Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33895 and RR42_RS33900 overlap by 4 nucleotides RR42_RS33900 and RR42_RS33905 overlap by 7 nucleotides RR42_RS33905 and RR42_RS33910 are separated by 118 nucleotides
RR42_RS33895: RR42_RS33895 - membrane protein, at 3,032,826 to 3,033,743
_RS33895
RR42_RS33900: RR42_RS33900 - membrane protein, at 3,033,740 to 3,033,952
_RS33900
RR42_RS33905: RR42_RS33905 - transporter, at 3,033,946 to 3,036,018
_RS33905
RR42_RS33910: RR42_RS33910 - LysR family transcriptional regulator, at 3,036,137 to 3,037,030
_RS33910
Position (kb)
3033
3034
3035
3036
3037 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3033.161 kb on - strand, within RR42_RS33895 at 3033.172 kb on + strand, within RR42_RS33895 at 3033.173 kb on - strand, within RR42_RS33895 at 3033.223 kb on + strand, within RR42_RS33895 at 3033.224 kb on - strand, within RR42_RS33895 at 3033.649 kb on + strand, within RR42_RS33895 at 3033.649 kb on + strand, within RR42_RS33895 at 3033.650 kb on - strand, within RR42_RS33895 at 3033.650 kb on - strand, within RR42_RS33895 at 3033.650 kb on - strand, within RR42_RS33895 at 3033.792 kb on + strand, within RR42_RS33900 at 3033.792 kb on + strand, within RR42_RS33900 at 3033.792 kb on + strand, within RR42_RS33900 at 3033.801 kb on - strand, within RR42_RS33900 at 3033.835 kb on - strand, within RR42_RS33900 at 3033.927 kb on + strand, within RR42_RS33900 at 3033.948 kb on - strand at 3033.948 kb on - strand at 3034.010 kb on + strand at 3034.011 kb on - strand at 3034.562 kb on + strand, within RR42_RS33905 at 3034.661 kb on + strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.662 kb on - strand, within RR42_RS33905 at 3034.727 kb on + strand, within RR42_RS33905 at 3034.727 kb on + strand, within RR42_RS33905 at 3034.728 kb on - strand, within RR42_RS33905 at 3034.728 kb on - strand, within RR42_RS33905 at 3034.919 kb on + strand, within RR42_RS33905 at 3034.919 kb on + strand, within RR42_RS33905 at 3034.919 kb on + strand, within RR42_RS33905 at 3034.919 kb on + strand, within RR42_RS33905 at 3034.920 kb on - strand, within RR42_RS33905 at 3035.217 kb on + strand, within RR42_RS33905 at 3035.670 kb on + strand, within RR42_RS33905 at 3035.670 kb on + strand, within RR42_RS33905 at 3035.671 kb on - strand, within RR42_RS33905 at 3035.671 kb on - strand, within RR42_RS33905 at 3035.673 kb on - strand, within RR42_RS33905 at 3035.693 kb on + strand, within RR42_RS33905 at 3035.693 kb on + strand, within RR42_RS33905 at 3035.693 kb on + strand, within RR42_RS33905 at 3035.694 kb on - strand, within RR42_RS33905 at 3035.726 kb on + strand, within RR42_RS33905 at 3035.727 kb on - strand, within RR42_RS33905 at 3035.912 kb on + strand at 3035.945 kb on + strand at 3035.946 kb on - strand at 3035.946 kb on - strand at 3035.946 kb on - strand at 3036.013 kb on - strand at 3036.027 kb on + strand at 3036.045 kb on + strand at 3036.046 kb on - strand at 3036.058 kb on + strand at 3036.164 kb on + strand at 3036.165 kb on - strand at 3036.165 kb on - strand at 3036.335 kb on + strand, within RR42_RS33910 at 3036.336 kb on - strand, within RR42_RS33910 at 3036.629 kb on + strand, within RR42_RS33910 at 3036.630 kb on - strand, within RR42_RS33910 at 3036.952 kb on + strand at 3036.953 kb on - strand at 3036.972 kb on - strand at 3036.972 kb on - strand at 3036.975 kb on + strand at 3036.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,033,161 - RR42_RS33895 0.36 -0.2 3,033,172 + RR42_RS33895 0.38 +1.3 3,033,173 - RR42_RS33895 0.38 -0.4 3,033,223 + RR42_RS33895 0.43 -0.9 3,033,224 - RR42_RS33895 0.43 -0.6 3,033,649 + RR42_RS33895 0.90 -1.4 3,033,649 + RR42_RS33895 0.90 +0.7 3,033,650 - RR42_RS33895 0.90 -0.8 3,033,650 - RR42_RS33895 0.90 +2.2 3,033,650 - RR42_RS33895 0.90 +0.9 3,033,792 + RR42_RS33900 0.24 +1.3 3,033,792 + RR42_RS33900 0.24 -1.0 3,033,792 + RR42_RS33900 0.24 -0.2 3,033,801 - RR42_RS33900 0.29 -0.7 3,033,835 - RR42_RS33900 0.45 +0.3 3,033,927 + RR42_RS33900 0.88 +1.2 3,033,948 - -0.3 3,033,948 - +0.5 3,034,010 + +0.4 3,034,011 - +0.2 3,034,562 + RR42_RS33905 0.30 +1.7 3,034,661 + RR42_RS33905 0.34 -2.2 3,034,662 - RR42_RS33905 0.35 +0.2 3,034,662 - RR42_RS33905 0.35 -0.5 3,034,662 - RR42_RS33905 0.35 -0.9 3,034,662 - RR42_RS33905 0.35 +0.2 3,034,662 - RR42_RS33905 0.35 -0.6 3,034,662 - RR42_RS33905 0.35 -1.6 3,034,662 - RR42_RS33905 0.35 +0.5 3,034,727 + RR42_RS33905 0.38 -1.5 3,034,727 + RR42_RS33905 0.38 -0.9 3,034,728 - RR42_RS33905 0.38 +0.5 3,034,728 - RR42_RS33905 0.38 +0.4 3,034,919 + RR42_RS33905 0.47 +0.6 3,034,919 + RR42_RS33905 0.47 +0.6 3,034,919 + RR42_RS33905 0.47 -0.5 3,034,919 + RR42_RS33905 0.47 -2.6 3,034,920 - RR42_RS33905 0.47 -2.5 3,035,217 + RR42_RS33905 0.61 -1.2 3,035,670 + RR42_RS33905 0.83 +0.6 3,035,670 + RR42_RS33905 0.83 +1.1 3,035,671 - RR42_RS33905 0.83 -3.5 3,035,671 - RR42_RS33905 0.83 -0.7 3,035,673 - RR42_RS33905 0.83 -0.1 3,035,693 + RR42_RS33905 0.84 -2.0 3,035,693 + RR42_RS33905 0.84 -0.2 3,035,693 + RR42_RS33905 0.84 -0.6 3,035,694 - RR42_RS33905 0.84 +0.5 3,035,726 + RR42_RS33905 0.86 -1.9 3,035,727 - RR42_RS33905 0.86 -0.0 3,035,912 + -0.0 3,035,945 + +0.4 3,035,946 - +0.1 3,035,946 - -1.1 3,035,946 - -1.5 3,036,013 - -2.2 3,036,027 + +0.9 3,036,045 + +1.8 3,036,046 - +0.0 3,036,058 + +1.1 3,036,164 + +0.3 3,036,165 - -0.1 3,036,165 - +1.4 3,036,335 + RR42_RS33910 0.22 +1.2 3,036,336 - RR42_RS33910 0.22 -0.5 3,036,629 + RR42_RS33910 0.55 -3.3 3,036,630 - RR42_RS33910 0.55 -0.8 3,036,952 + -0.5 3,036,953 - +0.9 3,036,972 - +1.8 3,036,972 - -0.6 3,036,975 + -0.1 3,036,976 - +0.9
Or see this region's nucleotide sequence