Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33910 and RR42_RS33915 are separated by 41 nucleotides RR42_RS33915 and RR42_RS33920 are separated by 49 nucleotides RR42_RS33920 and RR42_RS33925 are separated by 129 nucleotides
RR42_RS33910: RR42_RS33910 - LysR family transcriptional regulator, at 3,036,137 to 3,037,030
_RS33910
RR42_RS33915: RR42_RS33915 - DNA-binding protein, at 3,037,072 to 3,037,398
_RS33915
RR42_RS33920: RR42_RS33920 - LysR family transcriptional regulator, at 3,037,448 to 3,038,350
_RS33920
RR42_RS33925: RR42_RS33925 - naphthalene 1,2-dioxygenase, at 3,038,480 to 3,039,466
_RS33925
Position (kb)
3037
3038
3039 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3036.629 kb on + strand, within RR42_RS33910 at 3036.630 kb on - strand, within RR42_RS33910 at 3036.952 kb on + strand at 3036.953 kb on - strand at 3036.972 kb on - strand at 3036.972 kb on - strand at 3036.975 kb on + strand at 3036.976 kb on - strand at 3037.028 kb on + strand at 3037.166 kb on + strand, within RR42_RS33915 at 3037.319 kb on + strand, within RR42_RS33915 at 3037.319 kb on + strand, within RR42_RS33915 at 3037.320 kb on - strand, within RR42_RS33915 at 3037.320 kb on - strand, within RR42_RS33915 at 3037.320 kb on - strand, within RR42_RS33915 at 3037.320 kb on - strand, within RR42_RS33915 at 3037.320 kb on - strand, within RR42_RS33915 at 3037.329 kb on - strand, within RR42_RS33915 at 3037.383 kb on + strand at 3037.386 kb on + strand at 3037.386 kb on + strand at 3037.386 kb on + strand at 3037.386 kb on + strand at 3037.387 kb on - strand at 3037.449 kb on + strand at 3037.450 kb on - strand at 3037.589 kb on + strand, within RR42_RS33920 at 3037.839 kb on + strand, within RR42_RS33920 at 3037.948 kb on + strand, within RR42_RS33920 at 3037.949 kb on - strand, within RR42_RS33920 at 3037.949 kb on - strand, within RR42_RS33920 at 3038.321 kb on + strand at 3038.321 kb on + strand at 3038.321 kb on + strand at 3038.321 kb on + strand at 3038.322 kb on - strand at 3038.322 kb on - strand at 3038.463 kb on + strand at 3038.464 kb on - strand at 3038.464 kb on - strand at 3038.464 kb on - strand at 3038.487 kb on + strand at 3038.488 kb on - strand at 3038.576 kb on + strand at 3038.579 kb on - strand, within RR42_RS33925 at 3038.910 kb on - strand, within RR42_RS33925 at 3038.987 kb on + strand, within RR42_RS33925 at 3038.987 kb on + strand, within RR42_RS33925 at 3038.987 kb on + strand, within RR42_RS33925 at 3038.988 kb on - strand, within RR42_RS33925 at 3038.988 kb on - strand, within RR42_RS33925 at 3038.988 kb on - strand, within RR42_RS33925 at 3039.050 kb on + strand, within RR42_RS33925 at 3039.050 kb on + strand, within RR42_RS33925 at 3039.051 kb on - strand, within RR42_RS33925
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,036,629 + RR42_RS33910 0.55 -3.3 3,036,630 - RR42_RS33910 0.55 -0.8 3,036,952 + -0.5 3,036,953 - +0.9 3,036,972 - +1.8 3,036,972 - -0.6 3,036,975 + -0.1 3,036,976 - +0.9 3,037,028 + +0.1 3,037,166 + RR42_RS33915 0.29 -0.6 3,037,319 + RR42_RS33915 0.76 -1.0 3,037,319 + RR42_RS33915 0.76 +0.5 3,037,320 - RR42_RS33915 0.76 -0.5 3,037,320 - RR42_RS33915 0.76 +0.3 3,037,320 - RR42_RS33915 0.76 -1.0 3,037,320 - RR42_RS33915 0.76 +0.2 3,037,320 - RR42_RS33915 0.76 -2.4 3,037,329 - RR42_RS33915 0.79 +0.1 3,037,383 + +0.8 3,037,386 + +1.7 3,037,386 + -0.1 3,037,386 + -2.0 3,037,386 + +0.5 3,037,387 - -0.8 3,037,449 + -1.9 3,037,450 - -3.4 3,037,589 + RR42_RS33920 0.16 +1.1 3,037,839 + RR42_RS33920 0.43 -0.1 3,037,948 + RR42_RS33920 0.55 +0.4 3,037,949 - RR42_RS33920 0.55 -2.1 3,037,949 - RR42_RS33920 0.55 +0.0 3,038,321 + +0.4 3,038,321 + -1.9 3,038,321 + -1.9 3,038,321 + -3.3 3,038,322 - +0.2 3,038,322 - -0.1 3,038,463 + +0.8 3,038,464 - +1.4 3,038,464 - +1.2 3,038,464 - +0.5 3,038,487 + -0.1 3,038,488 - +0.8 3,038,576 + -0.4 3,038,579 - RR42_RS33925 0.10 -0.8 3,038,910 - RR42_RS33925 0.44 -0.1 3,038,987 + RR42_RS33925 0.51 +0.7 3,038,987 + RR42_RS33925 0.51 +0.8 3,038,987 + RR42_RS33925 0.51 +0.2 3,038,988 - RR42_RS33925 0.51 -1.6 3,038,988 - RR42_RS33925 0.51 -3.1 3,038,988 - RR42_RS33925 0.51 +0.1 3,039,050 + RR42_RS33925 0.58 +0.2 3,039,050 + RR42_RS33925 0.58 +0.2 3,039,051 - RR42_RS33925 0.58 -0.1
Or see this region's nucleotide sequence