Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31350 and RR42_RS31355 are separated by 76 nucleotides RR42_RS31355 and RR42_RS31360 are separated by 118 nucleotides RR42_RS31360 and RR42_RS31365 are separated by 130 nucleotides
RR42_RS31350: RR42_RS31350 - hypothetical protein, at 2,408,533 to 2,409,087
_RS31350
RR42_RS31355: RR42_RS31355 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,409,164 to 2,409,937
_RS31355
RR42_RS31360: RR42_RS31360 - CoA-binding protein, at 2,410,056 to 2,412,185
_RS31360
RR42_RS31365: RR42_RS31365 - formyl-CoA transferase, at 2,412,316 to 2,413,545
_RS31365
Position (kb)
2410
2411
2412
2413 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2409.159 kb on - strand at 2409.351 kb on + strand, within RR42_RS31355 at 2409.531 kb on + strand, within RR42_RS31355 at 2409.541 kb on + strand, within RR42_RS31355 at 2409.541 kb on + strand, within RR42_RS31355 at 2409.542 kb on - strand, within RR42_RS31355 at 2409.631 kb on - strand, within RR42_RS31355 at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.019 kb on + strand at 2410.020 kb on - strand at 2410.020 kb on - strand at 2410.020 kb on - strand at 2410.038 kb on + strand at 2410.088 kb on - strand at 2410.088 kb on - strand at 2410.154 kb on - strand at 2410.154 kb on - strand at 2410.159 kb on + strand at 2410.160 kb on - strand at 2410.160 kb on - strand at 2410.289 kb on + strand, within RR42_RS31360 at 2410.289 kb on + strand, within RR42_RS31360 at 2410.289 kb on + strand, within RR42_RS31360 at 2410.289 kb on + strand, within RR42_RS31360 at 2410.289 kb on + strand, within RR42_RS31360 at 2410.290 kb on - strand, within RR42_RS31360 at 2410.300 kb on + strand, within RR42_RS31360 at 2410.360 kb on + strand, within RR42_RS31360 at 2410.360 kb on + strand, within RR42_RS31360 at 2410.360 kb on + strand, within RR42_RS31360 at 2410.389 kb on - strand, within RR42_RS31360 at 2410.389 kb on - strand, within RR42_RS31360 at 2410.649 kb on - strand, within RR42_RS31360 at 2410.649 kb on - strand, within RR42_RS31360 at 2410.994 kb on - strand, within RR42_RS31360 at 2410.994 kb on - strand, within RR42_RS31360 at 2411.035 kb on + strand, within RR42_RS31360 at 2411.036 kb on - strand, within RR42_RS31360 at 2411.036 kb on - strand, within RR42_RS31360 at 2411.045 kb on - strand, within RR42_RS31360 at 2411.048 kb on + strand, within RR42_RS31360 at 2411.068 kb on - strand, within RR42_RS31360 at 2411.069 kb on + strand, within RR42_RS31360 at 2411.162 kb on + strand, within RR42_RS31360 at 2411.162 kb on + strand, within RR42_RS31360 at 2411.309 kb on - strand, within RR42_RS31360 at 2411.876 kb on - strand, within RR42_RS31360 at 2411.876 kb on - strand, within RR42_RS31360 at 2412.082 kb on + strand at 2412.082 kb on + strand at 2412.295 kb on - strand at 2412.390 kb on + strand at 2412.444 kb on + strand, within RR42_RS31365 at 2412.467 kb on + strand, within RR42_RS31365 at 2412.516 kb on - strand, within RR42_RS31365 at 2412.525 kb on + strand, within RR42_RS31365 at 2412.525 kb on + strand, within RR42_RS31365 at 2412.525 kb on + strand, within RR42_RS31365 at 2412.768 kb on - strand, within RR42_RS31365 at 2412.768 kb on - strand, within RR42_RS31365 at 2412.872 kb on + strand, within RR42_RS31365 at 2412.872 kb on + strand, within RR42_RS31365 at 2413.123 kb on + strand, within RR42_RS31365 at 2413.124 kb on - strand, within RR42_RS31365 at 2413.184 kb on + strand, within RR42_RS31365 at 2413.184 kb on + strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365 at 2413.185 kb on - strand, within RR42_RS31365
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,409,159 - -1.8 2,409,351 + RR42_RS31355 0.24 -0.3 2,409,531 + RR42_RS31355 0.47 +1.2 2,409,541 + RR42_RS31355 0.49 -1.4 2,409,541 + RR42_RS31355 0.49 -0.1 2,409,542 - RR42_RS31355 0.49 +0.2 2,409,631 - RR42_RS31355 0.60 +1.1 2,410,019 + -0.5 2,410,019 + +0.5 2,410,019 + +0.8 2,410,019 + -3.2 2,410,019 + +0.7 2,410,019 + +1.0 2,410,020 - -2.3 2,410,020 - +0.4 2,410,020 - +0.0 2,410,038 + +0.4 2,410,088 - +1.5 2,410,088 - -0.2 2,410,154 - +0.8 2,410,154 - -0.1 2,410,159 + +0.4 2,410,160 - +0.5 2,410,160 - +0.0 2,410,289 + RR42_RS31360 0.11 -2.5 2,410,289 + RR42_RS31360 0.11 +0.1 2,410,289 + RR42_RS31360 0.11 +0.2 2,410,289 + RR42_RS31360 0.11 -2.3 2,410,289 + RR42_RS31360 0.11 -0.5 2,410,290 - RR42_RS31360 0.11 -0.9 2,410,300 + RR42_RS31360 0.11 -0.8 2,410,360 + RR42_RS31360 0.14 +1.4 2,410,360 + RR42_RS31360 0.14 -3.8 2,410,360 + RR42_RS31360 0.14 +0.7 2,410,389 - RR42_RS31360 0.16 +0.8 2,410,389 - RR42_RS31360 0.16 -0.3 2,410,649 - RR42_RS31360 0.28 -0.8 2,410,649 - RR42_RS31360 0.28 -0.1 2,410,994 - RR42_RS31360 0.44 -0.7 2,410,994 - RR42_RS31360 0.44 +1.4 2,411,035 + RR42_RS31360 0.46 -0.3 2,411,036 - RR42_RS31360 0.46 +1.7 2,411,036 - RR42_RS31360 0.46 -0.3 2,411,045 - RR42_RS31360 0.46 +0.4 2,411,048 + RR42_RS31360 0.47 -2.1 2,411,068 - RR42_RS31360 0.48 -0.9 2,411,069 + RR42_RS31360 0.48 -2.3 2,411,162 + RR42_RS31360 0.52 -0.8 2,411,162 + RR42_RS31360 0.52 +0.1 2,411,309 - RR42_RS31360 0.59 -0.6 2,411,876 - RR42_RS31360 0.85 +0.4 2,411,876 - RR42_RS31360 0.85 -1.2 2,412,082 + -1.0 2,412,082 + +0.5 2,412,295 - -0.9 2,412,390 + -0.3 2,412,444 + RR42_RS31365 0.10 +1.2 2,412,467 + RR42_RS31365 0.12 +0.2 2,412,516 - RR42_RS31365 0.16 -0.4 2,412,525 + RR42_RS31365 0.17 -3.5 2,412,525 + RR42_RS31365 0.17 +0.2 2,412,525 + RR42_RS31365 0.17 -1.0 2,412,768 - RR42_RS31365 0.37 -0.6 2,412,768 - RR42_RS31365 0.37 -0.1 2,412,872 + RR42_RS31365 0.45 -0.3 2,412,872 + RR42_RS31365 0.45 -0.4 2,413,123 + RR42_RS31365 0.66 -0.3 2,413,124 - RR42_RS31365 0.66 +0.0 2,413,184 + RR42_RS31365 0.71 -3.0 2,413,184 + RR42_RS31365 0.71 +0.4 2,413,185 - RR42_RS31365 0.71 -2.1 2,413,185 - RR42_RS31365 0.71 -0.1 2,413,185 - RR42_RS31365 0.71 -0.9 2,413,185 - RR42_RS31365 0.71 +0.1 2,413,185 - RR42_RS31365 0.71 +1.0
Or see this region's nucleotide sequence