Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33710 and RR42_RS33715 are separated by 78 nucleotides RR42_RS33715 and RR42_RS33720 are separated by 44 nucleotides RR42_RS33720 and RR42_RS33725 overlap by 4 nucleotides
RR42_RS33710: RR42_RS33710 - short-chain dehydrogenase, at 2,990,019 to 2,990,768
_RS33710
RR42_RS33715: RR42_RS33715 - MFS transporter, at 2,990,847 to 2,992,172
_RS33715
RR42_RS33720: RR42_RS33720 - transketolase, at 2,992,217 to 2,993,062
_RS33720
RR42_RS33725: RR42_RS33725 - transketolase, at 2,993,059 to 2,994,060
_RS33725
Position (kb)
2990
2991
2992
2993 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2989.940 kb on + strand at 2989.940 kb on + strand at 2990.253 kb on + strand, within RR42_RS33710 at 2990.291 kb on + strand, within RR42_RS33710 at 2990.292 kb on - strand, within RR42_RS33710 at 2990.292 kb on - strand, within RR42_RS33710 at 2990.370 kb on + strand, within RR42_RS33710 at 2990.371 kb on - strand, within RR42_RS33710 at 2990.371 kb on - strand, within RR42_RS33710 at 2990.504 kb on - strand, within RR42_RS33710 at 2990.504 kb on - strand, within RR42_RS33710 at 2990.504 kb on - strand, within RR42_RS33710 at 2990.719 kb on - strand at 2990.719 kb on - strand at 2990.802 kb on + strand at 2990.859 kb on + strand at 2990.860 kb on - strand at 2990.938 kb on + strand at 2990.939 kb on - strand at 2990.950 kb on + strand at 2990.951 kb on - strand at 2990.982 kb on + strand, within RR42_RS33715 at 2991.054 kb on + strand, within RR42_RS33715 at 2991.087 kb on + strand, within RR42_RS33715 at 2991.219 kb on + strand, within RR42_RS33715 at 2991.279 kb on + strand, within RR42_RS33715 at 2991.279 kb on + strand, within RR42_RS33715 at 2991.279 kb on + strand, within RR42_RS33715 at 2991.280 kb on - strand, within RR42_RS33715 at 2991.280 kb on - strand, within RR42_RS33715 at 2991.280 kb on - strand, within RR42_RS33715 at 2991.280 kb on - strand, within RR42_RS33715 at 2991.280 kb on - strand, within RR42_RS33715 at 2991.289 kb on - strand, within RR42_RS33715 at 2991.666 kb on + strand, within RR42_RS33715 at 2991.666 kb on + strand, within RR42_RS33715 at 2991.667 kb on - strand, within RR42_RS33715 at 2991.753 kb on + strand, within RR42_RS33715 at 2991.753 kb on + strand, within RR42_RS33715 at 2991.754 kb on - strand, within RR42_RS33715 at 2992.316 kb on + strand, within RR42_RS33720 at 2992.379 kb on + strand, within RR42_RS33720 at 2992.454 kb on + strand, within RR42_RS33720 at 2992.454 kb on + strand, within RR42_RS33720 at 2992.506 kb on - strand, within RR42_RS33720 at 2992.506 kb on - strand, within RR42_RS33720 at 2992.550 kb on + strand, within RR42_RS33720 at 2992.551 kb on - strand, within RR42_RS33720 at 2992.655 kb on + strand, within RR42_RS33720 at 2992.655 kb on + strand, within RR42_RS33720 at 2992.657 kb on + strand, within RR42_RS33720 at 2992.942 kb on + strand, within RR42_RS33720 at 2992.943 kb on - strand, within RR42_RS33720
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,989,940 + +1.3 2,989,940 + -0.4 2,990,253 + RR42_RS33710 0.31 -1.6 2,990,291 + RR42_RS33710 0.36 -0.3 2,990,292 - RR42_RS33710 0.36 -2.7 2,990,292 - RR42_RS33710 0.36 -0.2 2,990,370 + RR42_RS33710 0.47 +0.3 2,990,371 - RR42_RS33710 0.47 -0.7 2,990,371 - RR42_RS33710 0.47 +0.2 2,990,504 - RR42_RS33710 0.65 -0.4 2,990,504 - RR42_RS33710 0.65 -0.1 2,990,504 - RR42_RS33710 0.65 +1.2 2,990,719 - +0.5 2,990,719 - -0.1 2,990,802 + -1.9 2,990,859 + +0.7 2,990,860 - -1.4 2,990,938 + -0.2 2,990,939 - +1.9 2,990,950 + +0.3 2,990,951 - +0.5 2,990,982 + RR42_RS33715 0.10 -0.3 2,991,054 + RR42_RS33715 0.16 -0.3 2,991,087 + RR42_RS33715 0.18 +0.1 2,991,219 + RR42_RS33715 0.28 -1.1 2,991,279 + RR42_RS33715 0.33 +0.1 2,991,279 + RR42_RS33715 0.33 +0.3 2,991,279 + RR42_RS33715 0.33 -0.5 2,991,280 - RR42_RS33715 0.33 +0.5 2,991,280 - RR42_RS33715 0.33 -0.7 2,991,280 - RR42_RS33715 0.33 -1.9 2,991,280 - RR42_RS33715 0.33 -1.7 2,991,280 - RR42_RS33715 0.33 -0.2 2,991,289 - RR42_RS33715 0.33 -3.5 2,991,666 + RR42_RS33715 0.62 -1.5 2,991,666 + RR42_RS33715 0.62 +1.1 2,991,667 - RR42_RS33715 0.62 -0.6 2,991,753 + RR42_RS33715 0.68 +0.4 2,991,753 + RR42_RS33715 0.68 +0.6 2,991,754 - RR42_RS33715 0.68 +0.1 2,992,316 + RR42_RS33720 0.12 -0.7 2,992,379 + RR42_RS33720 0.19 -0.4 2,992,454 + RR42_RS33720 0.28 -1.7 2,992,454 + RR42_RS33720 0.28 -3.2 2,992,506 - RR42_RS33720 0.34 -0.6 2,992,506 - RR42_RS33720 0.34 -1.0 2,992,550 + RR42_RS33720 0.39 +0.6 2,992,551 - RR42_RS33720 0.39 -1.8 2,992,655 + RR42_RS33720 0.52 +0.5 2,992,655 + RR42_RS33720 0.52 -0.1 2,992,657 + RR42_RS33720 0.52 +1.1 2,992,942 + RR42_RS33720 0.86 +0.5 2,992,943 - RR42_RS33720 0.86 -1.0
Or see this region's nucleotide sequence