Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33920 and RR42_RS33925 are separated by 129 nucleotides RR42_RS33925 and RR42_RS33930 are separated by 58 nucleotides
RR42_RS33920: RR42_RS33920 - LysR family transcriptional regulator, at 3,037,448 to 3,038,350
_RS33920
RR42_RS33925: RR42_RS33925 - naphthalene 1,2-dioxygenase, at 3,038,480 to 3,039,466
_RS33925
RR42_RS33930: RR42_RS33930 - salicylate hydroxylase, at 3,039,525 to 3,040,781
_RS33930
Position (kb)
3038
3039
3040 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3037.589 kb on + strand, within RR42_RS33920 at 3037.839 kb on + strand, within RR42_RS33920 at 3037.948 kb on + strand, within RR42_RS33920 at 3037.949 kb on - strand, within RR42_RS33920 at 3037.949 kb on - strand, within RR42_RS33920 at 3038.321 kb on + strand at 3038.321 kb on + strand at 3038.321 kb on + strand at 3038.321 kb on + strand at 3038.322 kb on - strand at 3038.322 kb on - strand at 3038.463 kb on + strand at 3038.464 kb on - strand at 3038.464 kb on - strand at 3038.464 kb on - strand at 3038.487 kb on + strand at 3038.488 kb on - strand at 3038.576 kb on + strand at 3038.579 kb on - strand, within RR42_RS33925 at 3038.910 kb on - strand, within RR42_RS33925 at 3038.987 kb on + strand, within RR42_RS33925 at 3038.987 kb on + strand, within RR42_RS33925 at 3038.987 kb on + strand, within RR42_RS33925 at 3038.988 kb on - strand, within RR42_RS33925 at 3038.988 kb on - strand, within RR42_RS33925 at 3038.988 kb on - strand, within RR42_RS33925 at 3039.050 kb on + strand, within RR42_RS33925 at 3039.050 kb on + strand, within RR42_RS33925 at 3039.051 kb on - strand, within RR42_RS33925 at 3039.544 kb on + strand at 3039.600 kb on + strand at 3039.600 kb on + strand at 3039.600 kb on + strand at 3039.663 kb on + strand, within RR42_RS33930 at 3039.663 kb on + strand, within RR42_RS33930 at 3039.663 kb on + strand, within RR42_RS33930 at 3039.664 kb on - strand, within RR42_RS33930 at 3039.664 kb on - strand, within RR42_RS33930 at 3039.664 kb on - strand, within RR42_RS33930 at 3039.664 kb on - strand, within RR42_RS33930 at 3039.765 kb on + strand, within RR42_RS33930 at 3039.765 kb on + strand, within RR42_RS33930 at 3039.766 kb on - strand, within RR42_RS33930 at 3039.849 kb on + strand, within RR42_RS33930 at 3039.865 kb on - strand, within RR42_RS33930 at 3039.865 kb on - strand, within RR42_RS33930 at 3040.050 kb on + strand, within RR42_RS33930 at 3040.051 kb on - strand, within RR42_RS33930 at 3040.119 kb on + strand, within RR42_RS33930 at 3040.121 kb on + strand, within RR42_RS33930 at 3040.180 kb on - strand, within RR42_RS33930 at 3040.180 kb on - strand, within RR42_RS33930 at 3040.180 kb on - strand, within RR42_RS33930
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,037,589 + RR42_RS33920 0.16 +1.1 3,037,839 + RR42_RS33920 0.43 -0.1 3,037,948 + RR42_RS33920 0.55 +0.4 3,037,949 - RR42_RS33920 0.55 -2.1 3,037,949 - RR42_RS33920 0.55 +0.0 3,038,321 + +0.4 3,038,321 + -1.9 3,038,321 + -1.9 3,038,321 + -3.3 3,038,322 - +0.2 3,038,322 - -0.1 3,038,463 + +0.8 3,038,464 - +1.4 3,038,464 - +1.2 3,038,464 - +0.5 3,038,487 + -0.1 3,038,488 - +0.8 3,038,576 + -0.4 3,038,579 - RR42_RS33925 0.10 -0.8 3,038,910 - RR42_RS33925 0.44 -0.1 3,038,987 + RR42_RS33925 0.51 +0.7 3,038,987 + RR42_RS33925 0.51 +0.8 3,038,987 + RR42_RS33925 0.51 +0.2 3,038,988 - RR42_RS33925 0.51 -1.6 3,038,988 - RR42_RS33925 0.51 -3.1 3,038,988 - RR42_RS33925 0.51 +0.1 3,039,050 + RR42_RS33925 0.58 +0.2 3,039,050 + RR42_RS33925 0.58 +0.2 3,039,051 - RR42_RS33925 0.58 -0.1 3,039,544 + -0.8 3,039,600 + -1.9 3,039,600 + +0.9 3,039,600 + +0.3 3,039,663 + RR42_RS33930 0.11 -1.5 3,039,663 + RR42_RS33930 0.11 -0.5 3,039,663 + RR42_RS33930 0.11 -1.8 3,039,664 - RR42_RS33930 0.11 -0.1 3,039,664 - RR42_RS33930 0.11 -2.4 3,039,664 - RR42_RS33930 0.11 +1.7 3,039,664 - RR42_RS33930 0.11 -2.5 3,039,765 + RR42_RS33930 0.19 -1.1 3,039,765 + RR42_RS33930 0.19 +1.8 3,039,766 - RR42_RS33930 0.19 +0.3 3,039,849 + RR42_RS33930 0.26 +2.0 3,039,865 - RR42_RS33930 0.27 -1.6 3,039,865 - RR42_RS33930 0.27 +1.3 3,040,050 + RR42_RS33930 0.42 +0.4 3,040,051 - RR42_RS33930 0.42 -0.1 3,040,119 + RR42_RS33930 0.47 +0.3 3,040,121 + RR42_RS33930 0.47 -0.2 3,040,180 - RR42_RS33930 0.52 -0.9 3,040,180 - RR42_RS33930 0.52 +0.1 3,040,180 - RR42_RS33930 0.52 -0.1
Or see this region's nucleotide sequence