Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31710 and RR42_RS31715 are separated by 695 nucleotides RR42_RS31715 and RR42_RS31720 are separated by 693 nucleotides RR42_RS31720 and RR42_RS31725 overlap by 4 nucleotides
RR42_RS31710: RR42_RS31710 - HxlR family transcriptional regulator, at 2,492,216 to 2,492,722
_RS31710
RR42_RS31715: RR42_RS31715 - hypothetical protein, at 2,493,418 to 2,493,747
_RS31715
RR42_RS31720: RR42_RS31720 - transcriptional regulator, at 2,494,441 to 2,494,734
_RS31720
RR42_RS31725: RR42_RS31725 - regulator, at 2,494,731 to 2,496,032
_RS31725
Position (kb)
2493
2494 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2492.483 kb on + strand, within RR42_RS31710 at 2492.483 kb on + strand, within RR42_RS31710 at 2492.483 kb on + strand, within RR42_RS31710 at 2492.483 kb on + strand, within RR42_RS31710 at 2492.483 kb on + strand, within RR42_RS31710 at 2492.484 kb on - strand, within RR42_RS31710 at 2492.484 kb on - strand, within RR42_RS31710 at 2492.514 kb on + strand, within RR42_RS31710 at 2492.514 kb on + strand, within RR42_RS31710 at 2492.514 kb on + strand, within RR42_RS31710 at 2492.515 kb on - strand, within RR42_RS31710 at 2492.528 kb on + strand, within RR42_RS31710 at 2492.528 kb on + strand, within RR42_RS31710 at 2492.528 kb on + strand, within RR42_RS31710 at 2492.528 kb on + strand, within RR42_RS31710 at 2492.529 kb on - strand, within RR42_RS31710 at 2492.529 kb on - strand, within RR42_RS31710 at 2492.529 kb on - strand, within RR42_RS31710 at 2492.529 kb on - strand, within RR42_RS31710 at 2492.529 kb on - strand, within RR42_RS31710 at 2492.530 kb on + strand, within RR42_RS31710 at 2492.530 kb on + strand, within RR42_RS31710 at 2492.530 kb on + strand, within RR42_RS31710 at 2492.530 kb on + strand, within RR42_RS31710 at 2492.531 kb on - strand, within RR42_RS31710 at 2492.531 kb on - strand, within RR42_RS31710 at 2492.531 kb on - strand, within RR42_RS31710 at 2492.531 kb on - strand, within RR42_RS31710 at 2492.531 kb on - strand, within RR42_RS31710 at 2492.531 kb on - strand, within RR42_RS31710 at 2492.634 kb on + strand, within RR42_RS31710 at 2492.634 kb on + strand, within RR42_RS31710 at 2492.635 kb on - strand, within RR42_RS31710 at 2492.635 kb on - strand, within RR42_RS31710 at 2492.639 kb on + strand, within RR42_RS31710 at 2492.699 kb on + strand at 2492.716 kb on + strand at 2492.717 kb on - strand at 2492.717 kb on - strand at 2492.720 kb on + strand at 2492.721 kb on - strand at 2492.724 kb on - strand at 2492.728 kb on - strand at 2492.890 kb on - strand at 2492.901 kb on + strand at 2493.057 kb on + strand at 2493.110 kb on + strand at 2493.133 kb on - strand at 2493.153 kb on - strand at 2493.153 kb on - strand at 2493.173 kb on + strand at 2493.419 kb on + strand at 2493.420 kb on - strand at 2493.605 kb on + strand, within RR42_RS31715 at 2493.684 kb on + strand, within RR42_RS31715 at 2493.685 kb on - strand, within RR42_RS31715 at 2493.703 kb on + strand, within RR42_RS31715 at 2493.704 kb on - strand, within RR42_RS31715 at 2493.720 kb on - strand at 2493.720 kb on - strand at 2493.720 kb on - strand at 2493.724 kb on + strand at 2493.724 kb on + strand at 2493.724 kb on + strand at 2493.724 kb on + strand at 2493.724 kb on + strand at 2493.725 kb on - strand at 2493.725 kb on - strand at 2493.725 kb on - strand at 2493.725 kb on - strand at 2493.725 kb on - strand at 2493.725 kb on - strand at 2493.725 kb on - strand at 2493.725 kb on - strand at 2493.725 kb on - strand at 2493.754 kb on - strand at 2493.798 kb on + strand at 2493.799 kb on - strand at 2493.799 kb on - strand at 2493.799 kb on - strand at 2493.814 kb on - strand at 2493.814 kb on - strand at 2493.822 kb on + strand at 2493.822 kb on + strand at 2494.023 kb on + strand at 2494.023 kb on + strand at 2494.234 kb on + strand at 2494.369 kb on - strand at 2494.370 kb on + strand at 2494.425 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,492,483 + RR42_RS31710 0.53 -0.4 2,492,483 + RR42_RS31710 0.53 +0.5 2,492,483 + RR42_RS31710 0.53 -2.6 2,492,483 + RR42_RS31710 0.53 +0.8 2,492,483 + RR42_RS31710 0.53 +1.7 2,492,484 - RR42_RS31710 0.53 -1.0 2,492,484 - RR42_RS31710 0.53 -2.7 2,492,514 + RR42_RS31710 0.59 -2.4 2,492,514 + RR42_RS31710 0.59 +0.4 2,492,514 + RR42_RS31710 0.59 -0.9 2,492,515 - RR42_RS31710 0.59 -0.2 2,492,528 + RR42_RS31710 0.62 +0.4 2,492,528 + RR42_RS31710 0.62 +0.8 2,492,528 + RR42_RS31710 0.62 -1.2 2,492,528 + RR42_RS31710 0.62 -0.6 2,492,529 - RR42_RS31710 0.62 -2.7 2,492,529 - RR42_RS31710 0.62 -1.8 2,492,529 - RR42_RS31710 0.62 -1.5 2,492,529 - RR42_RS31710 0.62 -0.0 2,492,529 - RR42_RS31710 0.62 -3.0 2,492,530 + RR42_RS31710 0.62 -1.2 2,492,530 + RR42_RS31710 0.62 -1.0 2,492,530 + RR42_RS31710 0.62 -1.6 2,492,530 + RR42_RS31710 0.62 -1.8 2,492,531 - RR42_RS31710 0.62 -1.5 2,492,531 - RR42_RS31710 0.62 -1.2 2,492,531 - RR42_RS31710 0.62 -2.4 2,492,531 - RR42_RS31710 0.62 -0.6 2,492,531 - RR42_RS31710 0.62 -0.6 2,492,531 - RR42_RS31710 0.62 -2.7 2,492,634 + RR42_RS31710 0.82 +1.4 2,492,634 + RR42_RS31710 0.82 -1.3 2,492,635 - RR42_RS31710 0.83 -0.8 2,492,635 - RR42_RS31710 0.83 +0.4 2,492,639 + RR42_RS31710 0.83 -0.5 2,492,699 + +0.8 2,492,716 + +1.3 2,492,717 - -0.9 2,492,717 - -0.0 2,492,720 + +0.7 2,492,721 - +0.5 2,492,724 - -2.0 2,492,728 - +0.1 2,492,890 - -0.2 2,492,901 + +0.1 2,493,057 + +1.8 2,493,110 + +0.4 2,493,133 - -1.6 2,493,153 - -1.5 2,493,153 - +1.0 2,493,173 + +0.8 2,493,419 + -0.2 2,493,420 - +1.2 2,493,605 + RR42_RS31715 0.57 -0.7 2,493,684 + RR42_RS31715 0.81 +1.5 2,493,685 - RR42_RS31715 0.81 -2.4 2,493,703 + RR42_RS31715 0.86 -0.9 2,493,704 - RR42_RS31715 0.87 +0.1 2,493,720 - -0.6 2,493,720 - -0.2 2,493,720 - -2.2 2,493,724 + -1.2 2,493,724 + +0.4 2,493,724 + +1.9 2,493,724 + -2.9 2,493,724 + -0.0 2,493,725 - -0.1 2,493,725 - +1.0 2,493,725 - -2.1 2,493,725 - -0.5 2,493,725 - -1.3 2,493,725 - -3.2 2,493,725 - +0.0 2,493,725 - -0.2 2,493,725 - +1.4 2,493,754 - +1.2 2,493,798 + -1.1 2,493,799 - -1.0 2,493,799 - +0.4 2,493,799 - +0.2 2,493,814 - +0.6 2,493,814 - +0.4 2,493,822 + +1.5 2,493,822 + +1.1 2,494,023 + +1.9 2,494,023 + +1.1 2,494,234 + +0.4 2,494,369 - -0.6 2,494,370 + +0.8 2,494,425 + +0.8
Or see this region's nucleotide sequence