Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31275 and RR42_RS31280 are separated by 16 nucleotides RR42_RS31280 and RR42_RS31285 are separated by 528 nucleotides RR42_RS31285 and RR42_RS31290 overlap by 96 nucleotides
RR42_RS31275: RR42_RS31275 - Homogentisate 1,2-dioxygenase (EC 1.13.11.5) (from data) , at 2,391,461 to 2,392,810
_RS31275
RR42_RS31280: RR42_RS31280 - hypothetical protein, at 2,392,827 to 2,393,054
_RS31280
RR42_RS31285: RR42_RS31285 - XRE family transcriptional regulator, at 2,393,583 to 2,393,816
_RS31285
RR42_RS31290: RR42_RS31290 - XRE family transcriptional regulator, at 2,393,721 to 2,393,975
_RS31290
Position (kb)
2392
2393
2394 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2391.928 kb on + strand, within RR42_RS31275 at 2391.929 kb on - strand, within RR42_RS31275 at 2391.954 kb on + strand, within RR42_RS31275 at 2391.963 kb on + strand, within RR42_RS31275 at 2391.964 kb on - strand, within RR42_RS31275 at 2392.071 kb on + strand, within RR42_RS31275 at 2392.071 kb on + strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.072 kb on - strand, within RR42_RS31275 at 2392.161 kb on + strand, within RR42_RS31275 at 2392.161 kb on + strand, within RR42_RS31275 at 2392.162 kb on - strand, within RR42_RS31275 at 2392.174 kb on + strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.175 kb on - strand, within RR42_RS31275 at 2392.176 kb on + strand, within RR42_RS31275 at 2392.369 kb on - strand, within RR42_RS31275 at 2392.489 kb on + strand, within RR42_RS31275 at 2392.647 kb on + strand, within RR42_RS31275 at 2392.647 kb on + strand, within RR42_RS31275 at 2392.648 kb on - strand, within RR42_RS31275 at 2392.657 kb on - strand, within RR42_RS31275 at 2392.744 kb on - strand at 2392.828 kb on + strand at 2392.828 kb on + strand at 2392.829 kb on - strand at 2392.829 kb on - strand at 2392.829 kb on - strand at 2393.008 kb on + strand, within RR42_RS31280 at 2393.008 kb on + strand, within RR42_RS31280 at 2393.008 kb on + strand, within RR42_RS31280 at 2393.009 kb on - strand, within RR42_RS31280 at 2393.009 kb on - strand, within RR42_RS31280 at 2393.009 kb on - strand, within RR42_RS31280 at 2393.043 kb on - strand at 2393.179 kb on + strand at 2393.209 kb on + strand at 2393.210 kb on - strand at 2393.219 kb on + strand at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.253 kb on + strand at 2393.254 kb on - strand at 2393.254 kb on - strand at 2393.261 kb on + strand at 2393.261 kb on + strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.262 kb on - strand at 2393.656 kb on - strand, within RR42_RS31285 at 2393.730 kb on - strand at 2393.730 kb on - strand at 2393.852 kb on - strand, within RR42_RS31290 at 2393.852 kb on - strand, within RR42_RS31290 at 2393.966 kb on + strand at 2393.967 kb on - strand at 2394.028 kb on + strand at 2394.028 kb on + strand at 2394.029 kb on - strand at 2394.049 kb on + strand at 2394.049 kb on + strand at 2394.050 kb on - strand at 2394.050 kb on - strand at 2394.050 kb on - strand at 2394.053 kb on + strand at 2394.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,391,928 + RR42_RS31275 0.35 +1.2 2,391,929 - RR42_RS31275 0.35 +0.5 2,391,954 + RR42_RS31275 0.37 +1.5 2,391,963 + RR42_RS31275 0.37 +0.8 2,391,964 - RR42_RS31275 0.37 -1.1 2,392,071 + RR42_RS31275 0.45 +0.0 2,392,071 + RR42_RS31275 0.45 +1.8 2,392,072 - RR42_RS31275 0.45 -0.4 2,392,072 - RR42_RS31275 0.45 +0.1 2,392,072 - RR42_RS31275 0.45 +1.4 2,392,161 + RR42_RS31275 0.52 -0.6 2,392,161 + RR42_RS31275 0.52 +0.7 2,392,162 - RR42_RS31275 0.52 +0.7 2,392,174 + RR42_RS31275 0.53 +0.7 2,392,175 - RR42_RS31275 0.53 +1.3 2,392,175 - RR42_RS31275 0.53 -1.6 2,392,175 - RR42_RS31275 0.53 -0.9 2,392,176 + RR42_RS31275 0.53 +1.5 2,392,369 - RR42_RS31275 0.67 +0.5 2,392,489 + RR42_RS31275 0.76 -1.0 2,392,647 + RR42_RS31275 0.88 +0.6 2,392,647 + RR42_RS31275 0.88 -2.3 2,392,648 - RR42_RS31275 0.88 +0.5 2,392,657 - RR42_RS31275 0.89 +0.4 2,392,744 - +0.2 2,392,828 + +1.4 2,392,828 + +0.4 2,392,829 - +1.3 2,392,829 - -0.4 2,392,829 - +0.5 2,393,008 + RR42_RS31280 0.79 +1.9 2,393,008 + RR42_RS31280 0.79 +2.7 2,393,008 + RR42_RS31280 0.79 +0.3 2,393,009 - RR42_RS31280 0.80 +0.4 2,393,009 - RR42_RS31280 0.80 -0.1 2,393,009 - RR42_RS31280 0.80 +0.5 2,393,043 - -0.8 2,393,179 + +1.3 2,393,209 + +1.0 2,393,210 - +0.1 2,393,219 + -0.7 2,393,253 + +0.1 2,393,253 + -2.8 2,393,253 + -1.3 2,393,253 + +0.2 2,393,254 - +0.4 2,393,254 - +0.8 2,393,261 + +0.8 2,393,261 + -1.6 2,393,262 - +0.5 2,393,262 - +1.1 2,393,262 - +0.5 2,393,262 - +0.0 2,393,262 - +0.1 2,393,262 - -4.4 2,393,262 - -0.6 2,393,262 - +0.0 2,393,656 - RR42_RS31285 0.31 +1.3 2,393,730 - +1.4 2,393,730 - +0.7 2,393,852 - RR42_RS31290 0.51 +1.3 2,393,852 - RR42_RS31290 0.51 +1.1 2,393,966 + +2.6 2,393,967 - +0.6 2,394,028 + +1.8 2,394,028 + +0.9 2,394,029 - +0.2 2,394,049 + +2.0 2,394,049 + +1.9 2,394,050 - -0.0 2,394,050 - -0.3 2,394,050 - -0.8 2,394,053 + +2.1 2,394,054 - +0.3
Or see this region's nucleotide sequence