Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33750 and RR42_RS33755 are separated by 128 nucleotides RR42_RS33755 and RR42_RS33760 are separated by 147 nucleotides
RR42_RS33750: RR42_RS33750 - MFS transporter, at 2,999,790 to 3,000,764
_RS33750
RR42_RS33755: RR42_RS33755 - tartrate dehydrogenase, at 3,000,893 to 3,001,969
_RS33755
RR42_RS33760: RR42_RS33760 - hypothetical protein, at 3,002,117 to 3,003,001
_RS33760
Position (kb)
3000
3001
3002 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3000.053 kb on + strand, within RR42_RS33750 at 3000.054 kb on - strand, within RR42_RS33750 at 3000.127 kb on + strand, within RR42_RS33750 at 3000.127 kb on + strand, within RR42_RS33750 at 3000.127 kb on + strand, within RR42_RS33750 at 3000.128 kb on - strand, within RR42_RS33750 at 3000.128 kb on - strand, within RR42_RS33750 at 3000.128 kb on - strand, within RR42_RS33750 at 3000.128 kb on - strand, within RR42_RS33750 at 3000.212 kb on - strand, within RR42_RS33750 at 3000.212 kb on - strand, within RR42_RS33750 at 3000.243 kb on + strand, within RR42_RS33750 at 3000.243 kb on + strand, within RR42_RS33750 at 3000.298 kb on + strand, within RR42_RS33750 at 3000.299 kb on - strand, within RR42_RS33750 at 3000.299 kb on - strand, within RR42_RS33750 at 3000.328 kb on + strand, within RR42_RS33750 at 3000.436 kb on + strand, within RR42_RS33750 at 3000.436 kb on + strand, within RR42_RS33750 at 3000.437 kb on - strand, within RR42_RS33750 at 3000.437 kb on - strand, within RR42_RS33750 at 3000.500 kb on - strand, within RR42_RS33750 at 3000.683 kb on - strand at 3000.842 kb on - strand at 3001.362 kb on + strand, within RR42_RS33755 at 3001.363 kb on - strand, within RR42_RS33755 at 3001.564 kb on - strand, within RR42_RS33755 at 3001.658 kb on + strand, within RR42_RS33755 at 3001.658 kb on + strand, within RR42_RS33755 at 3001.659 kb on - strand, within RR42_RS33755 at 3001.803 kb on + strand, within RR42_RS33755 at 3001.803 kb on + strand, within RR42_RS33755 at 3001.804 kb on - strand, within RR42_RS33755 at 3001.804 kb on - strand, within RR42_RS33755 at 3001.806 kb on + strand, within RR42_RS33755 at 3001.807 kb on - strand, within RR42_RS33755 at 3001.807 kb on - strand, within RR42_RS33755 at 3001.821 kb on + strand, within RR42_RS33755 at 3001.821 kb on + strand, within RR42_RS33755 at 3001.821 kb on + strand, within RR42_RS33755 at 3001.822 kb on - strand, within RR42_RS33755 at 3001.824 kb on + strand, within RR42_RS33755 at 3001.825 kb on - strand, within RR42_RS33755 at 3001.959 kb on + strand at 3001.960 kb on - strand at 3001.960 kb on - strand at 3001.960 kb on - strand at 3002.025 kb on + strand at 3002.098 kb on + strand at 3002.309 kb on + strand, within RR42_RS33760 at 3002.310 kb on - strand at 3002.432 kb on + strand, within RR42_RS33760 at 3002.433 kb on - strand, within RR42_RS33760 at 3002.539 kb on - strand, within RR42_RS33760 at 3002.628 kb on - strand, within RR42_RS33760 at 3002.760 kb on + strand, within RR42_RS33760 at 3002.761 kb on - strand, within RR42_RS33760 at 3002.897 kb on + strand, within RR42_RS33760 at 3002.897 kb on + strand, within RR42_RS33760 at 3002.898 kb on - strand, within RR42_RS33760 at 3002.898 kb on - strand, within RR42_RS33760 at 3002.919 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 3,000,053 + RR42_RS33750 0.27 +0.2 3,000,054 - RR42_RS33750 0.27 +0.4 3,000,127 + RR42_RS33750 0.35 -0.7 3,000,127 + RR42_RS33750 0.35 -0.5 3,000,127 + RR42_RS33750 0.35 +1.1 3,000,128 - RR42_RS33750 0.35 -0.6 3,000,128 - RR42_RS33750 0.35 -0.1 3,000,128 - RR42_RS33750 0.35 -0.9 3,000,128 - RR42_RS33750 0.35 +0.3 3,000,212 - RR42_RS33750 0.43 -0.3 3,000,212 - RR42_RS33750 0.43 -0.2 3,000,243 + RR42_RS33750 0.46 -0.4 3,000,243 + RR42_RS33750 0.46 -0.8 3,000,298 + RR42_RS33750 0.52 +0.6 3,000,299 - RR42_RS33750 0.52 +1.2 3,000,299 - RR42_RS33750 0.52 -0.0 3,000,328 + RR42_RS33750 0.55 -0.4 3,000,436 + RR42_RS33750 0.66 +0.7 3,000,436 + RR42_RS33750 0.66 +0.7 3,000,437 - RR42_RS33750 0.66 -0.5 3,000,437 - RR42_RS33750 0.66 -0.7 3,000,500 - RR42_RS33750 0.73 -1.1 3,000,683 - +0.6 3,000,842 - -1.7 3,001,362 + RR42_RS33755 0.44 -1.6 3,001,363 - RR42_RS33755 0.44 -0.7 3,001,564 - RR42_RS33755 0.62 +0.4 3,001,658 + RR42_RS33755 0.71 -1.1 3,001,658 + RR42_RS33755 0.71 -0.9 3,001,659 - RR42_RS33755 0.71 +0.5 3,001,803 + RR42_RS33755 0.84 -0.7 3,001,803 + RR42_RS33755 0.84 -0.1 3,001,804 - RR42_RS33755 0.85 -0.2 3,001,804 - RR42_RS33755 0.85 -2.3 3,001,806 + RR42_RS33755 0.85 -0.7 3,001,807 - RR42_RS33755 0.85 +0.7 3,001,807 - RR42_RS33755 0.85 -2.5 3,001,821 + RR42_RS33755 0.86 +0.4 3,001,821 + RR42_RS33755 0.86 +0.8 3,001,821 + RR42_RS33755 0.86 -1.4 3,001,822 - RR42_RS33755 0.86 -3.2 3,001,824 + RR42_RS33755 0.86 +1.7 3,001,825 - RR42_RS33755 0.87 +0.2 3,001,959 + -1.4 3,001,960 - -3.8 3,001,960 - -3.5 3,001,960 - +0.5 3,002,025 + -1.1 3,002,098 + -0.0 3,002,309 + RR42_RS33760 0.22 +0.7 3,002,310 - +1.5 3,002,432 + RR42_RS33760 0.36 -0.6 3,002,433 - RR42_RS33760 0.36 +1.1 3,002,539 - RR42_RS33760 0.48 -0.5 3,002,628 - RR42_RS33760 0.58 -1.8 3,002,760 + RR42_RS33760 0.73 -0.6 3,002,761 - RR42_RS33760 0.73 -1.4 3,002,897 + RR42_RS33760 0.88 +0.8 3,002,897 + RR42_RS33760 0.88 +0.9 3,002,898 - RR42_RS33760 0.88 +1.6 3,002,898 - RR42_RS33760 0.88 +0.3 3,002,919 + +0.6
Or see this region's nucleotide sequence