Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33610 and RR42_RS33615 overlap by 8 nucleotides RR42_RS33615 and RR42_RS33620 overlap by 4 nucleotides RR42_RS33620 and RR42_RS33625 are separated by 131 nucleotides
RR42_RS33610: RR42_RS33610 - ABC transporter ATP-binding protein, at 2,967,725 to 2,968,462
_RS33610
RR42_RS33615: RR42_RS33615 - ABC transporter ATP-binding protein, at 2,968,455 to 2,969,153
_RS33615
RR42_RS33620: RR42_RS33620 - ABC transporter permease, at 2,969,150 to 2,970,022
_RS33620
RR42_RS33625: RR42_RS33625 - ABC transporter permease, at 2,970,154 to 2,970,993
_RS33625
Position (kb)
2969
2970
2971 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2968.430 kb on + strand at 2968.430 kb on + strand at 2968.430 kb on + strand at 2968.460 kb on + strand at 2968.482 kb on + strand at 2968.483 kb on - strand at 2968.486 kb on - strand at 2968.758 kb on + strand, within RR42_RS33615 at 2968.758 kb on + strand, within RR42_RS33615 at 2968.798 kb on - strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.966 kb on - strand, within RR42_RS33615 at 2968.966 kb on - strand, within RR42_RS33615 at 2968.966 kb on - strand, within RR42_RS33615 at 2968.966 kb on - strand, within RR42_RS33615 at 2969.024 kb on + strand, within RR42_RS33615 at 2969.059 kb on - strand, within RR42_RS33615 at 2969.059 kb on - strand, within RR42_RS33615 at 2969.134 kb on - strand at 2969.210 kb on + strand at 2969.210 kb on + strand at 2969.211 kb on - strand at 2969.211 kb on - strand at 2969.378 kb on + strand, within RR42_RS33620 at 2969.378 kb on + strand, within RR42_RS33620 at 2969.488 kb on - strand, within RR42_RS33620 at 2969.702 kb on - strand, within RR42_RS33620 at 2970.172 kb on + strand at 2970.202 kb on - strand at 2970.214 kb on + strand at 2970.247 kb on + strand, within RR42_RS33625 at 2970.265 kb on + strand, within RR42_RS33625 at 2970.332 kb on + strand, within RR42_RS33625 at 2970.541 kb on + strand, within RR42_RS33625 at 2970.577 kb on + strand, within RR42_RS33625 at 2970.578 kb on - strand, within RR42_RS33625 at 2970.578 kb on - strand, within RR42_RS33625 at 2970.587 kb on - strand, within RR42_RS33625 at 2970.607 kb on + strand, within RR42_RS33625 at 2970.607 kb on + strand, within RR42_RS33625 at 2970.608 kb on + strand, within RR42_RS33625 at 2970.608 kb on - strand, within RR42_RS33625 at 2970.608 kb on - strand, within RR42_RS33625 at 2970.694 kb on + strand, within RR42_RS33625 at 2970.694 kb on + strand, within RR42_RS33625 at 2970.694 kb on + strand, within RR42_RS33625 at 2970.694 kb on + strand, within RR42_RS33625 at 2970.695 kb on - strand, within RR42_RS33625 at 2970.695 kb on - strand, within RR42_RS33625 at 2970.695 kb on - strand, within RR42_RS33625 at 2970.716 kb on - strand, within RR42_RS33625 at 2970.716 kb on - strand, within RR42_RS33625 at 2970.736 kb on - strand, within RR42_RS33625 at 2970.796 kb on + strand, within RR42_RS33625 at 2970.866 kb on - strand, within RR42_RS33625 at 2970.866 kb on - strand, within RR42_RS33625 at 2970.908 kb on - strand, within RR42_RS33625 at 2970.952 kb on + strand at 2970.952 kb on + strand at 2970.952 kb on + strand at 2970.953 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,968,430 + -0.4 2,968,430 + +1.5 2,968,430 + -0.9 2,968,460 + +0.4 2,968,482 + -1.5 2,968,483 - +1.2 2,968,486 - +2.5 2,968,758 + RR42_RS33615 0.43 -1.4 2,968,758 + RR42_RS33615 0.43 -0.1 2,968,798 - RR42_RS33615 0.49 -1.1 2,968,965 + RR42_RS33615 0.73 -0.6 2,968,965 + RR42_RS33615 0.73 -0.8 2,968,965 + RR42_RS33615 0.73 +0.4 2,968,965 + RR42_RS33615 0.73 +0.8 2,968,965 + RR42_RS33615 0.73 -1.4 2,968,965 + RR42_RS33615 0.73 +0.4 2,968,965 + RR42_RS33615 0.73 -0.1 2,968,966 - RR42_RS33615 0.73 -0.4 2,968,966 - RR42_RS33615 0.73 -0.3 2,968,966 - RR42_RS33615 0.73 +1.6 2,968,966 - RR42_RS33615 0.73 +1.3 2,969,024 + RR42_RS33615 0.81 +0.9 2,969,059 - RR42_RS33615 0.86 -0.9 2,969,059 - RR42_RS33615 0.86 +0.6 2,969,134 - -0.3 2,969,210 + -1.0 2,969,210 + -2.2 2,969,211 - -3.1 2,969,211 - -1.0 2,969,378 + RR42_RS33620 0.26 +0.5 2,969,378 + RR42_RS33620 0.26 +0.2 2,969,488 - RR42_RS33620 0.39 -2.1 2,969,702 - RR42_RS33620 0.63 +0.9 2,970,172 + -3.1 2,970,202 - +0.2 2,970,214 + -0.5 2,970,247 + RR42_RS33625 0.11 -0.9 2,970,265 + RR42_RS33625 0.13 +0.5 2,970,332 + RR42_RS33625 0.21 -0.1 2,970,541 + RR42_RS33625 0.46 -0.9 2,970,577 + RR42_RS33625 0.50 -0.9 2,970,578 - RR42_RS33625 0.50 -0.0 2,970,578 - RR42_RS33625 0.50 +0.6 2,970,587 - RR42_RS33625 0.52 -0.3 2,970,607 + RR42_RS33625 0.54 +1.3 2,970,607 + RR42_RS33625 0.54 +1.1 2,970,608 + RR42_RS33625 0.54 -0.1 2,970,608 - RR42_RS33625 0.54 +2.3 2,970,608 - RR42_RS33625 0.54 -0.5 2,970,694 + RR42_RS33625 0.64 -2.0 2,970,694 + RR42_RS33625 0.64 +0.0 2,970,694 + RR42_RS33625 0.64 -1.4 2,970,694 + RR42_RS33625 0.64 +1.7 2,970,695 - RR42_RS33625 0.64 -1.1 2,970,695 - RR42_RS33625 0.64 -0.3 2,970,695 - RR42_RS33625 0.64 +0.9 2,970,716 - RR42_RS33625 0.67 -1.4 2,970,716 - RR42_RS33625 0.67 +0.3 2,970,736 - RR42_RS33625 0.69 -1.4 2,970,796 + RR42_RS33625 0.76 +0.2 2,970,866 - RR42_RS33625 0.85 -2.1 2,970,866 - RR42_RS33625 0.85 +0.9 2,970,908 - RR42_RS33625 0.90 -2.1 2,970,952 + -0.9 2,970,952 + +0.6 2,970,952 + +0.6 2,970,953 - -1.1
Or see this region's nucleotide sequence