Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS33690

Experiment: R2A with D-Cycloserine 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS33685 and RR42_RS33690 are separated by 84 nucleotidesRR42_RS33690 and RR42_RS33695 overlap by 4 nucleotidesRR42_RS33695 and RR42_RS33700 are separated by 85 nucleotides RR42_RS33685: RR42_RS33685 - sulfoacetaldehyde acetyltransferase, at 2,984,771 to 2,986,609 _RS33685 RR42_RS33690: RR42_RS33690 - phosphate acetyltransferase, at 2,986,694 to 2,987,734 _RS33690 RR42_RS33695: RR42_RS33695 - membrane protein, at 2,987,731 to 2,988,501 _RS33695 RR42_RS33700: RR42_RS33700 - tRNA-Arg, at 2,988,587 to 2,988,663 _RS33700 Position (kb) 2986 2987 2988Strain fitness (log2 ratio) -3 -2 -1 0 1at 2986.601 kb on + strandat 2986.601 kb on + strandat 2987.140 kb on - strand, within RR42_RS33690at 2987.540 kb on + strand, within RR42_RS33690at 2987.651 kb on + strandat 2987.782 kb on + strandat 2987.782 kb on + strandat 2987.783 kb on - strandat 2987.783 kb on - strandat 2988.010 kb on + strand, within RR42_RS33695at 2988.011 kb on - strand, within RR42_RS33695at 2988.011 kb on - strand, within RR42_RS33695at 2988.068 kb on - strand, within RR42_RS33695at 2988.435 kb on - strandat 2988.455 kb on - strandat 2988.500 kb on - strandat 2988.500 kb on - strandat 2988.549 kb on + strandat 2988.568 kb on + strandat 2988.570 kb on + strandat 2988.570 kb on + strandat 2988.571 kb on - strandat 2988.613 kb on - strand, within RR42_RS33700at 2988.675 kb on + strandat 2988.708 kb on + strandat 2988.709 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml
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2,986,601 + +0.4
2,986,601 + -3.2
2,987,140 - RR42_RS33690 0.43 +0.8
2,987,540 + RR42_RS33690 0.81 +0.4
2,987,651 + +1.4
2,987,782 + -1.4
2,987,782 + -3.2
2,987,783 - +1.3
2,987,783 - -3.7
2,988,010 + RR42_RS33695 0.36 +0.6
2,988,011 - RR42_RS33695 0.36 -2.2
2,988,011 - RR42_RS33695 0.36 -1.7
2,988,068 - RR42_RS33695 0.44 -0.2
2,988,435 - +0.1
2,988,455 - -1.2
2,988,500 - +0.5
2,988,500 - +0.5
2,988,549 + -1.2
2,988,568 + +0.3
2,988,570 + -0.3
2,988,570 + +0.3
2,988,571 - -0.7
2,988,613 - RR42_RS33700 0.34 -0.4
2,988,675 + -1.2
2,988,708 + -0.1
2,988,709 - -0.9

Or see this region's nucleotide sequence