34375 0.46 -0.1 3,136,305 - RR42_RS34375 0.46 -1.8 3,136,305 - RR42_RS34375 0.46 +0.6 3,136,728 + +0.5 3,136,728 + +0.4 3,136,767 + +0.8 3,136,767 + +0.2 3,136,768 - -0.0 3,136,849 + +1.2 3,137,040 + -2.6 3,137,040 + -3.3 3,137,040 + -2.0 3,137,083 - +2.5 3,137,083 - -2.1 3,137,169 + RR42_RS34380 0.14 +1.4 3,137,169 + RR42_RS34380 0.14 -0.3 3,137,169 + RR42_RS34380 0.14 +0.1 3,137,169 + RR42_RS34380 0.14 +0.0 3,137,169 + RR42_RS34380 0.14 -3.4 3,137,170 - RR42_RS34380 0.14 +1.7
Or see this region's nucleotide sequence