Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33605 and RR42_RS33610 are separated by 8 nucleotides RR42_RS33610 and RR42_RS33615 overlap by 8 nucleotides RR42_RS33615 and RR42_RS33620 overlap by 4 nucleotides
RR42_RS33605: RR42_RS33605 - ABC transporter substrate-binding protein, at 2,966,439 to 2,967,716
_RS33605
RR42_RS33610: RR42_RS33610 - ABC transporter ATP-binding protein, at 2,967,725 to 2,968,462
_RS33610
RR42_RS33615: RR42_RS33615 - ABC transporter ATP-binding protein, at 2,968,455 to 2,969,153
_RS33615
RR42_RS33620: RR42_RS33620 - ABC transporter permease, at 2,969,150 to 2,970,022
_RS33620
Position (kb)
2967
2968
2969 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2966.741 kb on + strand, within RR42_RS33605 at 2966.741 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.862 kb on + strand, within RR42_RS33605 at 2966.863 kb on - strand, within RR42_RS33605 at 2966.865 kb on + strand, within RR42_RS33605 at 2966.866 kb on - strand, within RR42_RS33605 at 2966.910 kb on + strand, within RR42_RS33605 at 2966.910 kb on + strand, within RR42_RS33605 at 2966.910 kb on + strand, within RR42_RS33605 at 2966.911 kb on - strand, within RR42_RS33605 at 2966.949 kb on + strand, within RR42_RS33605 at 2966.949 kb on + strand, within RR42_RS33605 at 2966.950 kb on - strand, within RR42_RS33605 at 2966.950 kb on - strand, within RR42_RS33605 at 2967.345 kb on + strand, within RR42_RS33605 at 2967.346 kb on - strand, within RR42_RS33605 at 2967.400 kb on - strand, within RR42_RS33605 at 2967.400 kb on - strand, within RR42_RS33605 at 2967.400 kb on - strand, within RR42_RS33605 at 2967.538 kb on - strand, within RR42_RS33605 at 2967.724 kb on + strand at 2967.724 kb on + strand at 2967.725 kb on - strand at 2967.725 kb on - strand at 2968.086 kb on - strand, within RR42_RS33610 at 2968.086 kb on - strand, within RR42_RS33610 at 2968.086 kb on - strand, within RR42_RS33610 at 2968.430 kb on + strand at 2968.430 kb on + strand at 2968.430 kb on + strand at 2968.460 kb on + strand at 2968.482 kb on + strand at 2968.483 kb on - strand at 2968.486 kb on - strand at 2968.758 kb on + strand, within RR42_RS33615 at 2968.758 kb on + strand, within RR42_RS33615 at 2968.798 kb on - strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.965 kb on + strand, within RR42_RS33615 at 2968.966 kb on - strand, within RR42_RS33615 at 2968.966 kb on - strand, within RR42_RS33615 at 2968.966 kb on - strand, within RR42_RS33615 at 2968.966 kb on - strand, within RR42_RS33615 at 2969.024 kb on + strand, within RR42_RS33615 at 2969.059 kb on - strand, within RR42_RS33615 at 2969.059 kb on - strand, within RR42_RS33615 at 2969.134 kb on - strand at 2969.210 kb on + strand at 2969.210 kb on + strand at 2969.211 kb on - strand at 2969.211 kb on - strand at 2969.378 kb on + strand, within RR42_RS33620 at 2969.378 kb on + strand, within RR42_RS33620
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,966,741 + RR42_RS33605 0.24 +1.0 2,966,741 + RR42_RS33605 0.24 -0.8 2,966,862 + RR42_RS33605 0.33 -2.7 2,966,862 + RR42_RS33605 0.33 -1.2 2,966,862 + RR42_RS33605 0.33 +0.9 2,966,862 + RR42_RS33605 0.33 +0.5 2,966,862 + RR42_RS33605 0.33 -0.7 2,966,862 + RR42_RS33605 0.33 -0.1 2,966,863 - RR42_RS33605 0.33 +0.0 2,966,865 + RR42_RS33605 0.33 -1.4 2,966,866 - RR42_RS33605 0.33 -0.9 2,966,910 + RR42_RS33605 0.37 +0.8 2,966,910 + RR42_RS33605 0.37 -0.4 2,966,910 + RR42_RS33605 0.37 +3.0 2,966,911 - RR42_RS33605 0.37 +0.1 2,966,949 + RR42_RS33605 0.40 +0.9 2,966,949 + RR42_RS33605 0.40 +0.9 2,966,950 - RR42_RS33605 0.40 -1.7 2,966,950 - RR42_RS33605 0.40 +1.3 2,967,345 + RR42_RS33605 0.71 -0.2 2,967,346 - RR42_RS33605 0.71 +0.9 2,967,400 - RR42_RS33605 0.75 -0.4 2,967,400 - RR42_RS33605 0.75 +0.4 2,967,400 - RR42_RS33605 0.75 -0.1 2,967,538 - RR42_RS33605 0.86 -0.8 2,967,724 + +0.4 2,967,724 + -1.8 2,967,725 - -0.3 2,967,725 - +2.2 2,968,086 - RR42_RS33610 0.49 +1.4 2,968,086 - RR42_RS33610 0.49 +0.4 2,968,086 - RR42_RS33610 0.49 +0.6 2,968,430 + -0.4 2,968,430 + +1.5 2,968,430 + -0.9 2,968,460 + +0.4 2,968,482 + -1.5 2,968,483 - +1.2 2,968,486 - +2.5 2,968,758 + RR42_RS33615 0.43 -1.4 2,968,758 + RR42_RS33615 0.43 -0.1 2,968,798 - RR42_RS33615 0.49 -1.1 2,968,965 + RR42_RS33615 0.73 -0.6 2,968,965 + RR42_RS33615 0.73 -0.8 2,968,965 + RR42_RS33615 0.73 +0.4 2,968,965 + RR42_RS33615 0.73 +0.8 2,968,965 + RR42_RS33615 0.73 -1.4 2,968,965 + RR42_RS33615 0.73 +0.4 2,968,965 + RR42_RS33615 0.73 -0.1 2,968,966 - RR42_RS33615 0.73 -0.4 2,968,966 - RR42_RS33615 0.73 -0.3 2,968,966 - RR42_RS33615 0.73 +1.6 2,968,966 - RR42_RS33615 0.73 +1.3 2,969,024 + RR42_RS33615 0.81 +0.9 2,969,059 - RR42_RS33615 0.86 -0.9 2,969,059 - RR42_RS33615 0.86 +0.6 2,969,134 - -0.3 2,969,210 + -1.0 2,969,210 + -2.2 2,969,211 - -3.1 2,969,211 - -1.0 2,969,378 + RR42_RS33620 0.26 +0.5 2,969,378 + RR42_RS33620 0.26 +0.2
Or see this region's nucleotide sequence