Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32715 and RR42_RS32720 are separated by 344 nucleotides RR42_RS32720 and RR42_RS32725 are separated by 45 nucleotides
RR42_RS32715: RR42_RS32715 - peroxidase, at 2,734,158 to 2,734,811
_RS32715
RR42_RS32720: RR42_RS32720 - glyoxalase, at 2,735,156 to 2,735,536
_RS32720
RR42_RS32725: RR42_RS32725 - MFS transporter, at 2,735,582 to 2,736,544
_RS32725
Position (kb)
2735
2736 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2734.159 kb on + strand at 2734.216 kb on + strand at 2734.217 kb on - strand at 2734.489 kb on + strand, within RR42_RS32715 at 2734.490 kb on - strand, within RR42_RS32715 at 2734.658 kb on + strand, within RR42_RS32715 at 2734.659 kb on - strand, within RR42_RS32715 at 2734.660 kb on + strand, within RR42_RS32715 at 2734.660 kb on + strand, within RR42_RS32715 at 2734.661 kb on - strand, within RR42_RS32715 at 2734.661 kb on - strand, within RR42_RS32715 at 2734.661 kb on - strand, within RR42_RS32715 at 2734.661 kb on - strand, within RR42_RS32715 at 2734.811 kb on + strand at 2734.812 kb on - strand at 2734.812 kb on - strand at 2734.812 kb on - strand at 2734.914 kb on + strand at 2734.914 kb on + strand at 2734.914 kb on + strand at 2734.914 kb on + strand at 2734.914 kb on + strand at 2734.942 kb on + strand at 2734.942 kb on + strand at 2735.106 kb on + strand at 2735.106 kb on + strand at 2735.106 kb on + strand at 2735.106 kb on + strand at 2735.106 kb on + strand at 2735.107 kb on - strand at 2735.107 kb on - strand at 2735.107 kb on - strand at 2735.112 kb on - strand at 2735.112 kb on - strand at 2735.222 kb on + strand, within RR42_RS32720 at 2735.222 kb on + strand, within RR42_RS32720 at 2735.223 kb on - strand, within RR42_RS32720 at 2735.223 kb on - strand, within RR42_RS32720 at 2735.288 kb on + strand, within RR42_RS32720 at 2735.288 kb on + strand, within RR42_RS32720 at 2735.288 kb on + strand, within RR42_RS32720 at 2735.334 kb on + strand, within RR42_RS32720 at 2736.003 kb on + strand, within RR42_RS32725 at 2736.004 kb on - strand, within RR42_RS32725 at 2736.090 kb on + strand, within RR42_RS32725 at 2736.090 kb on + strand, within RR42_RS32725 at 2736.090 kb on + strand, within RR42_RS32725 at 2736.090 kb on + strand, within RR42_RS32725 at 2736.090 kb on + strand, within RR42_RS32725 at 2736.091 kb on - strand, within RR42_RS32725 at 2736.091 kb on - strand, within RR42_RS32725 at 2736.091 kb on - strand, within RR42_RS32725 at 2736.091 kb on - strand, within RR42_RS32725 at 2736.091 kb on - strand, within RR42_RS32725 at 2736.228 kb on + strand, within RR42_RS32725 at 2736.228 kb on + strand, within RR42_RS32725 at 2736.229 kb on - strand, within RR42_RS32725 at 2736.229 kb on - strand, within RR42_RS32725 at 2736.229 kb on - strand, within RR42_RS32725 at 2736.229 kb on - strand, within RR42_RS32725 at 2736.441 kb on + strand, within RR42_RS32725 at 2736.441 kb on + strand, within RR42_RS32725 at 2736.442 kb on - strand, within RR42_RS32725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,734,159 + +0.4 2,734,216 + -2.2 2,734,217 - -1.0 2,734,489 + RR42_RS32715 0.51 +1.8 2,734,490 - RR42_RS32715 0.51 +0.0 2,734,658 + RR42_RS32715 0.76 -0.7 2,734,659 - RR42_RS32715 0.77 +1.8 2,734,660 + RR42_RS32715 0.77 +0.3 2,734,660 + RR42_RS32715 0.77 +0.7 2,734,661 - RR42_RS32715 0.77 -1.3 2,734,661 - RR42_RS32715 0.77 +0.0 2,734,661 - RR42_RS32715 0.77 +1.1 2,734,661 - RR42_RS32715 0.77 -3.4 2,734,811 + +1.4 2,734,812 - +0.5 2,734,812 - +0.6 2,734,812 - -2.5 2,734,914 + +1.5 2,734,914 + -0.3 2,734,914 + -1.6 2,734,914 + -0.2 2,734,914 + -1.5 2,734,942 + -0.2 2,734,942 + -0.2 2,735,106 + -0.0 2,735,106 + -0.7 2,735,106 + -0.1 2,735,106 + -1.5 2,735,106 + -1.4 2,735,107 - -0.2 2,735,107 - +0.8 2,735,107 - +0.2 2,735,112 - -0.7 2,735,112 - -0.4 2,735,222 + RR42_RS32720 0.17 -1.1 2,735,222 + RR42_RS32720 0.17 -3.9 2,735,223 - RR42_RS32720 0.18 -1.3 2,735,223 - RR42_RS32720 0.18 +0.8 2,735,288 + RR42_RS32720 0.35 -3.7 2,735,288 + RR42_RS32720 0.35 -2.3 2,735,288 + RR42_RS32720 0.35 -0.4 2,735,334 + RR42_RS32720 0.47 -0.6 2,736,003 + RR42_RS32725 0.44 +0.4 2,736,004 - RR42_RS32725 0.44 +0.0 2,736,090 + RR42_RS32725 0.53 -0.9 2,736,090 + RR42_RS32725 0.53 -1.7 2,736,090 + RR42_RS32725 0.53 -0.7 2,736,090 + RR42_RS32725 0.53 +0.6 2,736,090 + RR42_RS32725 0.53 -0.6 2,736,091 - RR42_RS32725 0.53 +0.6 2,736,091 - RR42_RS32725 0.53 +0.8 2,736,091 - RR42_RS32725 0.53 +0.2 2,736,091 - RR42_RS32725 0.53 +1.2 2,736,091 - RR42_RS32725 0.53 +0.9 2,736,228 + RR42_RS32725 0.67 -2.6 2,736,228 + RR42_RS32725 0.67 -1.2 2,736,229 - RR42_RS32725 0.67 +0.3 2,736,229 - RR42_RS32725 0.67 +1.1 2,736,229 - RR42_RS32725 0.67 +0.0 2,736,229 - RR42_RS32725 0.67 +0.4 2,736,441 + RR42_RS32725 0.89 -0.4 2,736,441 + RR42_RS32725 0.89 -0.4 2,736,442 - RR42_RS32725 0.89 +0.3
Or see this region's nucleotide sequence