Experiment: R2A with D-Cycloserine 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31255 and RR42_RS31260 overlap by 14 nucleotides RR42_RS31260 and RR42_RS31265 overlap by 8 nucleotides RR42_RS31265 and RR42_RS31270 are separated by 622 nucleotides
RR42_RS31255: RR42_RS31255 - acyl carrier protein, at 2,387,711 to 2,387,986
_RS31255
RR42_RS31260: RR42_RS31260 - acyltransferase, at 2,387,973 to 2,388,749
_RS31260
RR42_RS31265: RR42_RS31265 - hypothetical protein, at 2,388,742 to 2,389,470
_RS31265
RR42_RS31270: RR42_RS31270 - fumarylacetoacetase, at 2,390,093 to 2,391,355
_RS31270
Position (kb)
2388
2389
2390 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2388.016 kb on - strand at 2388.109 kb on + strand, within RR42_RS31260 at 2388.109 kb on + strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.110 kb on - strand, within RR42_RS31260 at 2388.358 kb on + strand, within RR42_RS31260 at 2388.359 kb on - strand, within RR42_RS31260 at 2388.533 kb on - strand, within RR42_RS31260 at 2388.583 kb on + strand, within RR42_RS31260 at 2388.584 kb on - strand, within RR42_RS31260 at 2388.974 kb on + strand, within RR42_RS31265 at 2388.974 kb on + strand, within RR42_RS31265 at 2388.974 kb on + strand, within RR42_RS31265 at 2388.975 kb on - strand, within RR42_RS31265 at 2388.998 kb on + strand, within RR42_RS31265 at 2388.998 kb on + strand, within RR42_RS31265 at 2388.999 kb on - strand, within RR42_RS31265 at 2389.127 kb on + strand, within RR42_RS31265 at 2389.147 kb on - strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.286 kb on + strand, within RR42_RS31265 at 2389.287 kb on - strand, within RR42_RS31265 at 2389.427 kb on - strand at 2389.427 kb on - strand at 2389.427 kb on - strand at 2389.487 kb on + strand at 2389.488 kb on - strand at 2389.488 kb on - strand at 2389.750 kb on - strand at 2389.762 kb on - strand at 2389.830 kb on + strand at 2389.830 kb on + strand at 2389.831 kb on - strand at 2389.831 kb on - strand at 2389.831 kb on - strand at 2389.831 kb on - strand at 2389.920 kb on + strand at 2389.921 kb on - strand at 2389.972 kb on - strand at 2390.037 kb on - strand at 2390.094 kb on + strand at 2390.376 kb on + strand, within RR42_RS31270 at 2390.376 kb on + strand, within RR42_RS31270 at 2390.376 kb on + strand, within RR42_RS31270 at 2390.376 kb on + strand, within RR42_RS31270 at 2390.377 kb on - strand, within RR42_RS31270 at 2390.377 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270 at 2390.461 kb on - strand, within RR42_RS31270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.5 mg/ml remove 2,388,016 - -0.3 2,388,109 + RR42_RS31260 0.18 +0.6 2,388,109 + RR42_RS31260 0.18 -0.2 2,388,110 - RR42_RS31260 0.18 -1.6 2,388,110 - RR42_RS31260 0.18 +0.4 2,388,110 - RR42_RS31260 0.18 +0.8 2,388,110 - RR42_RS31260 0.18 +0.1 2,388,358 + RR42_RS31260 0.50 -1.6 2,388,359 - RR42_RS31260 0.50 -2.1 2,388,533 - RR42_RS31260 0.72 -1.9 2,388,583 + RR42_RS31260 0.79 -0.6 2,388,584 - RR42_RS31260 0.79 -0.3 2,388,974 + RR42_RS31265 0.32 +0.1 2,388,974 + RR42_RS31265 0.32 -1.0 2,388,974 + RR42_RS31265 0.32 -0.9 2,388,975 - RR42_RS31265 0.32 +0.4 2,388,998 + RR42_RS31265 0.35 +0.6 2,388,998 + RR42_RS31265 0.35 -1.6 2,388,999 - RR42_RS31265 0.35 -1.6 2,389,127 + RR42_RS31265 0.53 +0.3 2,389,147 - RR42_RS31265 0.56 +1.7 2,389,286 + RR42_RS31265 0.75 -1.8 2,389,286 + RR42_RS31265 0.75 -0.3 2,389,286 + RR42_RS31265 0.75 +1.5 2,389,287 - RR42_RS31265 0.75 -0.7 2,389,427 - -3.2 2,389,427 - +0.3 2,389,427 - +1.3 2,389,487 + -2.6 2,389,488 - +1.1 2,389,488 - -1.9 2,389,750 - +1.8 2,389,762 - +0.0 2,389,830 + +0.3 2,389,830 + +0.2 2,389,831 - -1.5 2,389,831 - -0.6 2,389,831 - -0.1 2,389,831 - -0.2 2,389,920 + -0.1 2,389,921 - -2.6 2,389,972 - -0.9 2,390,037 - +0.3 2,390,094 + -0.2 2,390,376 + RR42_RS31270 0.22 +1.6 2,390,376 + RR42_RS31270 0.22 +2.5 2,390,376 + RR42_RS31270 0.22 +1.4 2,390,376 + RR42_RS31270 0.22 +2.3 2,390,377 - RR42_RS31270 0.22 +1.0 2,390,377 - RR42_RS31270 0.22 -0.3 2,390,461 - RR42_RS31270 0.29 +2.4 2,390,461 - RR42_RS31270 0.29 +2.1 2,390,461 - RR42_RS31270 0.29 +0.9 2,390,461 - RR42_RS31270 0.29 +1.3
Or see this region's nucleotide sequence