Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31325 and RR42_RS31330 are separated by 138 nucleotides RR42_RS31330 and RR42_RS31335 are separated by 126 nucleotides
RR42_RS31325: RR42_RS31325 - oxalate:formate antiporter, at 2,404,070 to 2,404,291
_RS31325
RR42_RS31330: RR42_RS31330 - LysR family transcriptional regulator, at 2,404,430 to 2,405,341
_RS31330
RR42_RS31335: RR42_RS31335 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,405,468 to 2,406,808
_RS31335
Position (kb)
2404
2405
2406 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2403.442 kb on + strand at 2403.442 kb on + strand at 2403.443 kb on - strand at 2403.505 kb on + strand at 2403.506 kb on - strand at 2403.525 kb on + strand at 2403.564 kb on + strand at 2403.712 kb on - strand at 2403.849 kb on + strand at 2403.850 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2404.216 kb on + strand, within RR42_RS31325 at 2404.332 kb on - strand at 2404.333 kb on + strand at 2404.334 kb on - strand at 2404.436 kb on + strand at 2404.870 kb on - strand, within RR42_RS31330 at 2405.335 kb on + strand at 2405.356 kb on + strand at 2405.356 kb on + strand at 2405.356 kb on - strand at 2405.357 kb on - strand at 2405.417 kb on + strand at 2405.503 kb on - strand at 2405.503 kb on - strand at 2405.525 kb on + strand at 2405.626 kb on + strand, within RR42_RS31335 at 2405.707 kb on - strand, within RR42_RS31335 at 2405.749 kb on + strand, within RR42_RS31335 at 2405.750 kb on - strand, within RR42_RS31335 at 2405.887 kb on + strand, within RR42_RS31335 at 2405.887 kb on + strand, within RR42_RS31335 at 2405.888 kb on - strand, within RR42_RS31335 at 2406.111 kb on + strand, within RR42_RS31335 at 2406.111 kb on + strand, within RR42_RS31335 at 2406.111 kb on + strand, within RR42_RS31335 at 2406.111 kb on + strand, within RR42_RS31335 at 2406.111 kb on + strand, within RR42_RS31335 at 2406.112 kb on - strand, within RR42_RS31335 at 2406.112 kb on - strand, within RR42_RS31335 at 2406.112 kb on - strand, within RR42_RS31335 at 2406.316 kb on + strand, within RR42_RS31335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,403,442 + +0.7 2,403,442 + +0.6 2,403,443 - -2.0 2,403,505 + +0.9 2,403,506 - +0.9 2,403,525 + -0.6 2,403,564 + +0.5 2,403,712 - -1.5 2,403,849 + +0.8 2,403,850 - -0.6 2,403,944 - +0.6 2,403,944 - -0.4 2,403,944 - -0.2 2,403,944 - +1.8 2,403,944 - +1.0 2,403,944 - +0.8 2,404,216 + RR42_RS31325 0.66 -1.5 2,404,332 - +0.9 2,404,333 + -2.4 2,404,334 - -0.4 2,404,436 + +1.0 2,404,870 - RR42_RS31330 0.48 -1.5 2,405,335 + +0.2 2,405,356 + -2.2 2,405,356 + -2.1 2,405,356 - -0.6 2,405,357 - +0.1 2,405,417 + -2.1 2,405,503 - -1.8 2,405,503 - -1.3 2,405,525 + -0.3 2,405,626 + RR42_RS31335 0.12 +1.0 2,405,707 - RR42_RS31335 0.18 -0.9 2,405,749 + RR42_RS31335 0.21 -0.9 2,405,750 - RR42_RS31335 0.21 -0.7 2,405,887 + RR42_RS31335 0.31 +0.6 2,405,887 + RR42_RS31335 0.31 -2.9 2,405,888 - RR42_RS31335 0.31 -0.1 2,406,111 + RR42_RS31335 0.48 -0.5 2,406,111 + RR42_RS31335 0.48 +0.9 2,406,111 + RR42_RS31335 0.48 -1.6 2,406,111 + RR42_RS31335 0.48 -1.8 2,406,111 + RR42_RS31335 0.48 +1.0 2,406,112 - RR42_RS31335 0.48 +0.1 2,406,112 - RR42_RS31335 0.48 +0.1 2,406,112 - RR42_RS31335 0.48 -0.5 2,406,316 + RR42_RS31335 0.63 +2.5
Or see this region's nucleotide sequence